Rabbit Anti-MYL12B Recombinant Antibody (clone R14-2G-3)
CAT#: VS3-FY2170
Clone R14-2G-3 is a rabbit recombinant monoclonal antibody that demonstrated a greater affinity to human MYL12B protein, a calculated weight of 20 kDa. It cross-reacts with mouse, rat.
The following recommendations serve as a starting point for assay optimization. The actual working concentration may vary and should be determined by the user. The R14-2G-3 is recommended for detection of target protein by western blotting (dilution range 1:500-1:1000), immunohistochemistry (dilution range 1:100-1:200), immunofluorescence (dilution range 1:50-1:200).
Gene Expression
Normal Tissue
Figure 1 Cerebral cortex
Neuropil Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/59197/133434_B_7_5.jpg
Normal Tissue
Figure 2 Smooth muscle
Smooth muscle cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/39262/109225_B_5_7.jpg
RNA Expression
Figure 3 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000118680-MYL12B
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Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat MYL12B
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R14-2G-3
- Applications
- Western Blot, Immunohistochemistry-Paraffin, Immunofluorescence
- Conjugate
- Unconjugated
- MW
- The calculated weight of the target protein is approximately 20 kDa, while the observed molecular weight stands at 18kDa.
- Related Disease
- Megalencephalic Leukoencephalopathy With Subcortical Cysts
Product Property
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS (pH 7.4), 150 mM NaCl, 50% glycerol
- Preservative
- 0.02% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry: 1/100-1/200
Immunofluorescence: 1/50-1/200
Target
- Alternative Names
- Myosin Light Chain 12B; Myosin Regulatory Light Chain 2-B, Smooth Muscle Isoform; Myosin Regulatory Light Chain 20 KDa; Myosin, Light Chain 12B, Regulatory; Myosin Regulatory Light Chain MRLC2; Myosin Regulatory Light Chain 2; SHUJUN-1; MLC-2A
- Gene ID
- 103910
- UniProt ID
- O14950
- Post Translation Modifications
- Phosphorylation increases the actin-activated myosin ATPase activity and thereby regulates the contractile activity.
It is required to generate the driving force in the migration of the cells but not necessary for localization of myosin-2 at the leading edge.
Phosphorylation is reduced following epigallocatechin-3-O-gallate treatment.
(O14950-ML12B_HUMAN)
Modification sites at PhosphoSitePlus (O14950)
Modification sites at neXtProt (NX_O14950)
Glycosylation from GlyGen (O14950) 1 site, 1 O-linked glycan (1 site)
- Protein Refseq
- NP_001138416.1; NP_001138417.1; NP_291024.1
- Function
- Myosin regulatory subunit that plays an important role in regulation of both smooth muscle and nonmuscle cell contractile activity via its phosphorylation. Phosphorylation triggers actin polymerization in vascular smooth muscle. Implicated in cytokinesis, receptor capping, and cell locomotion.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "MYL12B"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2344 | Hi-Affi™ Recombinant Rabbit Anti-MYL12B Monoclonal Antibody (DS2344AB) | WB, IHC, ICC, IP | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0923-FY119 | Recombinant Mouse Anti-MYL12B Antibody (VS-0923-FY119) | ELISA, WB | Mouse IgG2b |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1419 | Anti-MYL12B Immunohistochemistry Kit | IHC | |
| VS-0525-XY4639 | Anti-Human MYL12B Immunohistochemistry Kit | IHC |
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