Rabbit Anti-NPHS1 Recombinant Antibody (clone D13-E), FITC
CAT#: ZG-0443U
Rabbit anti-NPHS1 antibody (clone D13-E) was conjugated with FITC under optimal conditions. The FITC-conjugated antibody product displayed high specificity against the NPHS1 protein of Human. And the solution is free of unconjugated FITC and unconjugated antibody.
Tested Data
Gene Expression
FC
Figure 1 Rabbit Anti-NPHS1 Antibody (ZG-0443U) in FC
Profile of nephrin expession in human Hek293 cell line analyzed by the BD FACSCanto II. Cells were fixed, permeabilized and stained with anti-human Nephrin FITC (green, used 10 μl per test) or with an isotype control (black).
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Normal Tissue
Figure 1 Cerebral cortex
Endothelial cells Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous Neuronal cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: Nuclear Neuropil Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/35555/67641_B_8_5.jpg
Normal Tissue
Figure 2 Colon
Endothelial cells Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/35555/67641_A_9_3.jpg
Normal Tissue
Figure 3 High expression in kidney.
Cells in glomeruli Staining: High Intensity: Strong Quantity: 75%-25% Distal tubules Staining: Low Intensity: Weak Quantity:>75%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/35555/67641_A_9_5.jpg
Normal Tissue
Figure 4 Testis
Cells in seminiferous ducts Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous Leydig cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/35555/67641_A_4_6.jpg
Normal Tissue
Figure 5 Lymph node
Germinal center cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/35555/67641_A_9_8.jpg
RNA Expression
Figure 6 RNA cell line category: Cell line enhanced (HMC-1, JURKAT)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000161270-NPHS1
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Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human NPHS1
- Species Reactivity
- Human
- Clone
- D13-E
- Applications
- FC
- Conjugate
- FITC
- Related Disease
- Nephrotic Syndrome, Type 1 and Congenital Nephrotic Syndrome Finnish Type.
Product Property
- Buffer
- Aqueous buffer solution containing 10mg/ml of BSA and 0.05% sodium azide.
- Preservative
- 0.05% sodium azide
- Storage
- Store at 4°C, in the dark. Do not freeze.
Applications
- Application Notes
- This antibody has been used 10 µL per test for Flow Cytometry. Specific conditions should be optimized by the end user.
Target
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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