Rabbit Anti-NFKBIE Recombinant Antibody (clone R05-1B2)
CAT#: VS3-FY2013
This marvelous rabbit recombinant monoclonal antibody that is purified by affinity chromatography. It demonstrates binding to human NFKBIE. It is suitable for use in a variety of assays, including WB, IP. This R05-1B2 antibody is validated for western blot analysis of IKB epsilon in Hela, Ramos lysates.
Gene Expression
Tested Data
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm, nucleoli fibrillar center & cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5941/29_B5_1_selected.jpg
Normal Tissue
Figure 2 Testis
Elongated or late spermatids Staining: Medium Intensity: Moderate Quantity:>75% Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Pachytene spermatocytes Staining: Medium Intensity: Moderate Quantity:>75% Round or early spermatids Staining: Medium Intensity: Moderate Quantity:>75% Sertoli cells Staining: Medium Intensity: Moderate Quantity:>75% Spermatogonia cells Staining: High Intensity: Strong
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5941/17393_A_6_6.jpg
Normal Tissue
Figure 3 Bone marrow
Hematopoietic cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5941/17393_B_5_4.jpg
Normal Tissue
Figure 4 Lymph node
Germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5941/17393_A_8_8.jpg
Normal Tissue
Figure 5 Colon
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5941/17393_A_8_3.jpg
Normal Tissue
Figure 6 Liver
Cholangiocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Hepatocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5941/17393_A_8_4.jpg
Normal Tissue
Figure 7 Kidney
Cells in glomeruli Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5941/17393_A_8_5.jpg
RNA Expression
Figure 8 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000146232-NFKBIE
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WB
Figure 1 Anti-NFKBIE Antibody (VS3-FY2013) in WB.
The IKB epsilon expression in Hela, Ramos lysates were detected using the anti-IKB epsilon by western blot assay.
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Specifications
- Immunogen
- Synthetic peptide of human IKB epsilon
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human NFKBIE
- Species Reactivity
- Human
- Clone
- R05-1B2
- Applications
- Western Blot, Immunoprecipitation
- MW
- The calculated weight of the target protein is approximately 53 kDa, while the observed molecular weight stands at 48 kDa.
- Related Disease
- St2 Diffuse Large B-Cell Lymphoma and Fetal Encasement Syndrome
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunoprecipitation: 1/20
Target
- Alternative Names
- NFKB Inhibitor Epsilon; Nuclear Factor Of Kappa Light Polypeptide Gene Enhancer In B-Cells Inhibitor, Epsilon; NF-Kappa-B Inhibitor Epsilon; I-Kappa-B-Epsilon; IkappaBepsilon; NF-Kappa-BIE; IkB-Epsilon; IkB-E; IKBE
- Gene ID
- 4794
- UniProt ID
- O00221
- Sequence Similarities
- Belongs to the NF-kappa-B inhibitor family.
- Cellular Localization
- Cytoplasm
- Post Translation Modifications
- Serine phosphorylated; followed by proteasome-dependent degradation. (O00221-IKBE_HUMAN)
Modification sites at PhosphoSitePlus (O00221)
Modification sites at neXtProt (NX_O00221)
- Protein Refseq
- NP_004547.3
- Function
- Inhibits NF-kappa-B by complexing with and trapping it in the cytoplasm. Inhibits DNA-binding of NF-kappa-B p50-p65 and p50-c-Rel complexes.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "NFKBIE"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2437 | Hi-Affi™ Recombinant Rabbit Anti-NFKBIE Monoclonal Antibody (DS2437AB) | WB, IHC, ICC, IP | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-0824-CN | Recombinant Rabbit Anti-NFKBIE Monoclonal Antibody (JM62-63) | WB, IHC, IP | Rabbit IgG |
| VS3-WK602 | Rabbit Anti-NFKBIE Recombinant Antibody (clone JM62-63) | WB, ELISA | Rabbit IgG |
| VS13-YC803 | Rabbit Anti-NFKBIE Recombinant Antibody (VS13-YC803) | WB, IP | Rabbit IgG |
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