Rabbit Anti-NKX2-1 Recombinant Antibody (clone 6C11) (CAT#: ZG-0487U)
Rabbit Anti-NKX2-1 Antibody (clone 6C11) displayed high specificity against the NKX2-1 protein of Human.
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WB
Figure 1 Rabbit Anti-NKX2-1 Antibody (ZG-0487U) in WB
Western Blot
Positive WB detected in: HepG2 whole cell lysate, U251 whole cell lysate, U87 whole cell lysate
All lanes: TTF1 antibody at 1:2000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 39 kDa
Observed band size: 39 kDa
IHC
Figure 2 Rabbit Anti-NKX2-1 Antibody (ZG-0487U) in IHC
IHC image of ZG-0487U diluted at 1:100 and staining in paraffin-embedded human lung cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
FC
Figure 3 Rabbit Anti-NKX2-1 Antibody (ZG-0487U) in FC
Overlay histogram showing A549 cells stained with ZG-0487U (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then incubated in 10% normal goat serum to block non-specific protein-protein interactions followedby the antibody (1µg/1*106cells) for 1 h at 4°C.The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 30min at 4°C. Control antibody (green line) was Rabbit IgG (1µg/1*106cells) used under the same conditions. Acquisition of>10, 000 events was performed.
Specifications
- Immunogen
- A synthesized peptide derived from human TTF1
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human NKX2-1
- Species Reactivity
- Human
- Clone
- 6C11
- Applications
- ELISA, WB, IHC, FC
- Conjugate
- Unconjugated
- Related Disease
- Choreoathetosis And Congenital Hypothyroidism With Or Without Pulmonary Dysfunction and Chorea, Benign Hereditary.
Product Property
- Purification
- Affinity chromatography purified
- Buffer
- PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
- Preservative
- 0.02% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Enzyme-linked Immunosorbent Assay, Western Blot, Immunohistochemistry, Flow Cytometry. Specific conditions should be optimized by the end user.
WB:1:500-1:5000, IHC:1:50-1:200, FC:1:20-1:200
Target
- Alternative Names
- Homeobox protein Nkx-2.1; Homeobox protein NK-2 homolog A; Thyroid nuclear factor 1; Thyroid transcription factor 1; TTF-1; Thyroid-specific enhancer-binding protein; T/EBP; NKX2-1; NKX2A TITF1 TTF1
- Gene ID
- 7080
- UniProt ID
- P43699
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Rabbit Monoclonal Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1310z | Mouse Anti-NKX2-1 Recombinant Antibody (clone 11B10) | IF, IHC, WB | Mouse IgG2b |
| MOB-0342MZ | Recombinant Mouse Anti-Human NK2 Homeobox 1 Antibody (clone 9H8G4/2) | IHC | Mouse antibody |
| ZG-0122C | Mouse Anti-NKX2-1 Recombinant Antibody (clone ABT-TTF1) | IHC-P, IF | Mouse IgG |
| ZG-0123C | Mouse Anti-NKX2-1 Recombinant Antibody (clone PT2256) | IHC-P, IF | Mouse IgG |
| VS13-YC811 | Rabbit Anti-NKX2-1 Recombinant Antibody (VS13-YC811) | WB, ICC, IF | Rabbit IgG |
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For Research Use Only. Not For Clinical Use.
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