Mouse Anti-EP300 Recombinant Antibody (clone NM11)
CAT#: VS-0725-FY164
This recombinant antibody, clone NM11, targets full-length p300 protein purified from human 293 cells. It is suitable for Chromatin Immunoprecipitation (ChIP), Chromatin Immunoprecipitation-Sequencing (ChIP-Seq), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), and Western Blot (WB). EP300 is a crucial transcriptional co-activator that functions as a histone acetyltransferase, regulating transcription via chromatin remodeling and playing a vital role in cell proliferation and differentiation. This antibody is a valuable reagent for research into transcriptional regulation and cell biology.
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4112/if_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IHC staining of human colon
Immunohistochemical staining of human colon shows strong nuclear and cytoplasmic positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3128/ihc_selected.jpg
Subcellular Location and Protein Expression
Figure 3 IHC staining of human lymph node
Immunohistochemical staining of human lymph node shows strong nuclear positivity in reaction center cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4112/ihc_selected.jpg
Subcellular Location and Protein Expression
Figure 4 IHC staining of human urinary bladder
Immunohistochemical staining of human urinary bladder shows moderate nuclear positivity in urothelial cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/146/ihc_selected.jpg
Subcellular Location and Protein Expression
Figure 5 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3128/14_A2_1_red_green.jpg
Subcellular Location and Protein Expression
Figure 6 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3128/16_A2_1_red_green.jpg
Normal Tissue
Figure 6 Cerebral cortex
Endothelial cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear
Neuronal cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3128/8834_B_9_5.jpg
Normal Tissue
Figure 7 Colon
Glandular cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
Peripheral nerve/ganglion
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3128/8834_A_7_3.jpg
Normal Tissue
Figure 8 Liver
Cholangiocytes
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3128/8834_A_8_4.jpg
Normal Tissue
Figure 9 Kidney
Cells in glomeruli
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear
Cells in tubules
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3128/8834_A_8_5.jpg
Normal Tissue
Figure 10 Urinary bladder
Urothelial cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3128/8834_A_6_5.jpg
Normal Tissue
Figure 11 Testis
Elongated or late spermatids
Staining:High
Intensity: Strong
Quantity: 75%-25%
Sertoli cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3128/8834_A_5_6.jpg
Normal Tissue
Figure 12 Lymph node
Germinal center cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3128/8834_A_8_8.jpg
RNA Expression
Figure 13 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000100393-EP300
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Specifications
- Immunogen
- Full-length p300 protein, which was purified from human 293 cells.
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Human EP300
- Species Reactivity
- Human
- Clone
- NM11
- Applications
- Chromatin Immunoprecipitation, Chromatin Immunoprecipitation-Sequencing, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Western Blot
- Related Disease
- Rubinstein-Taybi Syndrome
Product Property
- Purification
- Protein A purified
- Molecular Weight
- The molecular weight of the antigen is 300 kDa.
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS, 30% glycerol
- Preservative
- 0.035% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Chromatin Immunoprecipitation: 5 µg per Chromatin Immunoprecipitation
Chromatin Immunoprecipitation-Sequencing: 5 µg each
Immunocytochemistry/Immunofluorescence: 0.5 µg/mL.
Target
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "EP300"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| DrMAB-318 | Human Anti-EP300 Recombinant Antibody (clone D429) | ELISA, IP-MS | Human IgG |
| MOB-2336CT | Recombinant Mouse anti-Human EP300 Monoclonal antibody (Z84) | IF, IHC-P, IP, WB | |
| ZG-0338C | Mouse Anti-EP300 Recombinant Antibody (ZG-0338C) | WB, ELISA | Mouse IgG |
| VS3-XY624 | Mouse Anti-EP300 Recombinant Antibody (clone 7D8A6) | ELISA, WB | Mouse IgG1 |
| VS7-HM863 | Mouse Anti-EP300 Recombinant Antibody (clone CBL228HM) | WB, ELISA | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-1024-XY360 | Mouse Anti-NHP EP300 Recombinant Antibody (clone RW128) | WB, ICC, FC, IP | Mouse IgG1, kappa |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY769 | Anti-EP300 Immunohistochemistry Kit | IHC |
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