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    Mouse Anti-NHP EP300 Recombinant Antibody (clone RW128) (CAT#: VS-1024-XY360)

    Focusing on the pivotal role of EP300, this mouse-derived antibody displays impressive reactivity with human, mustelid, mouse, non-human primate, and rat samples. Generated against a fusion protein immunogen, it is adept at facilitating a range of applications, including Western Blotting (WB), Immunocytochemistry (ICC), Flow Cytometry (FC), and Immunoprecipitation (IP). This versatility makes it an indispensable asset for researchers aiming to dissect the multifaceted roles of EP300 in transcriptional regulation and cellular signaling pathways. Employ this antibody to deepen your investigations into the mechanisms governing gene expression and cellular function.

    Specific Inquiry
    • Size:

    • Conjugation:

    • Endotoxin:

    • Purity:

    • Formats:

    • Isotype Switching:

    • Fc Engineering:

    • Modalities:

    Custom Production

    We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.

    Sequences:
    Isotype :
    Purity :
    Endotoxin :
    Quantity :
    Conjugation :
    Fc Engineering :
    Modalities :
    Other Requirements:
    We require custom production
    • Gene Expression
    • Datasheet
    • MSDS
    • COA
    Subcellular Location and Protein Expression
    Figure 1 IF staining of human cell line U-2 OS
    Figure 1 IF staining of human cell line U-2 OS
    (Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4112/if_selected.jpg
    Figure 2 IHC staining of human colon
    Figure 2 IHC staining of human colon
    (Immunohistochemical staining of human colon shows strong nuclear and cytoplasmic positivity in glandular cells.)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3128/ihc_selected.jpg
    Figure 3 IHC staining of human lymph node
    Figure 3 IHC staining of human lymph node
    (Immunohistochemical staining of human lymph node shows strong nuclear positivity in reaction center cells.)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4112/ihc_selected.jpg
    Figure 4 IHC staining of human urinary bladder
    Figure 4 IHC staining of human urinary bladder
    (Immunohistochemical staining of human urinary bladder shows moderate nuclear positivity in urothelial cells.)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/146/ihc_selected.jpg
    Figure 5 IF staining of human cell line A-431
    Figure 5 IF staining of human cell line A-431
    (Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3128/14_A2_1_red_green.jpg
    Figure 6 IF staining of human cell line U-251 MG
    Figure 6 IF staining of human cell line U-251 MG
    (Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3128/16_A2_1_red_green.jpg
    Normal Tissue
    Figure 6 Cerebral cortex
    Figure 6 Cerebral cortex
    (Endothelial cells
    Staining:Medium
    Intensity: Moderate
    Quantity: 75%-25%
    Location: Cytoplasmic/membranous nuclear
    Neuronal cells
    Staining:High
    Intensity: Strong
    Quantity:>75%
    Location: Cytoplasmic/membranous nuclear)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3128/8834_B_9_5.jpg
    Figure 7 Colon
    Figure 7 Colon
    (Glandular cells
    Staining:High
    Intensity: Strong
    Quantity:>75%
    Location: Cytoplasmic/membranous nuclear
    Peripheral nerve/ganglion
    Staining:Medium
    Intensity: Moderate
    Quantity:>75%
    Location: Nuclear)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3128/8834_A_7_3.jpg
    Figure 8 Liver
    Figure 8 Liver
    (Cholangiocytes
    Staining:Medium
    Intensity: Moderate
    Quantity: 75%-25%
    Location: Nuclear)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3128/8834_A_8_4.jpg
    Figure 9 Kidney
    Figure 9 Kidney
    (Cells in glomeruli
    Staining:Medium
    Intensity: Moderate
    Quantity: 75%-25%
    Location: Nuclear
    Cells in tubules
    Staining:Medium
    Intensity: Moderate
    Quantity:>75%
    Location: Cytoplasmic/membranous nuclear)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3128/8834_A_8_5.jpg
    Figure 10 Urinary bladder
    Figure 10 Urinary bladder
    (Urothelial cells
    Staining:Medium
    Intensity: Moderate
    Quantity:>75%
    Location: Cytoplasmic/membranous nuclear)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3128/8834_A_6_5.jpg
    Figure 11 Testis
    Figure 11 Testis
    (Elongated or late spermatids
    Staining:High
    Intensity: Strong
    Quantity: 75%-25%
    Sertoli cells
    Staining:Medium
    Intensity: Moderate
    Quantity:>75%)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3128/8834_A_5_6.jpg
    Figure 12 Lymph node
    Figure 12 Lymph node
    (Germinal center cells
    Staining:Medium
    Intensity: Moderate
    Quantity:>75%
    Location: Cytoplasmic/membranous nuclear)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3128/8834_A_8_8.jpg
    RNA Expression
    Figure 13 RNA cell line category: Low cell line specificity
    Figure 13 RNA cell line category: Low cell line specificity
    (Cell lines ordered by descending RNA expression order.)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000100393-EP300

    Specifications

    • Immunogen
    • Fusion protein
    • Host Species
    • Mouse
    • Type
    • Mouse IgG1, kappa
    • Specificity
    • EP300
    • Species Reactivity
    • Human, Mustelid, Mouse, Non-human primate, Rat
    • Clone
    • RW128
    • Applications
    • WB, ICC, FC, IP

    Product Property

    • Purification
    • Protein A purified
    • Purity
    • >95%, determined by SDS-PAGE
    • Format
    • Liquid
    • Buffer
    • PBS, pH 7.4
    • Storage
    • Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

    Applications

    • Application Notes
    • WB: 1:250-1:500, ICC: 2-5 µg/mL, FC: 1 µg/mL, IP: 1:10-1:500

    Target

    • Alternative Names
    • p300; KAT3B; MKHK2; RSTS2

    Recommended Products

    Recommended Dilution Buffer

    Isotype Control

    Secondary Antibody

    Product Notes

    This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

    • Increased sensitivity
    • Confirmed specificity
    • High repeatability
    • Excellent batch-to-batch consistency
    • Sustainable supply
    • Animal-free production

    See more details about Hi-Affi™ recombinant antibody benefits.

    Downloads

    Download resources about recombinant antibody development and antibody engineering to boost your research.

    See other products for "EP300"

    Select a product category from the dropdown menu below to view related products.
    Please select product type
    IgG Antibody Products IHC Kit and Antibody Products: Precision Tools for Immunohistochemistry
    CAT Product Name Application Type
    DrMAB-318 Human Anti-EP300 Recombinant Antibody (clone D429) ELISA, IP-MS Human IgG
    MOB-2336CT Recombinant Mouse anti-Human EP300 Monoclonal antibody (Z84) IF, IHC-P, IP, WB
    ZG-0338C Mouse Anti-EP300 Recombinant Antibody (ZG-0338C) WB, ELISA Mouse IgG
    VS3-XY624 Mouse Anti-EP300 Recombinant Antibody (clone 7D8A6) ELISA, WB Mouse IgG1
    VS7-HM863 Mouse Anti-EP300 Recombinant Antibody (clone CBL228HM) WB, ELISA Mouse IgG

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    For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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