CytoStream™ Rabbit Anti-METAP2 Recombinant Antibody (VS13-YC724)
CAT#: VS13-YC724
The CytoStream™ Rabbit Anti-METAP2 Recombinant Antibody is specific for human, mouse and rat METAP2. METAP2 encodes a methionyl aminopeptidase inhibiting phosphorylations, active site-bound by anti-cancer drugs like fumagillin, with various splicing variants including several pseudogenes. The anti-METAP2 antibody was developed and selected by our CytoStream™ platform and the mAb is recommended for use in flow cytometry staining (1/50-1/100).
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane & cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/19095/if_selected.jpg
Normal Tissue
Figure 2 Testis
Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Pachytene spermatocytes Staining: High Intensity: Strong Quantity: 75%-25% Preleptotene spermatocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Round or early spermatids Staining: High Intensity: Strong Quantity:>75% Spermatogonia cells Staining: Medium Intensity: Moderate
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/19095/50082_A_4_6.jpg
Normal Tissue
Figure 3 Seminal vesicle
Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/19095/50082_A_7_6.jpg
Normal Tissue
Figure 4 Lymph node
Germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/19095/50082_A_8_8.jpg
Normal Tissue
Figure 5 Colon
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/19095/50082_A_9_3.jpg
Normal Tissue
Figure 6 Liver
Cholangiocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/19095/50082_A_8_4.jpg
Normal Tissue
Figure 7 Kidney
Cells in glomeruli Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Cells in tubules Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/19095/50082_A_7_5.jpg
RNA Expression
Figure 8 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000111142-METAP2
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Specifications
- Immunogen
- Recombinant human Methionine Aminopeptidase 2, aa 340-478.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat METAP2
- Species Reactivity
- Human, Mouse, Rat
- Applications
- WB, ICC, IF, IHC-P, FC
- Conjugate
- NONE
- Related Disease
- Cancer
Product Property
- Clonality
- Monoclonal
- Purification
- Protein A purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- TBS (pH 7.4) containing 40% Glycerol and 0.05% BSA.
- Preservative
- 0.05% Sodium azide
- Storage
- Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Shipping with ice pack.
Applications
- Application Notes
- Western blot (WB): 1/500-1/2000
Immunocytochemistry (ICC): 1/50-1/200
Immunofluorescence (IF): 1/50-1/200
Immunohistochemistry-paraffin (IHC-P): 1/50-1/200
Flow cytometry (FC): 1/50-1/100
Target
- Alternative Names
- Methionyl Aminopeptidase 2; Initiation Factor 2-Associated 67 KDa Glycoprotein; Peptidase M; P67eIF2; MNPEP; Testicular Tissue Protein Li 17; EIF-2-Associated P67 Homolog; Methionine Aminopeptidase 2;
- Gene ID
- 10988
- UniProt ID
- P50579
- Long Name
- Methionyl Aminopeptidase 2
- Sequence Similarities
- Belongs to the peptidase M24A family. Methionine aminopeptidase eukaryotic type 2 subfamily.
- Cellular Localization
- Cytoplasm
- Protein Refseq
- NP_001304111.1; NP_001304112.1; NP_001317175.1; NP_006829.1
- Function
- Cotranslationally removes the N-terminal methionine from nascent proteins. The N-terminal methionine is often cleaved when the second residue in the primary sequence is small and uncharged (Met-Ala-, Cys, Gly, Pro, Ser, Thr, or Val). The catalytic activity of human METAP2 toward Met-Val peptides is consistently two orders of magnitude higher than that of METAP1, suggesting that it is responsible for processing proteins containing N-terminal Met-Val and Met-Thr sequences in vivo.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "METAP2"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2218 | Hi-Affi™ Recombinant Rabbit Anti-METAP2 Monoclonal Antibody (DS2218AB) | WB, IHC, ICC, FC | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-0991-CN | Recombinant Rabbit Anti-METAP2 Monoclonal Antibody (JG39-79) | WB, IF, IHC, FC | Rabbit IgG |
| MOB-278YJL | Rabbit Anti-METAP2 Recombinant Antibody (clone CBM263) | IHC-P | Rabbit IgG |
| ZG-0064U | Rabbit Anti-METAP2 Recombinant Antibody (clone 8F2) | ELISA, IHC | Rabbit IgG |
| VS3-WK498 | Rabbit Anti-METAP2 Recombinant Antibody (clone JG39-79) | WB, ELISA | Rabbit IgG |
| VS3-FY2035 | Rabbit Anti-METAP2 Recombinant Antibody (clone R07-6C1) | WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC1511 | AbPlus™ Anti-Metap2 Magnetic Beads (VS-0724-YC1511) | IP, Protein Purification | |
| VS-0724-YC1514 | AbPlus™ Anti-METAP2 Magnetic Beads (VS-0724-YC1514) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY4358 | Anti-Mouse METAP2 Immunohistochemistry Kit | IHC |
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