Rabbit Anti-ME2 Recombinant Antibody (clone R06-3F0) (CAT#: VS3-FY2031)

The R06-3F0 recombinant monoclonal antibody confidently identify human ME2 protein, a calculated weight of 65 kDa. It cross-reacts with mouse, rat. It is suitable for use in a variety of assays, including WB, ICC/IF.

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Custom Production

We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.

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  • Tested Data
  • Gene Expression
  • Datasheet
  • MSDS
  • COA
WB

Figure 1 Anti-ME2 Antibody (VS3-FY2031) in WB.

Figure 1 Anti-ME2 Antibody (VS3-FY2031) in WB.

The ME2 expression in mouse intestines, rat spleen lysates were detected using the anti-ME2 by western blot assay.

ICC

Figure 2 Anti-ME2 Antibody (VS3-FY2031) in ICC.

Figure 2 Anti-ME2 Antibody (VS3-FY2031) in ICC.

Immunocytochemistry staining of ME2 (green) in Jurkat using the anti-ME2 antibody. Nuclear DNA was labeled with DAPI (shown in blue).


Specifications

  • Immunogen
  • Synthetic peptide of human ME2
  • Host Species
  • Rabbit
  • Type
  • Rabbit IgG
  • Specificity
  • Human, Mouse and Rat ME2
  • Species Reactivity
  • Human, Mouse, Rat
  • Clone
  • R06-3F0
  • Applications
  • Western Blot, Immunofluorescence
  • MW
  • The calculated weight of the target protein is approximately 65 kDa, while the observed molecular weight stands at 65 kDa.
  • Related Disease
  • Opioid Dependence 1 and Epilepsy, Idiopathic Generalized

Product Property

  • Purification
  • Affinity Purified
  • Format
  • Liquid
  • Concentration
  • Please refer to the vial label for the specific concentration.
  • Buffer
  • 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% glycerol, 0.05% BSA
  • Preservative
  • 0.01% Sodium azide
  • Storage
  • Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Applications

  • Application Notes
  • Western Blot: 1/500-1/1000
    Immunofluorescence: 1/50-1/200

Target

  • Alternative Names
  • Malic Enzyme 2; Malate Dehydrogenase (Oxaloacetate-Decarboxylating); Malic Enzyme 2, NAD(+)-Dependent, Mitochondrial; NAD-Dependent Malic Enzyme, Mitochondrial; NAD-ME; Pyruvic-Malic Carboxylase; EC 1.1.1.38; EC 1.1.1; ODS1
  • Sequence Similarities
  • Belongs to the malic enzymes family.
  • Cellular Localization
  • Mitochondrion
  • Post Translation Modifications
  • Modification sites at PhosphoSitePlus (P23368)
    Modification sites at neXtProt (NX_P23368)
    Glycosylation from GlyGen (P23368) 2 sites, 1 O-linked glycan (2 sites)
  • Function
  • NAD-dependent mitochondrial malic enzyme that catalyzes the oxidative decarboxylation of malate to pyruvate.

Recommended Products

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

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CAT Product Name Application Type
MOR-2199 Hi-Affi™ Recombinant Rabbit Anti-ME2 Monoclonal Antibody (DS2199AB) WB, IHC, ICC IgG

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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