Mouse Anti-NELFA Recombinant Antibody (clone 6B11H8) (CAT#: VS7-0425-WR1126)
This product is a mouse IgG2b antibody that binds to human NELFA. The antibody 6B11H8 recognizes specific amino acid domain of human WHSC2 (AA: 280-511). It can be used for WB, IHC, ICC, and FC assays. It cross reacts with rat.
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Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line CACO-2
(Immunofluorescent staining of human cell line CACO-2 shows localization to nucleoplasm & nuclear bodies.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/39858/807_E12_4_selected.jpg
(Immunofluorescent staining of human cell line CACO-2 shows localization to nucleoplasm & nuclear bodies.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/39858/807_E12_4_selected.jpg
Figure 2 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/43931/736_D2_1_selected.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/43931/736_D2_1_selected.jpg
Figure 3 Lymph node
(Germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: nuclear Non-germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/9960/33523_A_9_8.jpg
(Germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: nuclear Non-germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/9960/33523_A_9_8.jpg
Figure 4 Cerebral cortex
(Endothelial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear Glial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: nuclear Neuronal cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/9960/33523_B_8_5.jpg
(Endothelial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear Glial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: nuclear Neuronal cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/9960/33523_B_8_5.jpg
Figure 5 Colon
(Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Nuclear Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/9960/33523_A_8_3.jpg
(Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Nuclear Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/9960/33523_A_8_3.jpg
Figure 6 Kidney
(Cells in glomeruli Staining: Medium Intensity: Strong Quantity: <25% Collecting ducts Staining: High Intensity: Strong Quantity: 75%-25% Distal tubules Staining: High Intensity: Strong Quantity: 75%-25%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/9960/33523_A_9_5.jpg
(Cells in glomeruli Staining: Medium Intensity: Strong Quantity: <25% Collecting ducts Staining: High Intensity: Strong Quantity: 75%-25% Distal tubules Staining: High Intensity: Strong Quantity: 75%-25%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/9960/33523_A_9_5.jpg
Figure 7 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000185049-NELFA
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000185049-NELFA
Specifications
- Immunogen
- Recombinant human WHSC2 (AA: 280-511) fragment expressed in E. Coli.
- Host Species
- Mouse
- Type
- Mouse IgG2b
- Specificity
- Human NELFA
- Species Reactivity
- Human, Rat
- Clone
- 6B11H8
- Molecule Class
- Negative Elongation Factor A (Nelf-A)
- Applications
- WB, IHC, ICC, FC
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purity
- >95% as determined by SDS-PAGE and SEC-HPLC analysis
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- 0.05% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- Western Blot: 1:500 - 1:2000
Immunohistochemistry-Paraffin: 1:200 - 1:1000
Immunocytochemistry: 1:200 - 1:1000
Flow Cytometry: 1:200 - 1:400
Enzyme-linked Immunosorbent Assay: 1:10000
Target
- Alternative Names
- Negative Elongation Factor Complex Member A; Wolf-Hirschhorn Syndrome Candidate 2 Protein; NELF-A; WHSC2; Wolf-Hirschhorn Syndrome Candidate 2; Negative Elongation Factor A; P/OKcl.15
- Gene ID
- 7469
- UniProt ID
- Q9H3P2
- Long Name
- Negative Elongation Factor Complex Member A
- Cellular Localization
- Nucleus
- Post Translation Modifications
- Phosphoprotein
- Protein Refseq
- NP_005654.4
- Function
- Repressor
Recommended Products
Isotype Control
Secondary Antibody
Reconstitution Buffer
Recommended Dilution Buffer
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Chicken IgY Antibody Products
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0388MZ | Chicken Anti-NELFA (ab1) Polyclonal IgY | WB | Chicken antibody |
| BRD-0389MZ | Chicken Anti-NELFA (ab2) Polyclonal IgY | WB | Chicken antibody |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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