CytoStream™ Rabbit Anti-NLRP3 Recombinant Antibody (VS13-YC817)
CAT#: VS13-YC817
The CytoStream™ Rabbit Anti-NLRP3 Recombinant Antibody is specific for human, mouse and rat NLRP3. NLRP3 encodes a pyrin-like protein part of the NALP3 inflammasome, involved in innate immunity and inflammatory responses. Mutations relate to conditions like FCAS and MWS, with splicing variants diversifying its function. The anti-NLRP3 antibody was developed and selected by our CytoStream™ platform and the mAb is recommended for use in flow cytometry staining (1/50-1/100).
Gene Expression
Normal Tissue
Figure 1 IHC staining of human tonsil
Immunohistochemical staining of human tonsil shows moderate cytoplasmic positivity in germinal and non-germinal center cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/12878/29164_A_6_8_selected.jpg
Normal Tissue
Figure 2 Cerebral cortex
Endothelial cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous Glial cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous Neuronal cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/12878/29164_B_8_5.jpg
Normal Tissue
Figure 3 Colon
Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/12878/29164_A_8_3.jpg
Normal Tissue
Figure 4 Testis
Cells in seminiferous ducts Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous Leydig cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/12878/29164_A_6_6.jpg
Normal Tissue
Figure 5 Bone marrow
Hematopoietic cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/12878/29164_B_5_4.jpg
Normal Tissue
Figure 6 Lymph node
Germinal center cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/12878/29164_A_8_8.jpg
RNA Expression
Figure 7 RNA cell line category: Cell line enriched (THP-1)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000162711-NLRP3
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Specifications
- Immunogen
- Recombinant human NLRP3, aa 5-161.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat NLRP3
- Species Reactivity
- Human, Mouse, Rat
- Applications
- WB, IHC-P, ICC, IF, FC
- Conjugate
- NONE
- Related Disease
- Familial cold autoinflammatory syndrome (FCAS), Muckle-Wells syndrome (MWS), Chronic infantile neurological cutaneous and articular (CINCA) syndrome, and Neonatal-onset multisystem inflammatory disease (NOMID)
Product Property
- Clonality
- Monoclonal
- Purification
- Protein A purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- TBS (pH 7.4) containing 40% Glycerol and 0.05% BSA.
- Preservative
- 0.05% Sodium azide
- Storage
- Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Shipping with ice pack.
Applications
- Application Notes
- Western blot (WB): 1/500-1/1000
Immunocytochemistry (ICC): 1/100-1/500
Immunofluorescence (IF): 1/100-1/500
Immunohistochemistry-paraffin (IHC-P): 1/50-1/400
Flow cytometry (FC): 1/50-1/100
Target
- Alternative Names
- NLR Family Pyrin Domain Containing 3; Cryopyrin; Nucleotide-Binding Oligomerization Domain, Leucine Rich Repeat And Pyrin Domain Containing 3; Cold-Induced Autoinflammatory Syndrome 1 Protein; PYRIN-Containing APAF1-Like Protein 1; Caterpiller Protein 1.1; C1orf7; CLR1.1; PYPAF1; CIAS1; NALP3; NACHT Domain-, Leucine-Rich Repeat-, And PYD-Containing Protein 3; NACHT, LRR And PYD Domains-Containing Protein 3;
- Gene ID
- 114548
- UniProt ID
- Q96P20
- Long Name
- NLR Family Pyrin Domain Containing 3
- Cellular Localization
- Secreted; Nucleus; Endoplasmic reticulum; Cytoplasm; Inflammasome
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "NLRP3"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2811z | Mouse Anti-NLRP3 Recombinant Antibody (MOB-2811z) | WB, IHC, IHC-Fr, IHC-P, IP | Mouse IgG1 |
| MOB-2139MZ | Recombinant Mouse Anti-Human NLRP3 Antibody (clone Obmqz4-b) | ICC, WB | Mouse antibody |
| MRO-1097-CN | Recombinant Rabbit Anti-NLRP3 Monoclonal Antibody (CBACN-403) | WB, IF, IHC, IP, FC | Rabbit IgG |
| VS3-WK1107 | Rabbit Anti-NLRP3 Recombinant Antibody (VS3-WK1107) | WB, ICC, IF, IHC, IP, FC | Rabbit IgG |
| VS13-YC816 | Rabbit Anti-NLRP3 Polyclonal Antibody (VS13-YC816) | WB, IHC, IF | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2811z-F(E) | Recombinant Anti-Human NLRP3 Antibody Fab Fragment | WB, FuncS | Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2811z-S(P) | Recombinant Anti-Human NLRP3 Antibody scFv Fragment | ELISA, IF, FuncS | scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2453 | Hi-Affi™ Recombinant Rabbit Anti-NLRP3 Monoclonal Antibody (DS2453AB) | FC, IP, WB | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1499 | Anti-NLRP3 Immunohistochemistry Kit | IHC | |
| VS-0525-XY4870 | Anti-Mouse NLRP3 Immunohistochemistry Kit | IHC | |
| VS-0525-XY4869 | Anti-Human NLRP3 Immunohistochemistry Kit | IHC |
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