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    Mouse Anti-MSH6 Recombinant Antibody (clone 5B11) (CAT#: VS7-0425-WR422)

    This product is a mouse IgG1 antibody that binds to human MSH6. The antibody 5B11 can be used for WB assay.

    Specific Inquiry
    • Size:

    • Conjugation:

    • Endotoxin:

    • Purity:

    • Formats:

    • Isotype Switching:

    • Fc Engineering:

    • Modalities:

    Custom Production

    We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.

    Sequences:
    Isotype :
    Purity :
    Endotoxin :
    Quantity :
    Conjugation :
    Fc Engineering :
    Modalities :
    Other Requirements:
    We require custom production
    • Gene Expression
    • Datasheet
    • MSDS
    • COA
    Subcellular Location and Protein Expression
    Figure 1 IF staining of human cell line U-2 OS
    Figure 1 IF staining of human cell line U-2 OS
    (Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & vesicles.)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/28376/if_selected.jpg
    Figure 2 IHC staining of human lymph node
    Figure 2 IHC staining of human lymph node
    (Immunohistochemical staining of human lymph node shows strong nuclear positivity in reaction center cells.)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/9091/ihc_selected.jpg
    Figure 3 IHC staining of human colon
    Figure 3 IHC staining of human colon
    (Immunohistochemical staining of human colon shows strong nuclear positivity in glandular cells.)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/70870/ihc_selected.jpg
    Figure 4 IF staining of human cell line A-431
    Figure 4 IF staining of human cell line A-431
    (Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm, golgi apparatus & cytosol.)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/28376/290_E8_2_red_green.jpg
    Figure 5 IF staining of human cell line U-251 MG
    Figure 5 IF staining of human cell line U-251 MG
    (Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & vesicles.)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/28376/249_E8_1_red_green.jpg
    Normal Tissue
    Figure 6 Cerebral cortex
    Figure 6 Cerebral cortex
    (Glial cells
    Staining:High
    Intensity: Strong
    Quantity:>75%
    Location: Nuclear
    Neuronal cells
    Staining:High
    Intensity: Strong
    Quantity: 75%-25%
    Location: Nuclear)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/70870/151813_B_8_5.jpg
    Figure 7 Colon
    Figure 7 Colon
    (Endothelial cells
    Staining:Medium
    Intensity: Moderate
    Quantity:>75%
    Location: Nuclear
    Glandular cells
    Staining:High
    Intensity: Strong
    Quantity: 75%-25%
    Location: Nuclear
    Peripheral nerve/ganglion
    Staining:Medium
    Intensity: Moderate
    Quantity:>75%
    Location: Nuclear)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/70870/151813_A_7_3.jpg
    Figure 8 Liver
    Figure 8 Liver
    (Cholangiocytes
    Staining:Medium
    Intensity: Moderate
    Quantity: 75%-25%
    Location: Nuclear
    Hepatocytes
    Staining:Medium
    Intensity: Moderate
    Quantity: 75%-25%
    Location: Nuclear)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/70870/151813_A_9_4.jpg
    Figure 9 Kidney
    Figure 9 Kidney
    (Cells in glomeruli
    Staining:High
    Intensity: Strong
    Quantity: 75%-25%
    Location: Nuclear
    Cells in tubules
    Staining:High
    Intensity: Strong
    Quantity:>75%
    Location: Nuclear)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/70870/151813_A_8_5.jpg
    Figure 10 Testis
    Figure 10 Testis
    (Elongated or late spermatids
    Staining:High
    Intensity: Strong
    Quantity:>75%
    Leydig cells
    Staining:High
    Intensity: Strong
    Quantity:>75%
    Pachytene spermatocytes
    Staining:High
    Intensity: Strong
    Quantity:>75%
    Peritubular cells
    Staining:High
    Intensity: Strong
    Quantity:>75%
    Preleptotene spermatocytes
    Staining:High
    Intensity: Strong
    Quantity:>75%
    Round or early spermatids
    Staining:High
    Intensity: Strong
    Quantity:>75%
    Sertoli cells
    Staining:High
    Intensity: Strong
    Quantity:>75%
    Spermatogonia cells
    Staining:High
    Intensity: Strong
    Quantity:>75%)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/70870/151813_A_6_6.jpg
    Figure 11 Ovary
    Figure 11 Ovary
    (Ovarian stroma cells
    Staining:High
    Intensity: Strong
    Quantity:>75%
    Location: Nuclear)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/70870/151813_A_4_7.jpg
    Figure 12 Lymph node
    Figure 12 Lymph node
    (Germinal center cells
    Staining:High
    Intensity: Strong
    Quantity:>75%
    Location: Nuclear
    Non-germinal center cells
    Staining:Medium
    Intensity: Moderate
    Quantity: 75%-25%
    Location: Nuclear)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/70870/151813_A_9_8.jpg
    RNA Expression
    Figure 13 RNA cell line category: Low cell line specificity
    Figure 13 RNA cell line category: Low cell line specificity
    (Cell lines ordered by descending RNA expression order.)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000116062-MSH6

    Specifications

    • Immunogen
    • Recombinant human MSH6 fragment expressed in E. Coli.
    • Host Species
    • Mouse
    • Type
    • Mouse IgG1
    • Specificity
    • Human MSH6
    • Species Reactivity
    • Human
    • Clone
    • 5B11
    • Molecule Class
    • Dna Mismatch Repair Protein
    • Applications
    • WB
    • Conjugate
    • Unconjugated
    • Related Disease
    • Lynch syndrome 5 (LYNCH5); Endometrial cancer (ENDMC); Mismatch repair cancer syndrome 3 (MMRCS3); Colorectal cancer (CRC)

    Product Property

    • Clonality
    • Monoclonal
    • Purity
    • >95% as determined by SDS-PAGE and SEC-HPLC analysis, Ascitic fluid
    • Format
    • Liquid
    • Concentration
    • Please refer to the vial label for the specific concentration.
    • Preservative
    • 0.03% sodium azide
    • Storage
    • Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
    • Shipping
    • Ice packs

    Applications

    • Application Notes
    • Western Blot: 1:500 - 1:2000
      Enzyme-linked Immunosorbent Assay: 1:10000

    Target

    • Alternative Names
    • MSH6; mutS homolog 6; GTBP; HSAP; p160; GTMBP; HNPCC5; DNA mismatch repair protein Msh6; G/T mismatch-binding protein; mutS-alpha 160 kDa subunit; sperm-associated protein
    • Long Name
    • MutS Homolog 6
    • Cellular Localization
    • Chromosome; Nucleus
    • Post Translation Modifications
    • Acetylation; Phosphoprotein
    • Function
    • DNA-binding

    Recommended Products

    Isotype Control

    Secondary Antibody

    Product Notes

    This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

    • Increased sensitivity
    • Confirmed specificity
    • High repeatability
    • Excellent batch-to-batch consistency
    • Sustainable supply
    • Animal-free production

    See more details about Hi-Affi™ recombinant antibody benefits.

    Downloads

    Download resources about recombinant antibody development and antibody engineering to boost your research.

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    CAT Product Name Application Type
    DrMAB-407 Recombinant Human Anti-Human MSH6 Antibody Fab Fragment, clone D205 IP, IF, ELISA, IP-MS Fab

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