Human Anti-Modified citrulline Recombinant Antibody (clone C4)
CAT#: ZG-454C
This product is a human antibody that recognizes modified citrulline. It can be used for ELISA and WB.
Tested Data
ELISA
Figure 1 Human Anti-Modified citrulline Antibody (ZG-454C) in ELISA
Antibody diluted to 0.4 μg/ml was tested on various modified citrulline-containing peptides and their non-modified forms.
WB
Figure 2 Human Anti-Modified citrulline Antibody (ZG-454C) in WB
A) After blotting, the proteins on the membrane (fibrinogen, soybean trypsin inhibitor (STI), histones and their de-iminated forms) were chemically modified with 2,3-butanedione monoxime and antipyrine. Subsequently, the membrane was probed with AMC antibody. The right panel shows the corresponding SDS-PAGE gel stained with coomassie. B) AMC antibody was tested on 10 μg HEp-2 cell lysate and 10 μg of its de-iminated form after chemical modification of the proteins on the membrane with 2,3-butanedione monoxime and antipyrine (left panel). As control (de-iminated) STI was loaded. The right panel shows the corresponding SDS-PAGE gel stained with coomassie.
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Specifications
- Immunogen
- Citrulline-containing peptide modified with 2,3-butanedione monoxime and antipyrine.
- Host Species
- Human
- Type
- Human IgG1, λ
- Antibody Isotype
- IgG1, λ
- Specificity
- Recognizes citrulline-containing proteins modifiedwith 2,3-butanedione monoxime and antipyrine regardless of neighbouring amino acid sequences.
- Clone
- C4
- Applications
- ELISA, WB
Product Property
- Purification
- Protein A affinity purified
- Purity
- >95% as deterimined by SDS-PAGE
- Format
- Liquid
- Buffer
- PBS
- Storage
- Store at 4°C for short term. For long term storage, store at-20°C, avoiding freeze/thaw cycles.
Applications
- Application Notes
- ELISA 0.08 - 0.4 µg/ml. WB 1 µg/ml
Target
- Alternative Names
- Citrulline, modified
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Detects Citrullinated Proteins
Clone C4 is unique because it recognizes modified citrulline residues regardless of the neighboring amino acid sequence. We used it in Western Blot at 1 µg/ml and it successfully detected proteins modified by PADs in our samples.
High Specificity In ELISA
We employed this human recombinant antibody in ELISA to screen for citrulline-containing peptides modified with 2,3-butanedione monoxime and antipyrine. It showed excellent specificity and low background. It is an essential reagent for our study of protein deimination.
Q&As
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Does this antibody recognize a specific amino acid sequence?
A: No, clone C4 recognizes citrulline-containing proteins modified with 2,3-butanedione monoxime and antipyrine regardless of the neighboring amino acid sequences.
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How are citrulline residues generated in proteins?
A: Citrulline residues are generated by peptidylarginine deiminases (PADs), which convert arginine into citrulline through a process called citrullination or deimination.
View the frequently asked questions answered by Creative Biolabs Support.
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Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
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COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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See other products for "Modified citrulline"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| ZG-453C | Mouse Anti-Modified citrulline Recombinant Antibody (clone 4CS) | ELISA, WB | Mouse IgG2a, κ |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| ZG-455C | Human Anti-Modified citrulline Recombinant Antibody (clone C4S) | ELISA, WB | Human IgG1, κ |
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