Mouse Anti-NKX2-5 Monoclonal Antibody (clone 2E1)
CAT#: VS7-0425-WR483
This product is a mouse IgG1 antibody that binds to human NKX2-5. The antibody 2E1 can be used for WB and ELISA assays.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & cytosol.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/65034/1245_H8_3_selected.jpg
RNA Expression
Figure 2 RNA cell line category: Cell line enhanced (HEK93, NTERA-2, SK-MEL-30, U-2 OS, U-251 MG)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000183072-NKX2-5
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Specifications
- Immunogen
- Recombinant human NKX2-5 fragment expressed in E. Coli.
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Human NKX2-5
- Species Reactivity
- Human
- Clone
- 2E1
- Molecule Class
- Transcription Factor
- Applications
- WB, ELISA
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purity
- >95% as determined by SDS-PAGE and SEC-HPLC analysis, Ascitic fluid
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Preservative
- 0.03% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- Western Blot: 1:500 - 1:2000
Enzyme-linked Immunosorbent Assay: 1:10000
Target
- Alternative Names
- NKX2-5; NKX4-1; Homeobox Protein CSX; Cardiac-Specific Homeo Box; NKX2.5; NKX2E; NK2 Transcription Factor Related, Locus 5; CSX1; CSX; VSD3; Cardiac-Specific Homeobox; Homeobox Protein Nkx-2.5; Tinman Paralog
- Gene ID
- 1482
- UniProt ID
- P52952
- Long Name
- NK2 Homeobox 5
- Cellular Localization
- Nucleus
- Protein Refseq
- NP_001159647.1
- Function
- Developmental protein; DNA-binding
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "Clone 2E1"
- CAT
- Product Name
See other products for "NKX2-5"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-4074z | Mouse Anti-NKX2-5 Recombinant Antibody (clone 20D3) | WB, IP, IF, ELISA | Mouse IgG1, κ |
| MOB-0201MC | Recombinant Anti-Mouse NKX2-5 Antibody | ELISA, WB | |
| MOB-1726MZ | Recombinant Mouse Anti-Human NKX2-5 Antibody | WB | Mouse antibody |
| ZG-0315C | Mouse Anti-NKX2-5 Recombinant Antibody (ZG-0315C) | WB, ELISA | Mouse IgG |
| VS3-XY1167 | Mouse Anti-NKX2-5 Recombinant Antibody (clone 2E1) | ELISA, WB | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-4510 | Hi-Affi™ Recombinant Rabbit Anti-NKX2-5 Monoclonal Antibody (TH18DS) | ICC | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY4857 | Anti-NKX2-5 Immunohistochemistry Kit | IHC |
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