Rabbit Anti-METAP2 Recombinant Antibody (clone R07-6C1)
CAT#: VS3-FY2035
The R07-6C1 recombinant monoclonal antibody was developed to identify human METAP2. It cross-reacts with rat. This methionine aminopeptidase 2 antibody has been rigorously validated and is widely used in western blot analysis of methionine aminopeptidase 2/p67 in C6 lysates and western blot analysis of methionine aminopeptidase 2/p67 in A549, HL-60, U251, U87-MG lysates.
Gene Expression
Tested Data
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane & cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/19095/if_selected.jpg
Normal Tissue
Figure 2 Testis
Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Pachytene spermatocytes Staining: High Intensity: Strong Quantity: 75%-25% Preleptotene spermatocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Round or early spermatids Staining: High Intensity: Strong Quantity:>75% Spermatogonia cells Staining: Medium Intensity: Moderate
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/19095/50082_A_4_6.jpg
Normal Tissue
Figure 3 Seminal vesicle
Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/19095/50082_A_7_6.jpg
Normal Tissue
Figure 4 Lymph node
Germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/19095/50082_A_8_8.jpg
Normal Tissue
Figure 5 Colon
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/19095/50082_A_9_3.jpg
Normal Tissue
Figure 6 Liver
Cholangiocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/19095/50082_A_8_4.jpg
Normal Tissue
Figure 7 Kidney
Cells in glomeruli Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Cells in tubules Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/19095/50082_A_7_5.jpg
RNA Expression
Figure 8 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000111142-METAP2
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WB
Figure 1 Anti-METAP2 Antibody (VS3-FY2035) in WB.
The Methionine Aminopeptidase 2/p67 expression in C6 lysates were detected using the anti-Methionine Aminopeptidase 2 by western blot assay.
WB
Figure 2 Anti-METAP2 Antibody (VS3-FY2035) in WB.
The Methionine Aminopeptidase 2/p67 expression in A549, HL-60, U251, U87-MG lysates were detected using the anti-Methionine Aminopeptidase 2/p67 by western blot assay.
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Specifications
- Immunogen
- Synthetic peptide of human methionine aminopeptidase 2/p67
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human and Rat METAP2
- Species Reactivity
- Human, Rat
- Clone
- R07-6C1
- Applications
- Western Blot
- MW
- The calculated weight of the target protein is approximately 53 kDa, while the observed molecular weight stands at 67 kDa.
- Related Disease
- Cancer
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Target
- Alternative Names
- Methionyl Aminopeptidase 2; Initiation Factor 2-Associated 67 KDa Glycoprotein; Peptidase M; P67eIF2; MNPEP; Testicular Tissue Protein Li 17; EIF-2-Associated P67 Homolog; Methionine Aminopeptidase 2
- Gene ID
- 10988
- UniProt ID
- P50579
- Sequence Similarities
- Belongs to the peptidase M24A family. Methionine aminopeptidase eukaryotic type 2 subfamily.
- Cellular Localization
- Cytoplasm
- Post Translation Modifications
- Contains approximately 12 O-linked N-acetylglucosamine (GlcNAc) residues.
O-glycosylation is required for EIF2S1 binding.
(P50579-MAP2_HUMAN)
Glycosylation at Ser60 and Ser63 (NX_P50579 [NX_P50579-1])
Ubiquitination at Lys289 and Lys427 (NX_P50579 [NX_P50579-1])
Modification sites at PhosphoSitePlus (P50579)
Glycosylation from GlyGen (P50579) 3 sites, 1 O-linked glycan (1 site)
- Protein Refseq
- NP_001304111.1; NP_001304112.1; NP_001317175.1; NP_006829.1
- Function
- Cotranslationally removes the N-terminal methionine from nascent proteins. The N-terminal methionine is often cleaved when the second residue in the primary sequence is small and uncharged (Met-Ala-, Cys, Gly, Pro, Ser, Thr, or Val). The catalytic activity of human METAP2 toward Met-Val peptides is consistently two orders of magnitude higher than that of METAP1, suggesting that it is responsible for processing proteins containing N-terminal Met-Val and Met-Thr sequences in vivo.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "METAP2"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2218 | Hi-Affi™ Recombinant Rabbit Anti-METAP2 Monoclonal Antibody (DS2218AB) | WB, IHC, ICC, FC | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-0991-CN | Recombinant Rabbit Anti-METAP2 Monoclonal Antibody (JG39-79) | WB, IF, IHC, FC | Rabbit IgG |
| MOB-278YJL | Rabbit Anti-METAP2 Recombinant Antibody (clone CBM263) | IHC-P | Rabbit IgG |
| ZG-0064U | Rabbit Anti-METAP2 Recombinant Antibody (clone 8F2) | ELISA, IHC | Rabbit IgG |
| VS3-WK498 | Rabbit Anti-METAP2 Recombinant Antibody (clone JG39-79) | WB, ELISA | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC1511 | AbPlus™ Anti-Metap2 Magnetic Beads (VS-0724-YC1511) | IP, Protein Purification | |
| VS-0724-YC1514 | AbPlus™ Anti-METAP2 Magnetic Beads (VS-0724-YC1514) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS13-YC724 | CytoStream™ Rabbit Anti-METAP2 Recombinant Antibody (VS13-YC724) | WB, ICC, IF, IHC-P, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY4358 | Anti-Mouse METAP2 Immunohistochemistry Kit | IHC |
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