Rabbit Anti-NHP PARP1 Recombinant Antibody (VS-1024-XY366)
CAT#: VS-1024-XY366
This rabbit-derived antibody targets PARP1, utilizing a synthetic peptide immunogen to ensure precise recognition. It exhibits robust reactivity with human, mouse, non-human primate, and rat samples, making it a versatile asset for diverse research applications. With proven efficacy in Western Blotting (WB), Immunocytochemistry (ICC), and Immunoprecipitation (IP), this antibody facilitates detailed exploration of cellular repair mechanisms and apoptotic pathways. Its exceptional specificity and sensitivity provide researchers with a powerful tool for dissecting the roles of PARP1 in various biological contexts, enhancing our understanding of cellular responses to stress and damage.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3839/if_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/75726/1894_D1_3_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Endothelial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear Glial cells Staining: High Intensity: Strong Quantity:>75% Location: nuclear Neuronal cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/45168/101032_B_7_5.jpg
Normal Tissue
Figure 4 Colon
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Glandular cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Nuclear Peripheral nerve/ganglion Staining: High Intensity: Strong Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/45168/101032_A_9_3.jpg
Normal Tissue
Figure 5 Liver
Cholangiocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Hepatocytes Staining: High Intensity: Strong Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/45168/101032_A_7_4.jpg
Normal Tissue
Figure 6 Kidney
Cells in glomeruli Staining: High Intensity: Strong Quantity: 75%-25% Location: nuclear Cells in tubules Staining: High Intensity: Strong Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/45168/101032_A_9_5.jpg
Normal Tissue
Figure 7 Testis
Pachytene spermatocytes Staining: Medium Intensity: Moderate Quantity:>75% Preleptotene spermatocytes Staining: High Intensity: Strong Quantity:>75% Round or early spermatids Staining: High Intensity: Strong Quantity: 75%-25% Sertoli cells Staining: Medium Intensity: Moderate Quantity:>75% Spermatogonia cells Staining: High Intensity: Strong
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3840/10734_A_4_6.jpg
Normal Tissue
Figure 8 Lymph node
Germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Non-germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/45168/101032_A_7_8.jpg
RNA Expression
Figure 9 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000143799-PARP1
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Specifications
- Immunogen
- Synthetic peptide
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- PARP1
- Species Reactivity
- Human, Mouse, Non-human primate, Rat
- Applications
- WB, ICC, IP
Product Property
- Purification
- Protein A purified
- Purity
- >95%, determined by SDS-PAGE
- Format
- Liquid
- Buffer
- 0.01 M HEPES, pH 7.5, with 0.15 M NaCl, 100 µg/mL BSA, 50% glycerol
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Applications
- Application Notes
- WB: 1:500, ICC: 1:800, IP: 1:200
Target
- Alternative Names
- Poly(ADP-Ribose) Polymerase 1; ADP-Ribosyltransferase (NAD+; Poly (ADP-Ribose) Polymerase); ADP-Ribosyltransferase Diphtheria Toxin-Like 1; Poly (ADP-Ribose) Polymerase Family; Member 1; NAD(+) ADP-Ribosyltransferase 1; Poly[ADP-Ribose] Synthase 1; EC 2.4.2.30; ADPRT 1; PARP-1; ADPRT; ARTD1
- Gene ID
- 142
- UniProt ID
- P09874
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
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- Product Name
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- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "PARP1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2594 | Hi-Affi™ Recombinant Rabbit Anti-PARP1 Monoclonal Antibody (DS2594AB) | FC, ICC, IHC-P, IP, WB | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-1165-CN | Recombinant Rabbit Anti-PARP1 Monoclonal Antibody (CBACN-425) | WB, IF, IHC, FC | Rabbit IgG |
| MRO-1166-CN | Recombinant Mouse Anti-PARP1 Monoclonal Antibody (A0-D11) | WB, IF, IHC, FC | Mouse IgG1 |
| ZG-0499U | Rabbit Anti-PARP1 Recombinant Antibody (clone 7C11) | ELISA, WB, IHC | Rabbit IgG |
| ZG-0500U | Rabbit Anti-PARP1 Recombinant Antibody (clone 8C7) | ELISA, WB, IHC, IF, FC | Rabbit IgG |
| ZG-0501U | Rabbit Anti-PARP1 Recombinant Antibody (clone 5H8) | ELISA, IHC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC1341 | AbPlus™ Anti-PARP1 Magnetic Beads (VS-0724-YC1341) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS13-YC868 | CytoStream™ Rabbit Anti-PARP1 Recombinant Antibody (VS13-YC868) | WB, ICC, IF, IP, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1565 | Anti-PARP1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY5185 | Anti-Mouse PARP1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY5184 | Anti-Human PARP1 Immunohistochemistry Kit | IHC |
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