Rabbit Anti-METAP2 Recombinant Antibody (clone R07-6C1) (CAT#: VS3-FY2035)
The R07-6C1 recombinant monoclonal antibody was developed to identify human METAP2. It cross-reacts with rat. This methionine aminopeptidase 2 antibody has been rigorously validated and is widely used in western blot analysis of methionine aminopeptidase 2/p67 in C6 lysates and western blot analysis of methionine aminopeptidase 2/p67 in A549, HL-60, U251, U87-MG lysates.
Specific Inquiry
WB
Figure 1 Anti-METAP2 Antibody (VS3-FY2035) in WB.
The Methionine Aminopeptidase 2/p67 expression in C6 lysates were detected using the anti-Methionine Aminopeptidase 2 by western blot assay.
WB
Figure 2 Anti-METAP2 Antibody (VS3-FY2035) in WB.
The Methionine Aminopeptidase 2/p67 expression in A549, HL-60, U251, U87-MG lysates were detected using the anti-Methionine Aminopeptidase 2/p67 by western blot assay.
Specifications
- Immunogen
- Synthetic peptide of human methionine aminopeptidase 2/p67
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human and Rat METAP2
- Species Reactivity
- Human, Rat
- Clone
- R07-6C1
- Applications
- Western Blot
- MW
- The calculated weight of the target protein is approximately 53 kDa, while the observed molecular weight stands at 67 kDa.
- Related Disease
- Cancer
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Target
- Alternative Names
- Methionyl Aminopeptidase 2; Initiation Factor 2-Associated 67 KDa Glycoprotein; Peptidase M; P67eIF2; MNPEP; Testicular Tissue Protein Li 17; EIF-2-Associated P67 Homolog; Methionine Aminopeptidase 2
- Gene ID
- 10988
- UniProt ID
- P50579
- Sequence Similarities
- Belongs to the peptidase M24A family. Methionine aminopeptidase eukaryotic type 2 subfamily.
- Cellular Localization
- Cytoplasm
- Post Translation Modifications
- Contains approximately 12 O-linked N-acetylglucosamine (GlcNAc) residues.
O-glycosylation is required for EIF2S1 binding.
(P50579-MAP2_HUMAN)
Glycosylation at Ser60 and Ser63 (NX_P50579 [NX_P50579-1])
Ubiquitination at Lys289 and Lys427 (NX_P50579 [NX_P50579-1])
Modification sites at PhosphoSitePlus (P50579)
Glycosylation from GlyGen (P50579) 3 sites, 1 O-linked glycan (1 site)
- Protein Refseq
- NP_001304111.1; NP_001304112.1; NP_001317175.1; NP_006829.1
- Function
- Cotranslationally removes the N-terminal methionine from nascent proteins. The N-terminal methionine is often cleaved when the second residue in the primary sequence is small and uncharged (Met-Ala-, Cys, Gly, Pro, Ser, Thr, or Val). The catalytic activity of human METAP2 toward Met-Val peptides is consistently two orders of magnitude higher than that of METAP1, suggesting that it is responsible for processing proteins containing N-terminal Met-Val and Met-Thr sequences in vivo.
Recommended Products
Isotype Control
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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See other products for "METAP2"
Rabbit Monoclonal Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2218 | Hi-Affi™ Recombinant Rabbit Anti-METAP2 Monoclonal Antibody (DS2218AB) | WB, IHC, ICC, FC | IgG |
Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-0991-CN | Recombinant Rabbit Anti-METAP2 Monoclonal Antibody (JG39-79) | WB, IF, IHC, FC | Rabbit IgG |
| MOB-278YJL | Rabbit Anti-METAP2 Recombinant Antibody (clone CBM263) | IHC-P | Rabbit IgG |
| ZG-0064U | Rabbit Anti-METAP2 Recombinant Antibody (clone 8F2) | ELISA, IHC | Rabbit IgG |
| VS3-WK498 | Rabbit Anti-METAP2 Recombinant Antibody (clone JG39-79) | WB, ELISA | Rabbit IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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