Mouse Anti-NHP DDT Recombinant Antibody (clone OTI1H3)
CAT#: VS-1024-XY158
This mouse-derived antibody targets DDT, with notable reactivity to human and non-human primate tissues. The antibody is generated against a full-length recombinant human DDT protein, ensuring precise recognition. It is well-suited for Western blot (WB) and immunohistochemistry (IHC) applications, offering reliable performance in detecting DDT across relevant biological samples. Ideal for both research and clinical settings, this antibody provides essential insights into DDT expression and function.
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to mitochondria.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/49871/if_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IHC staining of human liver
Immunohistochemical staining of human liver shows strong cytoplasmic and nuclear positivity in hepatocytes.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/49871/ihc_selected.jpg
Subcellular Location and Protein Expression
Figure 3 IF staining of human cell line HEK293
Immunofluorescent staining of human cell line HEK293 shows localization to mitochondria.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/49871/805_A9_1_blue_red_green.jpg
Normal Tissue
Figure 4 Colon
Glandular cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/49871/110715_A_9_3.jpg
Normal Tissue
Figure 5 Liver
Hepatocytes
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/49871/110715_A_7_4.jpg
Normal Tissue
Figure 6 Kidney
Cells in tubules
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/49871/110715_A_8_5.jpg
Normal Tissue
Figure 7 Testis
Elongated or late spermatids
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Leydig cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Preleptotene spermatocytes
Staining:Medium
Intensity: Strong
Quantity: <25%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/49871/110715_A_5_6.jpg
RNA Expression
Figure 8 RNA cell line category: Cell line enhanced (HEK93)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000099977-DDT
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Specifications
- Immunogen
- Full length human recombinant protein of DDT
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- DDT
- Species Reactivity
- Human, Non-human primate
- Clone
- OTI1H3
- Applications
- WB, IHC
Product Property
- Purification
- Protein A purified
- Purity
- >95%, determined by SDS-PAGE
- Format
- Liquid
- Buffer
- PBS, pH 7.4, with 1% BSA, 50% glycerol
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Applications
- Application Notes
- WB: 1:200-1:8,000, IHC: 1:200
Target
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Recommended Dilution Buffer
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "DDT"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NAB-754-sdAb | Recombinant Anti-Human DDT VHH Single Domain Antibody | IHC, ICC, IP, ChiP, Neut | Llama VHH |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1360MZ | Recombinant Mouse Anti-Human DDT Antibody (clone 1D5) | WB | Mouse antibody |
| FAMAB-0084YC | Mouse Anti-DDT Recombinant Antibody (clone 3D7) | ELISA | Mouse IgG1, κ |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| FAMAB-0084-YC-S(P) | Mouse Anti-DDT Recombinant Antibody (clone 3D7); scFv Fragment | ELISA | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| FAMAB-0084-YC-F(E) | Mouse Anti-DDT Recombinant Antibody (clone 3D7); Fab Fragment | ELISA | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY640 | Anti-DDT Immunohistochemistry Kit | IHC |
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