Mouse Anti-NHP RYR1 Recombinant Antibody (clone 34C)
CAT#: VS-1024-XY460
Designed to recognize RYR1, this exceptional monoclonal antibody displays broad reactivity across diverse species, including amphibians, bovines, fish, humans, mustelids, mice, non-human primates, sheep, rabbits, and rats. Utilizing a partially purified chicken pectoral muscle ryanodine receptor as its immunogen, it ensures high specificity and effective binding. Ideal for applications in Western blotting and immunohistochemistry, this antibody is indispensable for investigating RYR1's role in calcium signaling and muscle physiology. Its robustness and versatility make it a valuable resource for researchers delving into the complexities of RYR1 function across various biological contexts.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line SiHa
Immunofluorescent staining of human cell line SiHa shows localization to cytosol & the Golgi apparatus.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/56416/1183_H4_2_selected.jpg
Normal Tissue
Figure 2 High expression in skeletal muscle
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/56416/140331_B_2_6_val_selected.jpg
Normal Tissue
Figure 3 Low expression in colon
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/56416/140331_A_8_3_val_selected.jpg
Normal Tissue
Figure 4 Skeletal muscle
Myocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/56416/140331_B_1_6.jpg
RNA Expression
Figure 5 RNA cell line category: Cell line enhanced (A549, HaCaT, hTCEpi, RH-30, SiHa, U-2 OS)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000196218-RYR1
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Specifications
- Immunogen
- Partially purified chicken pectoral muscle ryanodine receptor
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- RYR1
- Species Reactivity
- Amphibian, Bovine, Fish, Human, Mustelid, Mouse, Non-human primate, Sheep, Rabbit, Rat
- Clone
- 34C
- Applications
- WB, IHC
Product Property
- Purification
- Protein A purified
- Purity
- >95%, determined by SDS-PAGE
- Format
- Liquid
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Applications
- Application Notes
- WB: 1:5,000, IHC: 1:20
Target
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Recommended Dilution Buffer
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "Clone 34C"
- CAT
- Product Name
See other products for "RYR1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1355z | Mouse Anti-RYR1 Recombinant Antibody (clone 41B1) | ELISA, FC, IF, IHC, WB | Mouse IgG2b |
| MRO-2173-CN | Rabbit Anti-RYR1 Polyclonal Antibody (MRO-2173-CN) | Dot, IHC, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-1924CQ | Recombinant Mouse Anti-RYR1 Antibody (34C) | BL, ICC, IF, IHC, IHC-F, IHC-Fr, IP, WB | IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY6290 | Anti-Mouse RYR1 Immunohistochemistry Kit | IHC |
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