Rabbit Anti-MCM4 Recombinant Antibody (clone R02-8F8) (CAT#: VS3-FY2030)
Clone R02-8F8 is a rabbit recombinant monoclonal antibody that is purified by affinity chromatography. It exhibits specific binding to human MCM4. It is suitable for use in a variety of assays, including WB, IP. This MCM4 antibody has been rigorously validated and is widely used in western blot analysis of MCM4 in Hela, A549, MCF-7, U251 lysates.
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WB
Figure 1 Anti-MCM4 Antibody (VS3-FY2030) in WB.
The MCM4 expression in Hela, A549, MCF-7, U251 lysates were detected using the anti-MCM4 by western blot assay.
Specifications
- Immunogen
- Synthetic peptide of human MCM4
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human MCM4
- Species Reactivity
- Human
- Clone
- R02-8F8
- Applications
- Western Blot, Immunoprecipitation
- MW
- The calculated weight of the target protein is approximately 97 kDa, while the observed molecular weight stands at 97 kDa.
- Related Disease
- Immunodeficiency 54 and Immunodeficiency 20
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunoprecipitation: 1/20
Target
- Alternative Names
- Minichromosome Maintenance Complex Component 4; CDC21 Homolog; P1-CDC21; CDC21; MCM4 Minichromosome Maintenance Deficient 4 (S. Cerevisiae); Homolog Of S. Pombe Cell Devision Cycle 21; Minichromosome Maintenance Deficient 4
- Gene ID
- 4173
- UniProt ID
- P33991
- Sequence Similarities
- Belongs to the MCM family.
- Cellular Localization
- Chromosome, Nucleus
- Post Translation Modifications
- Sumoylated; SUMO2 modified in response to stress caused by inhibition of proteasome activity (in vitro). (P33991-MCM4_HUMAN)
Ubiquitination at Lys123, Lys163, Lys179, Lys216, Lys220, Lys349, Lys381, Lys439, Lys444, Lys450, Lys536, Lys600, Lys628, Lys752, Lys762, Lys770, Lys814, Lys819, and Lys858 (NX_P33991 [NX_P33991-1])
Modification sites at PhosphoSitePlus (P33991)
Glycosylation from GlyGen (P33991) 2 sites, 1 O-linked glycan (2 sites)
- Protein Refseq
- NP_005905.2; NP_877423.1
- Function
- Acts as a component of the MCM2-7 complex (MCM complex) which is the replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells.
Recommended Products
Isotype Control
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2192 | Hi-Affi™ Recombinant Rabbit Anti-MCM4 Monoclonal Antibody (DS2192AB) | WB, IP, FC | IgG |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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