Mouse Anti-NME1 Recombinant Antibody (VS3-CJ675)
CAT#: VS3-CJ675
This product is a mouse antibody that recognizes human, and rat NME1.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/8467/if_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm, plasma membrane & cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/40571/if_selected.jpg
Normal Tissue
Figure 3 Lymph node
Germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/40571/84790_A_7_8.jpg
Normal Tissue
Figure 4 Colon
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/40571/84790_A_8_3.jpg
Normal Tissue
Figure 5 Kidney
Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/40571/84790_A_9_5.jpg
Normal Tissue
Figure 6 Testis
Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Sertoli cells Staining: Low Intensity: Moderate Quantity: <25% Spermatogonia cells Staining: Medium Intensity: Moderate
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/40571/84790_A_4_6.jpg
RNA Expression
Figure 7 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000239672-NME1
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Specifications
- Immunogen
- Purified His-tagged NME1 protein
- Host Species
- Mouse
- Type
- Mouse IgG2a
- Specificity
- Human, Rat NME1
- Species Reactivity
- Human, Rat
- Applications
- WB, IHC-P
- Conjugate
- Unconjugated
Product Property
- Purification
- Protein G affinity purified
- Purity
- >95% as determined by SDS-PAGE
- Format
- Liquid
- Buffer
- PBS, pH 7.3.
- Preservative
- 0.09% Sodium Azide
- Storage
- Store at 4°C for short term. Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Western Blot (1:1000), Immunohistochemistry-Parraffin (1:25).
Target
- Alternative Names
- NB; AWD; NBS; GAAD; NDKA; NM23; NDPKA; NDPK-A; NM23-H1
- Gene ID
- 4830
- UniProt ID
- P15531
- Sequence Similarities
- Belongs to the NDK family.
- Cellular Localization
- Cytoplasm, Nucleus
- Protein Refseq
- NP_000260.1; NP_937818.1
- Function
- Major role in the synthesis of nucleoside triphosphates other than ATP. The ATP gamma phosphate is transferred to the NDP beta phosphate via a ping-pong mechanism, using a phosphorylated active-site intermediate. Possesses nucleoside-diphosphate kinase, serine/threonine-specific protein kinase, geranyl and farnesyl pyrophosphate kinase, histidine protein kinase and 3'-5' exonuclease activities. Involved in cell proliferation, differentiation and development, signal transduction, G protein- coupled receptor endocytosis, and gene expression. Required for neural development including neural patterning and cell fate determination. During GZMA-mediated cell death, works in concert with TREX1. NME1 nicks one strand of DNA and TREX1 removes bases from the free 3' end to enhance DNA damage and prevent DNA end reannealing and rapid repair.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Secondary Antibody
- CAT
- Product Name
Recommended Dilution Buffer
- CAT
- Product Name
See other products for "NME1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2816z | Mouse Anti-NME1 Recombinant Antibody (clone 33H11) | WB, ELISA, FC, ICC, IF, IHC | Mouse IgG |
| MOB-1729MZ | Recombinant Mouse Anti-Human NME1 Antibody (clone 48.7) | IHC-Fr, IHC-P, WB | Mouse antibody |
| MRO-2077-CN | Rabbit Anti-NME1 Polyclonal Antibody (MRO-2077-CN) | IF, IHC, FC, WB | Rabbit IgG |
| MRO-2078-CN | Rabbit Anti-NME1 Polyclonal Antibody (MRO-2078-CN) | WB, IF, IHC, FC | Rabbit IgG |
| VS3-QX821 | Mouse Anti-NME1 Recombinant Antibody (clone 1H9-F3-G5) | WB | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0394MZ | Chicken Anti-NME1 Polyclonal IgY | WB | Chicken antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2456 | Hi-Affi™ Recombinant Rabbit Anti-NME1 Monoclonal Antibody (DS2456AB) | ICC, IF, IHC-P, WB | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC686 | AbPlus™ Anti-NME1 Magnetic Beads (VS-0724-YC686) | IP, Protein Purification | |
| VS-0724-YC899 | AbPlus™ Anti-NME1 Magnetic Beads (VS-0724-YC899) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1500 | Anti-NME1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY4874 | Anti-Mouse NME1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY4873 | Anti-Human NME1 Immunohistochemistry Kit | IHC |
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