Rabbit Anti-NKX2-1 Recombinant Antibody (clone R09-1H7)
CAT#: VS3-FY1918
This R09-1H7 recombinant monoclonal antibody is specifically designed to target human NKX2-1. It cross-reacts with hamster. It is suitable for use in a variety of assays, including WB, ICC/IF. The antibody has the positive detection of thyroid transcription factor 1 protein by western blot analysis of TSG101 in K562 lysates and immunocytochemistry analysis of thyroid transcription factor 1 (green) in K562 using thyroid transcription factor 1 antibody.
Tested Data
Gene Expression
WB
Figure 1 Anti-NKX2-1 Antibody (VS3-FY1918) in WB.
The TTF1 expression in K562 lysates were detected using the anti-TTF1 by western blot assay.
ICC
Figure 2 Anti-NKX2-1 Antibody (VS3-FY1918) in ICC.
Immunocytochemistry staining of Thyroid Transcription Factor 1 (green) in K562 using the anti-Thyroid Transcription Factor 1 antibody. Nuclear DNA was labeled with DAPI (shown in blue).
❮
❯
Subcellular Location
Figure 1 IF staining of human cell line HEK 293
Immunofluorescent staining of human cell line HEK 293 shows localization to the Golgi apparatus.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/74316/1569_C4_3_selected.jpg
Normal Tissue
Figure 2 Thyroid gland
Glandular cells
Staining:
High
Intensity: Strong
Quantity:>75%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/53633/117834_B_3_5.jpg
RNA Expression
Figure 3 RNA cell line category: Cell line enriched (SCLC-21H)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000136352-NKX2-1
❮
❯
Specifications
- Immunogen
- Synthetic peptide of human TTF1
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Hamster NKX2-1
- Species Reactivity
- Human, Hamster
- Clone
- R09-1H7
- Applications
- Western Blot, Immunofluorescence
- MW
- The calculated weight of the target protein is approximately 39 kDa, while the observed molecular weight stands at 39 kDa.
- Related Disease
- Cancer
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunofluorescence: 1/50-1/200
Target
- Alternative Names
- NK2 Homeobox 1; Thyroid Transcription Factor 1; Thyroid-Specific Enhancer-Binding Protein; Homeobox Protein NK-2 Homolog A; Thyroid Nuclear Factor 1; NKX2A; TITF1; T/EBP; TTF-1; TTF1
- Gene ID
- 7080
- UniProt ID
- P43699
- Sequence Similarities
- Belongs to the NK-2 homeobox family.
- Cellular Localization
- Nucleus
- Post Translation Modifications
- Phosphorylated on serine residues by STK3/MST2. (P43699-NKX21_HUMAN)
Modification sites at PhosphoSitePlus (P43699)
Modification sites at neXtProt (NX_P43699)
Glycosylation from GlyGen (P43699) 1 site, 1 O-linked glycan (1 site)
- Protein Refseq
- NP_001073136.1; NP_003308.1
- Function
- May play a role in lung development and surfactant homeostasis. Forms a regulatory loop with GRHL2 that coordinates lung epithelial cell morphogenesis and differentiation. Activates the transcription of GNRHR and plays a role in enhancing the circadian oscillation of its gene expression.
REVIEWS AND Q&AS
CITATIONS
RESOURCES
DOWNLOADS
RELATED PRODUCTS
Inquiry
Navs
Customer Review
There are currently no Customer reviews or questions for VS3-FY1918. Click the button above to contact us or submit your feedback about this product.
Distinct Nuclear Staining
We used clone R09-1H7 for immunofluorescence staining of K562 cells. It produced distinct nuclear staining, consistent with the localization of the transcription factor. The antibody is affinity purified and provided a strong signal at a 1:100 dilution, making it very efficient for our imaging.
Excellent Western Blot
This recombinant rabbit antibody worked perfectly in Western blot, detecting the target protein in cell lysates. We observed the expected band at approximately 39 kDa. Its ability to cross-react with hamster samples is also a beneficial feature for our specific animal model studies.
Q&As
-
What species does this NKX2-1 antibody cross-react with?
A: This recombinant monoclonal antibody (clone R09-1H7) is designed to target human NKX2-1 but also cross-reacts with Hamster. It binds to thyroid transcription factor 1 (TTF1).
-
What are the recommended dilutions for WB and IF?
A: For Western Blot, the recommended dilution is 1/500-1/1000. For Immunofluorescence, the recommended dilution is 1/50-1/200. The antibody has been validated using K562 lysates as a positive control.
View the frequently asked questions answered by Creative Biolabs Support.
Submit Your Publication
Published with our product? Submit your paper and receive a 10% discount on your next order! Share your research to earn exclusive rewards.
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "NKX2-1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1310z | Mouse Anti-NKX2-1 Recombinant Antibody (clone 11B10) | IF, IHC, WB | Mouse IgG2b |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-0342MZ | Recombinant Mouse Anti-Human NK2 Homeobox 1 Antibody (clone 9H8G4/2) | IHC | Mouse antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2449 | Hi-Affi™ Recombinant Rabbit Anti-NKX2-1 Monoclonal Antibody (DS2449AB) | IHC-P | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| ZG-0122C | Mouse Anti-NKX2-1 Recombinant Antibody (clone ABT-TTF1) | IHC-P, IF | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| ZG-0123C | Mouse Anti-NKX2-1 Recombinant Antibody (clone PT2256) | IHC-P, IF | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| ZG-0363U | Rabbit Anti-NKX2-1 Recombinant Antibody (clone A22-A) | ELISA, WB, IP | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY4850 | Anti-NKX2-1 Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY4851 | Anti-Mouse NKX2-1 Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY4853 | Anti-Human NKX2-1 Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY4852 | Anti-Rat NKX2-1 Immunohistochemistry Kit | IHC |
Specific Inquiry
Add to compare
See Our Custom Production in Action
Popular Products
Application: Neut, ELISA, IF, IP, FuncS, FC, ICC
Application: IP, IF, FuncS, FC, Neut, ELISA, ICC
Application: ELISA, FC, IP, FuncS, IF, Neut, ICC
Application: FC, IHC, FuncS, Inhib, Cyt
-2.png)
Application: ELISA, FC, IP, FuncS, IF, Neut, ICC
Application: ELISA, FC, IP, FuncS, IF, Neut, ICC
Application: ELISA, FC, IP, FuncS, IF, Neut, ICC
Application: FC, IP, ELISA, Neut, FuncS, IF, IHC
Application: WB, IHC, FC, Cyt, ELISA
Application: ELISA, IP, WB, IHC, IF, FuncS
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
Send Inquiry
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
