Rabbit Anti-NME1 Recombinant Antibody (clone R01-6C0)
CAT#: VS3-FY2049
The anti-NM23A antibody can be tested in western blot analysis of NM23A in Hela, A549, MCF-7, 293T lysates. The recombinant monoclonal antibody demonstrates binding to human NME1. The R01-6C0 is recommended for dilution of target protein by western blot (dilution 1/500-1/1000).
Gene Expression
Tested Data
Subcellular Location
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/8467/if_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm, plasma membrane & cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/40571/if_selected.jpg
Normal Tissue
Figure 3 Lymph node
Germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/40571/84790_A_7_8.jpg
Normal Tissue
Figure 4 Colon
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/40571/84790_A_8_3.jpg
Normal Tissue
Figure 5 Kidney
Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/40571/84790_A_9_5.jpg
Normal Tissue
Figure 6 Testis
Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Sertoli cells Staining: Low Intensity: Moderate Quantity: <25% Spermatogonia cells Staining: Medium Intensity: Moderate
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/40571/84790_A_4_6.jpg
RNA Expression
Figure 7 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000239672-NME1
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WB
Figure 1 Anti-NME1 Antibody (VS3-FY2049) in WB.
The NM23A expression in Hela, A549, MCF-7, 293T lysates were detected using the anti-NM23A by western blot assay.
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Specifications
- Immunogen
- Synthetic peptide of human NM23A
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human NME1
- Species Reactivity
- Human
- Clone
- R01-6C0
- Applications
- Western Blot
- MW
- The calculated weight of the target protein is approximately 17 kDa, while the observed molecular weight stands at 17 kDa.
- Related Disease
- Neuroblastoma 1 and Lung Large Cell Carcinoma
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Target
- Alternative Names
- NME/NM23 Nucleoside Diphosphate Kinase 1; Non-Metastatic Cells 1, Protein (NM23A) Expressed In; Tumor Metastatic Process-Associated Protein; Metastasis Inhibition Factor Nm23; Granzyme A-Activated DNase; NDP Kinase A; EC 2.7.4.6; NM23-H1; NDPKA; GAAD
- Gene ID
- 4830
- UniProt ID
- P15531
- Sequence Similarities
- Belongs to the NDK family.
- Cellular Localization
- Cytoplasm, Nucleus
- Post Translation Modifications
- Ubiquitination at Lys100 (NX_P15531 [NX_P15531-1])
Modification sites at PhosphoSitePlus (P15531)
Glycosylation from GlyGen (P15531) 1 site, 1 O-linked glycan (1 site)
- Protein Refseq
- NP_000260.1; NP_937818.1
- Function
- Major role in the synthesis of nucleoside triphosphates other than ATP. The ATP gamma phosphate is transferred to the NDP beta phosphate via a ping-pong mechanism, using a phosphorylated active-site intermediate. Possesses nucleoside-diphosphate kinase, serine/threonine-specific protein kinase, geranyl and farnesyl pyrophosphate kinase, histidine protein kinase and 3'-5' exonuclease activities.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
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| BRD-0394MZ | Chicken Anti-NME1 Polyclonal IgY | WB | Chicken antibody |
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| CAT | Product Name | Application | Type |
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| MRO-2077-CN | Rabbit Anti-NME1 Polyclonal Antibody (MRO-2077-CN) | IF, IHC, FC, WB | Rabbit IgG |
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| CAT | Product Name | Application | Type |
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| VS-0724-YC686 | AbPlus™ Anti-NME1 Magnetic Beads (VS-0724-YC686) | IP, Protein Purification | |
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1500 | Anti-NME1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY4874 | Anti-Mouse NME1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY4873 | Anti-Human NME1 Immunohistochemistry Kit | IHC |
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