Mouse Anti-MICB Recombinant Antibody (clone 1B2F11)
CAT#: VS7-0425-WR384
This product is a mouse IgG1 antibody that binds to human MICB. The antibody 1B2F11 recognizes specific amino acid domain of human MICB (AA: extra 23-309). It can be used for FC assay.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line BJ
Immunofluorescent staining of human cell line BJ shows localization to nucleoplasm, plasma membrane & the Golgi apparatus.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/64618/1446_E9_5_selected.jpg
RNA Expression
Figure 2 RNA cell line category: Cell line enhanced (U-266/70, U-266/84)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000204516-MICB
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Specifications
- Immunogen
- Recombinant human MICB (AA: extra 23-309) fragment expressed in E. Coli.
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Human MICB
- Species Reactivity
- Human
- Clone
- 1B2F11
- Molecule Class
- Non-Classical Mhc Class I-Related Molecule
- Applications
- FC
- Conjugate
- Unconjugated
- Related Disease
- Rheumatoid arthritis (RA)
Product Property
- Clonality
- Monoclonal
- Purity
- >95% as determined by SDS-PAGE and SEC-HPLC analysis
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- 0.05% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- Western Blot: 1:500 - 1:2000
Flow Cytometry: 1:200 - 1:400
Enzyme-linked Immunosorbent Assay: 1:10000
Target
- Alternative Names
- MICB; MIC-B; MHC Class I-Like Molecule PERB11.2-IMX; MHC Class I Chain-Related Protein B; PERB11.2; Stress Inducible Class I Homolog; MHC Class I Mic-B Antigen; MHC Class I Polypeptide-Related Sequence B
- Gene ID
- 4277
- UniProt ID
- Q29980
- Long Name
- MHC Class I Polypeptide-Related Sequence B
- Cellular Localization
- Cell membrane; Membrane
- Post Translation Modifications
- Disulfide bond; Glycoprotein
- Protein Refseq
- NP_001276089.1
- Function
- Widely expressed membrane-bound protein which acts as a ligand to stimulate an activating receptor KLRK1/NKG2D, expressed on the surface of essentially all human natural killer (NK), gammadelta T and CD8+ alphabeta T-cells (PubMed:11491531, PubMed:11777960).; Up-regulated in stressed conditions, such as viral and bacterial infections or DNA damage response, serves as signal of cellular stress, and engagement of KLRK1/NKG2D by MICA triggers NK-cells resulting in a range of immune effector functions, such as cytotoxicity and cytokine production
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Isotype Control
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- Product Name
Secondary Antibody
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Reconstitution Buffer
- CAT
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Recommended Dilution Buffer
- CAT
- Product Name
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-3208z | Mouse Anti-MICB Recombinant Antibody (clone 25D2) | WB, IP, IF, ELISA | Mouse IgG1, κ |
| MOB-1235CT | Recombinant Mouse anti-Human MICB Monoclonal antibody (NN0584-4D48) | FC, WB | |
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| CAT | Product Name | Application | Type |
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| NEUT-1740CQ | Recombinant Mouse Anti-MICB Antibody (CBL145) | FC, WB, IHC, Neut | IgG2 |
| CAT | Product Name | Application | Type |
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| MOR-4328 | Hi-Affi™ Recombinant Rabbit Anti-MICB Monoclonal Antibody (SI417DS) | WB | IgG |
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-N0178-YC-S(P) | Mouse Anti-MICB Recombinant Antibody; scFv Fragment (HPAB-N0178-YC-S(P)) | ELISA, FC | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-N0178-YC-F(E) | Mouse Anti-MICB Recombinant Antibody; Fab Fragment (HPAB-N0178-YC-F(E)) | ELISA, FC | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-YC458 | Recombinant Anti-MICB Vesicular Antibody, EV Displayed (VS-0425-YC458) | ELISA, FC, Cell-uptake |
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