Human Anti-Modified citrulline Recombinant Antibody (clone C4S) (CAT#: ZG-455C)
This product is a human antibody that recognizes modified citrulline. It can be used for ELISA and WB.
Specific Inquiry
ELISA
Figure 1 Human Anti-Modified citrulline Antibody (ZG-455C) in ELISA
Antibody diluted to 0.4 μg/ml was tested on various modified citrulline-containing peptides and their non-modified forms.
WB
Figure 2 Human Anti-Modified citrulline Antibody (ZG-455C) in WB
A) After blotting, the proteins on the membrane (fibrinogen, soybean trypsin inhibitor (STI), histones and their de-iminated forms) were chemically modified with 2,3-butanedione monoxime and antipyrine. Subsequently, the membrane was probed with AMC antibody. The right panel shows the corresponding SDS-PAGE gel stained with coomassie. B) AMC antibody was tested on 10 μg HEp-2 cell lysate and 10 μg of its de-iminated form after chemical modification of the proteins on the membrane with 2,3-butanedione monoxime and antipyrine (left panel). As control (de-iminated) STI was loaded. The right panel shows the corresponding SDS-PAGE gel stained with coomassie.
Specifications
- Immunogen
- Citrulline-containing peptide modified with 2,3-butanedione monoxime and antipyrine.
- Host Species
- Human
- Type
- Human IgG1, κ
- Specificity
- Recognizes citrulline-containing proteins modifiedwith 2,3-butanedione monoxime and antipyrine regardless of neighbouring amino acid sequences.
- Clone
- C4S
- Applications
- ELISA, WB
Product Property
- Purification
- Protein A affinity purified
- Purity
- >95% as deterimined by SDS-PAGE
- Format
- Lyophilized
- Storage
- Store at 4°C for short term. For long term storage, store at-20°C, avoiding freeze/thaw cycles.
Applications
- Application Notes
- ELISA 0.08 - 0.4 µg/ml. WB 1 µg/ml
Target
- Alternative Names
- Citrulline, modified
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| ZG-453C | Mouse Anti-Modified citrulline Recombinant Antibody (clone 4CS) | ELISA, WB | Mouse IgG2a, κ |
| ZG-454C | Human Anti-Modified citrulline Recombinant Antibody (clone C4) | ELISA, WB | Human IgG1, λ |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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