Mouse Anti-NHP NEFM Recombinant Antibody (clone RNF 406) (CAT#: VS-1024-XY339)
This antibody, engineered to recognize NEFM, exhibits extensive reactivity across a diverse array of species, including bovine, guinea pig, human, non-human primate, rabbit, and rat. Derived from neurofilament preparation of calf brain tissue, it is specifically designed to provide reliable performance in Western Blotting (WB) and Immunohistochemistry (IHC) applications. Its robust specificity and sensitivity make it an indispensable asset for researchers investigating neurofilament dynamics and related neurological conditions. Utilize this antibody to deepen your understanding of neuronal architecture and function across various models.
Specific Inquiry
Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line HEK 293
(Immunofluorescent staining of human cell line HEK 293 shows localization to intermediate filaments.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/22845/1729_F9_1_selected.jpg
(Immunofluorescent staining of human cell line HEK 293 shows localization to intermediate filaments.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/22845/1729_F9_1_selected.jpg
Figure 2 High expression in cerebral cortex
(Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/22845/53155_B_8_5.jpg
(Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/22845/53155_B_8_5.jpg
Figure 3 Colon
(Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/22845/53155_A_7_3.jpg
(Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/22845/53155_A_7_3.jpg
Figure 4 RNA cell line category: Cell line enriched (HEK93)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000104722-NEFM
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000104722-NEFM
Specifications
- Immunogen
- Neurofilament preparation of calf brain tissue
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- NEFM
- Species Reactivity
- Bovine, Guinea pig, Human, Non-human primate, Rabbit, Rat
- Clone
- RNF 406
- Applications
- WB, IHC
Product Property
- Purification
- Protein A purified
- Purity
- >95%, determined by SDS-PAGE
- Format
- Liquid
- Buffer
- PBS, pH 7.4
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Applications
- Application Notes
- WB: 1:100-1:500, IHC: 1:50-1:100
Target
- Alternative Names
- Neurofilament Medium; Neurofilament; Medium Polypeptide 150kDa; Neurofilament Triplet M Protein; 160 KDa Neurofilament Protein; Neurofilament 3; NEF3;
- Gene ID
- 4741
- UniProt ID
- P07197
Recommended Products
Recommended Dilution Buffer
Isotype Control
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
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IgG Antibody Products
Chicken IgY Antibody Products
Rabbit Monoclonal Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2305z | Mouse Anti-NEFM Recombinant Antibody (clone 39A11) | WB, ELISA, FC, ICC, IHC | Mouse IgG1 |
| MOB-2459CT | Recombinant Mouse anti-Cow NEF3 Monoclonal antibody (3H11) | IP, IHC-P, ELISA, WB | |
| MRO-1073-CN | Recombinant Rabbit Anti-NEFM Monoclonal Antibody (JM11-20) | WB, IF, IHC, IP | Rabbit IgG |
| ZG-1090F | Mouse Anti-NEFM Recombinant Antibody (clone RNF403) | IHC-Fr, IHC-P, WB, IF | Mouse IgG1 |
| VS-0223-WK52 | Rabbit Anti-NEFM Recombinant Antibody (VS-0223-WK52) | WB, ICC, IHC, IHC-P | Rabbit IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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