Rabbit Anti-NOLC1 Recombinant Antibody (VS13-YC823) (CAT#: VS13-YC823)
The Rabbit Anti-NOLC1 Recombinant Antibody is specific for human NOLC1. NOLC1 is a nucleolar protein regulating RNA polymerase I, crucial for ribosomal processing and neural crest specification through interactions with ribosomal modification enzymes. The anti-NOLC1 antibody can be used for Western blot (1/500-1/1000), Immunohistochemistry (1/50-1/100), Immunofluorescence (1/50-1/200).
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Subcellular Location and Protein Expression
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoli fibrillar center.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/37366/453_F3_1_selected.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoli fibrillar center.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/37366/453_F3_1_selected.jpg
Figure 2 IF staining of human cell line HEK 293
(Immunofluorescent staining of human cell line HEK 293 shows localization to nucleoli fibrillar center.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/50388/if_selected.jpg
(Immunofluorescent staining of human cell line HEK 293 shows localization to nucleoli fibrillar center.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/50388/if_selected.jpg
Figure 3 IHC staining of human cerebral cortex
(Immunohistochemical staining of human cerebral cortex shows moderate nucleolar positivity in neuronal cells.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/37366/ihc_selected.jpg
(Immunohistochemical staining of human cerebral cortex shows moderate nucleolar positivity in neuronal cells.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/37366/ihc_selected.jpg
Figure 4 IHC staining of human esophagus
(Immunohistochemical staining of human esophagus shows moderate nucleolar positivity in squamous epithelial cells.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/32654/ihc_selected.jpg
(Immunohistochemical staining of human esophagus shows moderate nucleolar positivity in squamous epithelial cells.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/32654/ihc_selected.jpg
Figure 5 IF staining of human cell line A-431
(Immunofluorescent staining of human cell line A-431 shows localization to nucleoli fibrillar center.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/37366/434_F3_3_blue_red_green.jpg
(Immunofluorescent staining of human cell line A-431 shows localization to nucleoli fibrillar center.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/37366/434_F3_3_blue_red_green.jpg
Figure 6 IF staining of human cell line U-251 MG
(Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoli fibrillar center.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/37366/450_F3_1_blue_red_green.jpg
(Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoli fibrillar center.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/37366/450_F3_1_blue_red_green.jpg
Figure 7 Cerebral cortex
(Neuronal cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/37366/150360_B_7_5.jpg
(Neuronal cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/37366/150360_B_7_5.jpg
Figure 8 Testis
(Leydig cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/37366/150360_A_6_6.jpg
(Leydig cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/37366/150360_A_6_6.jpg
Figure 9 Tonsil
(Squamous epithelial cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/37366/150360_A_4_8.jpg
(Squamous epithelial cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/37366/150360_A_4_8.jpg
Figure 10 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000166197-NOLC1
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000166197-NOLC1
Specifications
- Immunogen
- Recombinant human NOLC1.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human NOLC1
- Species Reactivity
- Human
- Applications
- WB, IHC-Fr, IHC-P, ICC, IF
- Conjugate
- NONE
Product Property
- Clonality
- Monoclonal
- Purification
- Affinity Chromatography purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- 50 mM Tris-Glycine (pH 7.4) containing 150 mM NaCl, 40% Glycerol and 0.05% BSA.
- Preservative
- 0.01% Sodium azide
- Storage
- Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Shipping with ice pack.
Applications
- Application Notes
- Western blot (WB): 1/500-1/1000
Immunohistochemistry (IHC): 1/50-1/100
Immunofluorescence (IF): 1/50-1/200
Target
- Alternative Names
- Nucleolar And Coiled-Body Phosphoprotein 1; Hepatitis C Virus NS5A-Transactivated Protein 13; HCV NS5A-Transactivated Protein 13; 140 KDa Nucleolar Phosphoprotein; Nucleolar Phosphoprotein P130; Nucleolar 130 KDa Protein; NS5ATP13; NOPP140;
- Gene ID
- 9221
- UniProt ID
- Q14978
- Long Name
- Nucleolar And Coiled-Body Phosphoprotein 1
Recommended Products
Secondary Antibody
Isotype Control
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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IgG Antibody Products
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IHC Kit and Antibody Products: Precision Tools for Immunohistochemistry
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-3019z | Mouse Anti-NOLC1 Recombinant Antibody (clone 35H12) | WB, IF, IHC, IP | Mouse IgG1 |
| MOB-0794CT | Recombinant Mouse anti-Human NOLC1 Monoclonal antibody (EML919) | WB |
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