Rabbit Anti-NKX2-1 Recombinant Antibody (clone R09-1H7) (CAT#: VS3-FY1918)
This R09-1H7 recombinant monoclonal antibody is specifically designed to target human NKX2-1. It cross-reacts with hamster. It is suitable for use in a variety of assays, including WB, ICC/IF. The antibody has the positive detection of thyroid transcription factor 1 protein by western blot analysis of TSG101 in K562 lysates and immunocytochemistry analysis of thyroid transcription factor 1 (green) in K562 using thyroid transcription factor 1 antibody.
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WB
Figure 1 Anti-NKX2-1 Antibody (VS3-FY1918) in WB.
The TTF1 expression in K562 lysates were detected using the anti-TTF1 by western blot assay.
ICC
Figure 2 Anti-NKX2-1 Antibody (VS3-FY1918) in ICC.
Immunocytochemistry staining of Thyroid Transcription Factor 1 (green) in K562 using the anti-Thyroid Transcription Factor 1 antibody. Nuclear DNA was labeled with DAPI (shown in blue).
Specifications
- Immunogen
- Synthetic peptide of human TTF1
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Hamster NKX2-1
- Species Reactivity
- Human, Hamster
- Clone
- R09-1H7
- Applications
- Western Blot, Immunofluorescence
- MW
- The calculated weight of the target protein is approximately 39 kDa, while the observed molecular weight stands at 39 kDa.
- Related Disease
- Cancer
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunofluorescence: 1/50-1/200
Target
- Alternative Names
- NK2 Homeobox 1; Thyroid Transcription Factor 1; Thyroid-Specific Enhancer-Binding Protein; Homeobox Protein NK-2 Homolog A; Thyroid Nuclear Factor 1; NKX2A; TITF1; T/EBP; TTF-1; TTF1
- Gene ID
- 7080
- UniProt ID
- P43699
- Sequence Similarities
- Belongs to the NK-2 homeobox family.
- Cellular Localization
- Nucleus
- Post Translation Modifications
- Phosphorylated on serine residues by STK3/MST2. (P43699-NKX21_HUMAN)
Modification sites at PhosphoSitePlus (P43699)
Modification sites at neXtProt (NX_P43699)
Glycosylation from GlyGen (P43699) 1 site, 1 O-linked glycan (1 site)
- Protein Refseq
- NP_001073136.1; NP_003308.1
- Function
- May play a role in lung development and surfactant homeostasis. Forms a regulatory loop with GRHL2 that coordinates lung epithelial cell morphogenesis and differentiation. Activates the transcription of GNRHR and plays a role in enhancing the circadian oscillation of its gene expression.
Recommended Products
Isotype Control
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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IgG Antibody Products
Rabbit Monoclonal Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1310z | Mouse Anti-NKX2-1 Recombinant Antibody (clone 11B10) | IF, IHC, WB | Mouse IgG2b |
| ZG-0363U | Rabbit Anti-NKX2-1 Recombinant Antibody (clone A22-A) | ELISA, WB, IP | Rabbit IgG |
| ZG-0364U | Rabbit Anti-NKX2-1 Recombinant Antibody (clone G21-G) | IHC-P | Rabbit IgG |
| VS3-FY1045 | Recombinant Rabbit Anti-NKX2-1 Antibody (clone R02-6A5) | WB, IHC-P, IP | Rabbit IgG |
| VS13-YC811 | Rabbit Anti-NKX2-1 Recombinant Antibody (VS13-YC811) | WB, ICC, IF | Rabbit IgG |
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