Mouse Anti-NHP NQO2 Recombinant Antibody (clone OTI3G4)
CAT#: VS-1024-XY348
Targeting the NQO2 protein, this antibody is engineered to recognize a broad spectrum of species, including dog, human, mouse, and non-human primate. The immunogen consists of the full-length human recombinant NQO2 protein, ensuring high specificity and affinity. With proven applications in Western Blotting (WB) and Immunohistochemistry (IHC), this mouse-derived antibody is essential for investigations into oxidative stress and its implications in various diseases. Leverage this powerful tool to deepen your understanding of NQO2's role in cellular defense mechanisms.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/21332/if_selected.jpg
Normal Tissue
Figure 2 Testis
Leydig cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/21332/49424_A_4_6.jpg
Normal Tissue
Figure 3 Colon
Glandular cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/21332/49424_A_9_3.jpg
Normal Tissue
Figure 4 Liver
Cholangiocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Hepatocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/21332/49424_A_7_4.jpg
Normal Tissue
Figure 5 Kidney
Cells in tubules Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/21332/49424_A_9_5.jpg
RNA Expression
Figure 6 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000124588-NQO2
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Specifications
- Immunogen
- Full length human recombinant protein of NQO2
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- NQO2
- Species Reactivity
- Dog, Human, Mouse, Non-human primate
- Clone
- OTI3G4
- Applications
- WB, IHC
Product Property
- Purification
- Protein A purified
- Purity
- >95%, determined by SDS-PAGE
- Format
- Liquid
- Buffer
- PBS, pH 7.4, with 1% BSA, 50% glycerol
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Applications
- Application Notes
- WB: 1:500-1:20, IHC: 1:200
Target
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Recommended Dilution Buffer
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2486 | Hi-Affi™ Recombinant Rabbit Anti-NQO2 Monoclonal Antibody (DS2486AB) | WB, FC | IgG |
| CAT | Product Name | Application | Type |
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| MRO-1109-CN | Recombinant Rabbit Anti-NQO2 Monoclonal Antibody (CBACN-407) | WB | Rabbit IgG |
| VS3-CJ687 | Rabbit Anti-NQO2 Recombinant Antibody (VS3-CJ687) | WB, FC | Rabbit IgG |
| VS4-CJ59 | Recombinant Rabbit Anti-NQO2 Antibody (clone 8E12) | ELISA, WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC844 | AbPlus™ Anti-NQO2 Magnetic Beads (VS-0724-YC844) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY4936 | Anti-NQO2 Immunohistochemistry Kit | IHC |
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