Rabbit Anti-METAP2 Recombinant Antibody (clone R07-6C1) (CAT#: VS3-FY2035)
The R07-6C1 recombinant monoclonal antibody was developed to identify human METAP2. It cross-reacts with rat. This methionine aminopeptidase 2 antibody has been rigorously validated and is widely used in western blot analysis of methionine aminopeptidase 2/p67 in C6 lysates and western blot analysis of methionine aminopeptidase 2/p67 in A549, HL-60, U251, U87-MG lysates.
Specific Inquiry
Custom Production
We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.
We require custom production
WB
Figure 1 Anti-METAP2 Antibody (VS3-FY2035) in WB.
The Methionine Aminopeptidase 2/p67 expression in C6 lysates were detected using the anti-Methionine Aminopeptidase 2 by western blot assay.
WB
Figure 2 Anti-METAP2 Antibody (VS3-FY2035) in WB.
The Methionine Aminopeptidase 2/p67 expression in A549, HL-60, U251, U87-MG lysates were detected using the anti-Methionine Aminopeptidase 2/p67 by western blot assay.
Specifications
- Immunogen
- Synthetic peptide of human methionine aminopeptidase 2/p67
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human and Rat METAP2
- Species Reactivity
- Human, Rat
- Clone
- R07-6C1
- Applications
- Western Blot
- MW
- The calculated weight of the target protein is approximately 53 kDa, while the observed molecular weight stands at 67 kDa.
- Related Disease
- Cancer
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Target
- Alternative Names
- Methionyl Aminopeptidase 2; Initiation Factor 2-Associated 67 KDa Glycoprotein; Peptidase M; P67eIF2; MNPEP; Testicular Tissue Protein Li 17; EIF-2-Associated P67 Homolog; Methionine Aminopeptidase 2
- Gene ID
- 10988
- UniProt ID
- P50579
- Sequence Similarities
- Belongs to the peptidase M24A family. Methionine aminopeptidase eukaryotic type 2 subfamily.
- Cellular Localization
- Cytoplasm
- Post Translation Modifications
- Contains approximately 12 O-linked N-acetylglucosamine (GlcNAc) residues.
O-glycosylation is required for EIF2S1 binding.
(P50579-MAP2_HUMAN)
Glycosylation at Ser60 and Ser63 (NX_P50579 [NX_P50579-1])
Ubiquitination at Lys289 and Lys427 (NX_P50579 [NX_P50579-1])
Modification sites at PhosphoSitePlus (P50579)
Glycosylation from GlyGen (P50579) 3 sites, 1 O-linked glycan (1 site)
- Protein Refseq
- NP_001304111.1; NP_001304112.1; NP_001317175.1; NP_006829.1
- Function
- Cotranslationally removes the N-terminal methionine from nascent proteins. The N-terminal methionine is often cleaved when the second residue in the primary sequence is small and uncharged (Met-Ala-, Cys, Gly, Pro, Ser, Thr, or Val). The catalytic activity of human METAP2 toward Met-Val peptides is consistently two orders of magnitude higher than that of METAP1, suggesting that it is responsible for processing proteins containing N-terminal Met-Val and Met-Thr sequences in vivo.
Recommended Products
Isotype Control
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "METAP2"
Select a product category from the dropdown menu below to view related products.
Please select product type
Rabbit Monoclonal Antibody Products
IgG Antibody Products
Antibody Magnetic Beads
Flow Cytometry (FC) related Reagents and Kits: Empowering Cell Function Research
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2218 | Hi-Affi™ Recombinant Rabbit Anti-METAP2 Monoclonal Antibody (DS2218AB) | WB, IHC, ICC, FC | IgG |
Customer Reviews and Q&As
There are currently no Customer reviews or questions for VS3-FY2035. Click the button above to contact us or submit your feedback about this product.
Popular products with customers
Application: IP, IF, FuncS, FC, Neut, ELISA, ICC
-2.png)
Application: ELISA, FC, IP, FuncS, IF, Neut, ICC
Application: IHC, ELISA, FC, WB, ADCC, FuncS
-CB2006C17L-4.jpg)
Application: WB, ELISA
-YJ5.png)
Application: WB, ELISA, FC, IHC, IP
Application: ELISA
Application: ELISA, IHC, FC, IP, IF, FuncS
For Research Use Only. Not For Clinical Use.
Send Inquiry
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
