Rabbit Anti-NKX2-1 Recombinant Antibody (clone R09-1H7)
CAT#: VS3-FY1918
This R09-1H7 recombinant monoclonal antibody is specifically designed to target human NKX2-1. It cross-reacts with hamster. It is suitable for use in a variety of assays, including WB, ICC/IF. The antibody has the positive detection of thyroid transcription factor 1 protein by western blot analysis of TSG101 in K562 lysates and immunocytochemistry analysis of thyroid transcription factor 1 (green) in K562 using thyroid transcription factor 1 antibody.
Gene Expression
Tested Data
Subcellular Location
Figure 1 IF staining of human cell line HEK 293
Immunofluorescent staining of human cell line HEK 293 shows localization to the Golgi apparatus.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/74316/1569_C4_3_selected.jpg
Normal Tissue
Figure 2 Thyroid gland
Glandular cells
Staining:
High
Intensity: Strong
Quantity:>75%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/53633/117834_B_3_5.jpg
RNA Expression
Figure 3 RNA cell line category: Cell line enriched (SCLC-21H)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000136352-NKX2-1
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WB
Figure 1 Anti-NKX2-1 Antibody (VS3-FY1918) in WB.
The TTF1 expression in K562 lysates were detected using the anti-TTF1 by western blot assay.
ICC
Figure 2 Anti-NKX2-1 Antibody (VS3-FY1918) in ICC.
Immunocytochemistry staining of Thyroid Transcription Factor 1 (green) in K562 using the anti-Thyroid Transcription Factor 1 antibody. Nuclear DNA was labeled with DAPI (shown in blue).
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Specifications
- Immunogen
- Synthetic peptide of human TTF1
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Hamster NKX2-1
- Species Reactivity
- Human, Hamster
- Clone
- R09-1H7
- Applications
- Western Blot, Immunofluorescence
- MW
- The calculated weight of the target protein is approximately 39 kDa, while the observed molecular weight stands at 39 kDa.
- Related Disease
- Cancer
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunofluorescence: 1/50-1/200
Target
- Alternative Names
- NK2 Homeobox 1; Thyroid Transcription Factor 1; Thyroid-Specific Enhancer-Binding Protein; Homeobox Protein NK-2 Homolog A; Thyroid Nuclear Factor 1; NKX2A; TITF1; T/EBP; TTF-1; TTF1
- Gene ID
- 7080
- UniProt ID
- P43699
- Sequence Similarities
- Belongs to the NK-2 homeobox family.
- Cellular Localization
- Nucleus
- Post Translation Modifications
- Phosphorylated on serine residues by STK3/MST2. (P43699-NKX21_HUMAN)
Modification sites at PhosphoSitePlus (P43699)
Modification sites at neXtProt (NX_P43699)
Glycosylation from GlyGen (P43699) 1 site, 1 O-linked glycan (1 site)
- Protein Refseq
- NP_001073136.1; NP_003308.1
- Function
- May play a role in lung development and surfactant homeostasis. Forms a regulatory loop with GRHL2 that coordinates lung epithelial cell morphogenesis and differentiation. Activates the transcription of GNRHR and plays a role in enhancing the circadian oscillation of its gene expression.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "NKX2-1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2449 | Hi-Affi™ Recombinant Rabbit Anti-NKX2-1 Monoclonal Antibody (DS2449AB) | IHC-P | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| ZG-0122C | Mouse Anti-NKX2-1 Recombinant Antibody (clone ABT-TTF1) | IHC-P, IF | Mouse IgG |
| ZG-0123C | Mouse Anti-NKX2-1 Recombinant Antibody (clone PT2256) | IHC-P, IF | Mouse IgG |
| ZG-0487U | Rabbit Anti-NKX2-1 Recombinant Antibody (clone 6C11) | ELISA, WB, IHC, FC | Rabbit IgG |
| VS3-FY1045 | Recombinant Rabbit Anti-NKX2-1 Antibody (clone R02-6A5) | WB, IHC-P, IP | Rabbit IgG |
| VS13-YC811 | Rabbit Anti-NKX2-1 Recombinant Antibody (VS13-YC811) | WB, ICC, IF | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY4850 | Anti-NKX2-1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY4851 | Anti-Mouse NKX2-1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY4853 | Anti-Human NKX2-1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY4852 | Anti-Rat NKX2-1 Immunohistochemistry Kit | IHC |
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