Rabbit Anti-MLKL Recombinant Antibody (clone R05-3H2) (CAT#: VS3-XY3429)
This antibody is a recombinant rabbit antibody against MLKL (Mixed lineage kinase domain like pseudokinase), which plays a key role in TNF-induced necroptosis, a programmed cell death process. It specifically reacts with human and mouse MLKL. It has been reported for use in Western Blot and Immunohistochemistry-Paraffin. The recommended dilutions of Western Blot and Immunohistochemistry-Paraffin is 1/500-1/1000 and 1/50-1/100, respectively.
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Subcellular Location
RNA Expression
Figure 1 IF staining of human cell line HUVEC TERT2
(Immunofluorescent staining of human cell line HUVEC TERT2 shows localization to plasma membrane & cell junctions.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/78638/1751_F6_8_cr57fd37317cd94_selected.jpg
(Immunofluorescent staining of human cell line HUVEC TERT2 shows localization to plasma membrane & cell junctions.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/78638/1751_F6_8_cr57fd37317cd94_selected.jpg
Figure 2 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000168404-MLKL
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000168404-MLKL
Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human and Mouse MLKL
- Species Reactivity
- Human, Mouse
- Clone
- R05-3H2
- Applications
- WB, IHC-P
- Related Disease
- Maturity-Onset Diabetes of The Young and Inflammatory Bowel Disease
Product Property
- Clonality
- Monoclonal
- Aggregation
- < 5%
- Format
- Liquid
- Buffer
- PBS (pH 7.4), 150 mM NaCl and 50% glycerol
- Preservative
- 0.02% sodium azide
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- WB: 1/500-1/1000
IHC: 1/50-1/100
Target
- Alternative Names
- hMLKL
- Gene ID
- 197259
- UniProt ID
- Q8NB16
- Long Name
- Mixed lineage kinase domain like pseudokinase
- Cellular Localization
- Cytoplasm, Cell membrane, Nucleus
- Post Translation Modifications
- Phosphorylation by RIPK3 induces a conformational switch that is required for necroptosis (PubMed:22265413).
It also induces homotrimerization and localization to the plasma membrane (PubMed:22265413).
(Q8NB16-MLKL_HUMAN)
Modification sites at PhosphoSitePlus (Q8NB16)
Modification sites at neXtProt (NX_Q8NB16)
Glycosylation from GlyGen (Q8NB16) 4 sites, 1 O-linked glycan (4 sites)
- Protein Refseq
- XP_011521238.1; XP_005255891.1
- Function
- Pseudokinase that plays a key role in TNF-induced necroptosis, a programmed cell death process (PubMed:22265413, 22265414, 22421439, 24316671).
Does not have protein kinase activity (PubMed:22265413, 22265414, 22421439, 24316671).
Activated following phosphorylation by RIPK3, leading to homotrimerization, localization to the plasma membrane and execution of programmed necrosis characterized by calcium influx and plasma membrane damage.
Recommended Products
Isotype Control
Recommended Dilution Buffer
Secondary Antibody
Related Resources
TNF Signaling Pathway
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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IgG Antibody Products
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IHC Kit and Antibody Products: Precision Tools for Immunohistochemistry
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-1006-CN | Recombinant Rabbit Anti-MLKL Monoclonal Antibody (CBACN-374) | WB, IHC | Rabbit IgG |
| MRO-2347-CN | Recombinant Rabbit Anti-MLKL (phosphorylated Ser345) Monoclonal Antibody (JM92-37) | WB, IHC, IP | Rabbit IgG |
| VS3-QX774 | Mouse Anti-MLKL Recombinant Antibody (clone 10F6-8G10-2C6) | IHC | Mouse IgG1 |
| VS3-QX775 | Mouse Anti-MLKL Recombinant Antibody (clone 5E3-6B4-7G9) | IHC | Mouse IgG1 |
| VS13-YC732 | Rabbit Anti-MLKL Recombinant Antibody (VS13-YC732) | WB, IHC-P | Rabbit IgG |
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