Mouse Anti-Myc tag Recombinant Antibody (ZG-0282U) (CAT#: ZG-0282U)
Mouse anti-Myc tag antibody displayed high specificity against the Myc tag.
Specific Inquiry
IF
Figure 2 Mouse Anti-Myc tag Antibody (ZG-0282U) in IF
Immunofluorescence staining of transfected HEK293 cells with ZG-0282U at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated Goat Anti-Mouse IgG(H+L).
IP
Figure 3 Mouse Anti-Myc tag Antibody (ZG-0282U) in IP
Immunoprecipitating MYC-tag with transfected HEK293 Lane 1: Mouse control IgG (1µg) in transfected HEK293 whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2: ZG-0282U (6µg) + transfected HEK293 whole cell lysate (1mg)
Lane 3: Transfected HEK293 whole cell lysate (10µg)
WB
Figure 1 Mouse Anti-Myc tag Antibody (ZG-0282U) in WB
Mouse anti Myc-tagged fusion protein Monoclonal antibody at 1.6 μg/ml
Lane 1: Recombinant Myc-tagged fusion protein at 50 ng
Lane 2: Recombinant Myc-tagged fusion protein at 25 ng
Lane 3: Recombinant Myc-tagged fusion protein at 12.25 ng
Lane 4: Recombinant Myc-tagged fusion protein at 6.25 ng
Lane 5: Recombinant Myc-tagged fusion protein at 3.125 ng
Lane 6: Recombinant Myc-tagged fusion protein at 1.5625 ng
Secondary
Goat polyclonal to Mouse IgG at 1/50000 dilution
Predicted band size: 50 kd
Observed band size: 50 kd
Specifications
- Immunogen
- EQKLISEEDL (Myc) synthetic peptide conjugate to KLH
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Myc tag
- Species Reactivity
- All
- Applications
- ELISA, WB, IF, IP
- Conjugate
- Unconjugated
Product Property
- Purification
- Protein G purified
- Purity
- >95% as determined by SDS-PAGE
- Buffer
- PBS, pH 7.4, 0.03% Proclin 300, 50% glycerol.
- Preservative
- 0.03% Proclin 300
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Enzyme-linked Immunosorbent Assay, Western Blot, Immunofluorescence, Immunoprecipitation. Specific conditions should be optimized by the end user.
WB:1:500-1:5000, IF:1:100-1:300, IP:1:1000-1:1500
Target
- Alternative Names
- bHLHe39; c Myc; MRTL; MYC; Myc proto oncogene protein; MYC tag; Proto oncogene c Myc; Transcription factor p64
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-1048-CN | Recombinant Mouse Anti-Myc Tag Monoclonal Antibody (A3-B4) | WB, ELISA, IP | Mouse IgG2b |
| VS3-WK1263 | Mouse Anti-Myc Tag Recombinant Antibody (VS3-WK1263) | WB, ICC, IF, IP | Mouse IgG1 |
| VS3-WK1400 | Mouse Anti-Myc Tag Recombinant Antibody (clone 3E8) | WB | Mouse IgG1 |
| VS3-WK1557 | Mouse Anti-Myc Tag Recombinant Antibody (clone 5D6) | WB, IP, IF | Mouse IgG1 |
| VS3-WK1566 | Mouse Anti-Myc Tag Recombinant Antibody (clone 170) | WB, IP, IF | Mouse IgG1 |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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