Rabbit Anti-NME1 Recombinant Antibody (clone R01-6C0) (CAT#: VS3-FY2049)
The anti-NM23A antibody can be tested in western blot analysis of NM23A in Hela, A549, MCF-7, 293T lysates. The recombinant monoclonal antibody demonstrates binding to human NME1. The R01-6C0 is recommended for dilution of target protein by western blot (dilution 1/500-1/1000).
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WB
Figure 1 Anti-NME1 Antibody (VS3-FY2049) in WB.
The NM23A expression in Hela, A549, MCF-7, 293T lysates were detected using the anti-NM23A by western blot assay.
Specifications
- Immunogen
- Synthetic peptide of human NM23A
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human NME1
- Species Reactivity
- Human
- Clone
- R01-6C0
- Applications
- Western Blot
- MW
- The calculated weight of the target protein is approximately 17 kDa, while the observed molecular weight stands at 17 kDa.
- Related Disease
- Neuroblastoma 1 and Lung Large Cell Carcinoma
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Target
- Alternative Names
- NME/NM23 Nucleoside Diphosphate Kinase 1; Non-Metastatic Cells 1, Protein (NM23A) Expressed In; Tumor Metastatic Process-Associated Protein; Metastasis Inhibition Factor Nm23; Granzyme A-Activated DNase; NDP Kinase A; EC 2.7.4.6; NM23-H1; NDPKA; GAAD
- Gene ID
- 4830
- UniProt ID
- P15531
- Sequence Similarities
- Belongs to the NDK family.
- Cellular Localization
- Cytoplasm, Nucleus
- Post Translation Modifications
- Ubiquitination at Lys100 (NX_P15531 [NX_P15531-1])
Modification sites at PhosphoSitePlus (P15531)
Glycosylation from GlyGen (P15531) 1 site, 1 O-linked glycan (1 site)
- Protein Refseq
- NP_000260.1; NP_937818.1
- Function
- Major role in the synthesis of nucleoside triphosphates other than ATP. The ATP gamma phosphate is transferred to the NDP beta phosphate via a ping-pong mechanism, using a phosphorylated active-site intermediate. Possesses nucleoside-diphosphate kinase, serine/threonine-specific protein kinase, geranyl and farnesyl pyrophosphate kinase, histidine protein kinase and 3'-5' exonuclease activities.
Recommended Products
Isotype Control
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0394MZ | Chicken Anti-NME1 Polyclonal IgY | WB | Chicken antibody |
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