Rabbit Anti-NME1 Recombinant Antibody (VS13-YC818)
CAT#: VS13-YC818
The Rabbit Anti-NME1 Recombinant Antibody is specific for human NME1. NME1 encodes nucleoside diphosphate kinase contributing to nucleoside triphosphate synthesis, affecting metastasis in tumors like neuroblastomas, with splice variants and NME2 fusion transcripts noted. The anti-NME1 antibody can be used for Western blot (1/500-1/1000).
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/8467/if_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm, plasma membrane & cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/40571/if_selected.jpg
Normal Tissue
Figure 3 Lymph node
Germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/40571/84790_A_7_8.jpg
Normal Tissue
Figure 4 Colon
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/40571/84790_A_8_3.jpg
Normal Tissue
Figure 5 Kidney
Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/40571/84790_A_9_5.jpg
Normal Tissue
Figure 6 Testis
Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Sertoli cells Staining: Low Intensity: Moderate Quantity: <25% Spermatogonia cells Staining: Medium Intensity: Moderate
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/40571/84790_A_4_6.jpg
RNA Expression
Figure 7 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000239672-NME1
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Specifications
- Immunogen
- Recombinant human NM23A.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human NME1
- Species Reactivity
- Human
- Applications
- WB
- Conjugate
- NONE
- Related Disease
- Neuroblastoma and Lung Large Cell Carcinoma
Product Property
- Clonality
- Monoclonal
- Purification
- Affinity Chromatography purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- 50 mM Tris-Glycine (pH 7.4) containing 150 mM NaCl, 40% Glycerol and 0.05% BSA.
- Preservative
- 0.01% Sodium azide
- Storage
- Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Shipping with ice pack.
Applications
- Application Notes
- Western blot (WB): 1/500-1/1000
Target
- Alternative Names
- NME/NM23 Nucleoside Diphosphate Kinase 1; Non-Metastatic Cells 1, Protein (NM23A) Expressed In; Tumor Metastatic Process-Associated Protein; Metastasis Inhibition Factor Nm23; Granzyme A-Activated DNase; NDP Kinase A; EC 2.7.4.6; NM23-H1; NDPKA; GAAD;
- Gene ID
- 4830
- UniProt ID
- P15531
- Long Name
- NME/NM23 Nucleoside Diphosphate Kinase 1
- Sequence Similarities
- Belongs to the NDK family.
- Cellular Localization
- Cytoplasm; Nucleus
- Protein Refseq
- NP_000260.1; NP_937818.1
- Function
- Major role in the synthesis of nucleoside triphosphates other than ATP. The ATP gamma phosphate is transferred to the NDP beta phosphate via a ping-pong mechanism, using a phosphorylated active-site intermediate. Possesses nucleoside-diphosphate kinase, serine/threonine-specific protein kinase, geranyl and farnesyl pyrophosphate kinase, histidine protein kinase and 3'-5' exonuclease activities.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "NME1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0394MZ | Chicken Anti-NME1 Polyclonal IgY | WB | Chicken antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2456 | Hi-Affi™ Recombinant Rabbit Anti-NME1 Monoclonal Antibody (DS2456AB) | ICC, IF, IHC-P, WB | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-2077-CN | Rabbit Anti-NME1 Polyclonal Antibody (MRO-2077-CN) | IF, IHC, FC, WB | Rabbit IgG |
| MRO-2078-CN | Rabbit Anti-NME1 Polyclonal Antibody (MRO-2078-CN) | WB, IF, IHC, FC | Rabbit IgG |
| VS3-QX821 | Mouse Anti-NME1 Recombinant Antibody (clone 1H9-F3-G5) | WB | Mouse IgG1 |
| VS3-QX822 | Mouse Anti-NME1 Recombinant Antibody (clone 1172CT2.4.1.1) | WB, IHC | Mouse IgG2a |
| VS3-QX823 | Mouse Anti-NME1 Recombinant Antibody (clone 12A1) | WB, ICC, IHC | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC686 | AbPlus™ Anti-NME1 Magnetic Beads (VS-0724-YC686) | IP, Protein Purification | |
| VS-0724-YC899 | AbPlus™ Anti-NME1 Magnetic Beads (VS-0724-YC899) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1500 | Anti-NME1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY4874 | Anti-Mouse NME1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY4873 | Anti-Human NME1 Immunohistochemistry Kit | IHC |
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