Mouse Anti-NHP SETDB1 Recombinant Antibody (clone 5H6A12)
CAT#: VS-1024-XY474
Focusing on SETDB1, this antibody is crafted from a purified recombinant fragment, ensuring optimal specificity and reactivity with human, mouse, and non-human primate samples. Its applications in Western Blotting (WB), Immunocytochemistry (ICC), and ELISA provide researchers with a comprehensive toolkit for investigating the role of SETDB1 in epigenetic regulation and gene expression. Renowned for its exceptional sensitivity and reliability, this antibody is crucial for advancing our understanding of the mechanisms underlying various biological processes and disease states, ultimately enhancing the exploration of therapeutic strategies.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/58484/if_selected.jpg
Normal Tissue
Figure 2 Cerebral cortex
Glial cells Staining: Medium Intensity: Strong Quantity: <25% Location: Nuclear Neuronal cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear Neuropil Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/18142/43432_B_9_5.jpg
Normal Tissue
Figure 3 Colon
Endothelial cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous Glandular cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear Peripheral nerve/ganglion Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/18142/43432_A_7_3.jpg
Normal Tissue
Figure 4 Kidney
Cells in glomeruli Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous nuclear Cells in tubules Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/18142/43432_A_8_5.jpg
Normal Tissue
Figure 5 Testis
Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Leydig cells Staining: Medium Intensity: Strong Quantity: <25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/18142/43432_A_6_6.jpg
Normal Tissue
Figure 6 Lymph node
Germinal center cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous nuclear Non-germinal center cells Staining: Medium Intensity: Strong Quantity: <25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/18142/43432_A_9_8.jpg
RNA Expression
Figure 7 RNA cell line category: Cell line enriched (U-698)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000143379-SETDB1
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Specifications
- Immunogen
- Purified recombinant fragment
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- SETDB1
- Species Reactivity
- Human, Mouse, Non-human primate
- Clone
- 5H6A12
- Applications
- WB, ICC, ELISA
Product Property
- Purification
- Protein A purified
- Purity
- >95%, determined by SDS-PAGE
- Format
- Liquid
- Buffer
- PBS, pH 7.4
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Applications
- Application Notes
- WB: 1:500-1:1000, ICC: 1:200-1:800, ELISA: 1:8000
Target
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
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COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Secondary Antibody
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See other products for "SETDB1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1037CT | Recombinant Mouse anti-Human SETDB1 Monoclonal antibody (4A3) | ELISA, IHC-P, WB | |
| ZG-0322J | Mouse Anti-SETDB1 Recombinant Antibody (ZG-0322J) | WB, ELISA | Mouse IgG |
| ZG-0323J | Mouse Anti-SETDB1 Recombinant Antibody (ZG-0323J) | WB, IF, ELISA | Mouse IgG |
| VS3-QX1005 | Mouse Anti-SETDB1 Recombinant Antibody (clone 1E7-F3-C6) | WB, IHC, ICC, FC, ELISA | Mouse IgG1 |
| VS3-XY1417 | Mouse Anti-SETDB1 Recombinant Antibody (clone 5H6D4) | ELISA, WB | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY2041 | Anti-SETDB1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY6515 | Anti-Human SETDB1 Immunohistochemistry Kit | IHC |
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