Mouse Anti-NHP AK1 Recombinant Antibody (clone OTI19D1)
CAT#: VS-1024-XY9
Designed for high specificity, this mouse-derived monoclonal antibody targets AK1 and exhibits reactivity with both human and non-human primates. The use of a full-length human recombinant AK1 protein as the immunogen enhances its precision. With proven efficacy in Western blot (WB), immunofluorescence (IF), and flow cytometry (FC), this antibody offers versatile utility across experimental settings. Its comprehensive validation makes it an essential tool for researchers exploring AK1 function and expression.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/6456/if_selected.jpg
Normal Tissue
Figure 2 Skeletal muscle
High expression
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/6456/170119_B_2_6_val_selected.jpg
Normal Tissue
Figure 3 Fallopian tube
Ciliated cells
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/9893/25253_A_8_7.jpg
Normal Tissue
Figure 4 Cerebral cortex
Neuronal cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/9893/25253_B_8_5.jpg
Normal Tissue
Figure 5 Colon
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/9893/25253_A_7_3.jpg
Normal Tissue
Figure 6 Testis
Elongated or late spermatids Staining: Low Intensity: Weak Quantity: 75%-25% Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Spermatogonia cells Staining: Medium Intensity: Moderate
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/9893/25253_A_6_6.jpg
RNA Expression
Figure 7 RNA cell line category: Cell line enhanced (U-2197)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000106992-AK1
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Specifications
- Immunogen
- Full length human recombinant protein of AK1
- Host Species
- Mouse
- Type
- Mouse IgG2b
- Specificity
- AK1
- Species Reactivity
- Human, Non-human primate
- Clone
- OTI19D1
- Applications
- WB, IF, FC
Product Property
- Purification
- Affinity Chromatography
- Purity
- >95%, determined by SDS-PAGE
- Format
- Liquid
- Buffer
- PBS, pH 7.4, with 1% BSA, 50% glycerol
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Applications
- Application Notes
- WB: 1:800-1:20, IF: 1:50, FC: 1:100
Target
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Recommended Dilution Buffer
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
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| MOB-2238z | Mouse Anti-AK1 Recombinant Antibody (clone 13B1) | WB, FC, ICC, IF | Mouse IgG1 |
| VS3-CJ218 | Mouse Anti-AK1 Recombinant Antibody (VS3-CJ218) | WB |
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| MOR-0115 | Hi-Affi™ Rabbit Anti-AK1 Recombinant Antibody (clone DS115AB) | WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
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| VS-0724-YC1314 | AbPlus™ Anti-AK1 Magnetic Beads (VS-0724-YC1314) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY75 | Anti-AK1 Immunohistochemistry Kit | IHC |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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