Mouse Anti-NHP TBP Recombinant Antibody (clone 1TBP18)
CAT#: VS-1024-XY501
Engineered to recognize TBP, this monoclonal antibody exhibits robust reactivity with diverse species, including dog, human, mouse, non-human primate, and rat. Its applications in Western blot, immunofluorescence, and flow cytometry underscore its versatility in research settings. By utilizing a highly specific approach, it facilitates the study of TBP's pivotal role in transcriptional regulation and gene expression dynamics. With its reliability and sensitivity, this antibody serves as an essential reagent for exploring the intricate mechanisms underlying cellular function and disease states.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line MCF7
Immunofluorescent staining of human cell line MCF7 shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/49805/if_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/9442/if_selected.jpg
Normal Tissue
Figure 3 Urinary bladder
Urothelial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/49805/146472_A_5_5.jpg
RNA Expression
Figure 4 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000112592-TBP
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Specifications
- Immunogen
- N-terminal domain of the human TATA-binding protein
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- TBP
- Species Reactivity
- Dog, Human, Mouse, Non-human primate, Rat
- Clone
- 1TBP18
- Applications
- WB, IF, FC
Product Property
- Purification
- Protein A purified
- Purity
- >95%, determined by SDS-PAGE
- Format
- Liquid
- Buffer
- PBS, pH 7.4, with 30% glycerol, 1 mg/mL BSA
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Applications
- Application Notes
- WB: 1:800, IF: 1-20 µg/mL, FC: 1-20 µg/mL
Target
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Recommended Dilution Buffer
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
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| CAT | Product Name | Application | Type |
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