Rabbit Anti-NDUFAF4 Recombinant Antibody (clone R04-6A6)
CAT#: VS3-FY1788
This R04-6A6 recombinant monoclonal antibody could potentially be used to target human NDUFAF4. The antibody is recommended for detection of NDUFAF4 protein by WB, ICC/IF, IP. Applications Tested: The NDUFAF4 antibody has been tested by western blot analysis of NDUFAF4 in K562, Hela lysates.
Gene Expression
Tested Data
Subcellular Location
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to mitochondria.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/46560/if_selected.jpg
Normal Tissue
Figure 2 IHC staining of human prostate
Immunohistochemical staining of human prostate shows moderate membranous and cytoplasmic positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/46560/105064_A_2_5_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/46560/105064_B_8_5.jpg
Normal Tissue
Figure 4 Colon
Endothelial cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/46560/105064_A_8_3.jpg
Normal Tissue
Figure 5 Liver
Cholangiocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Hepatocytes Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/46560/105064_A_8_4.jpg
Normal Tissue
Figure 6 Kidney
Cells in glomeruli Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Cells in tubules Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/46560/105064_A_7_5.jpg
Normal Tissue
Figure 7 Testis
Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Leydig cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/46560/105064_A_6_6.jpg
Normal Tissue
Figure 8 Lymph node
Germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/46560/105064_A_8_8.jpg
RNA Expression
Figure 9 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000123545-Ndufaf4
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WB
Figure 1 Anti-NDUFAF4 Antibody (VS3-FY1788) in WB.
The NDUFAF4 expression in K562, Hela lysates were detected using the anti-NDUFAF4 by western blot assay.
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Specifications
- Immunogen
- Recombinant human NDUFAF4 protein
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human NDUFAF4
- Species Reactivity
- Human
- Clone
- R04-6A6
- Applications
- Western Blot, Immunofluorescence, Immunoprecipitation
- MW
- The calculated weight of the target protein is approximately 20 kDa, while the observed molecular weight stands at 20 kDa.
- Related Disease
- Mitochondrial Complex I Deficiency, Nuclear Type 15 and Mitochondrial Complex I Deficiency, Nuclear Type 1
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunofluorescence: 1/50-1/200
Immunoprecipitation: 1/20
Target
- Alternative Names
- NADH:Ubiquinone Oxidoreductase Complex Assembly Factor 4; NADH Dehydrogenase (Ubiquinone) 1 Alpha Subcomplex, Assembly Factor 4; NADH Dehydrogenase (Ubiquinone) Complex I, Assembly Factor 4; Hormone-Regulated Proliferation-Associated Protein Of 20 KDa; C6orf66; HRPAP20; NADH Dehydrogenase [Ubiquinone] 1 Alpha Subcomplex Assembly Factor 4
- Gene ID
- 29078
- UniProt ID
- Q9P032
- Sequence Similarities
- Belongs to the NDUFAF4 family.
- Cellular Localization
- Membrane, Mitochondrion
- Post Translation Modifications
- Phosphorylated on serine.
Prolactin stimulate serine phosphorylation ().
(Q9P032-NDUF4_HUMAN)
Ubiquitination at Lys51, Lys60, Lys66, Lys91, Lys97, and Lys107 (NX_Q9P032 [NX_Q9P032-1])
Modification sites at PhosphoSitePlus (Q9P032)
Glycosylation from GlyGen (Q9P032) 1 site, 1 O-linked glycan (1 site)
- Protein Refseq
- NP_054884.1
- Function
- Involved in the assembly of mitochondrial NADH:ubiquinone oxidoreductase complex (complex I).
May be involved in cell proliferation and survival of hormone-dependent tumor cells. May be a regulator of breast tumor cell invasion.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "Ndufaf4"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1473 | Anti-NDUFAF4 Immunohistochemistry Kit | IHC |
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