Recombinant Anti-Human NPHS1 Antibody Fab Fragment
CAT#: MOB-1833z-F(E)
Recombinant Mouse Antibody Fab Fragment is against Human NPHS1, expressed in Chinese Hamster Ovary cells(CHO).
Gene Expression
Normal Tissue
Figure 1 Cerebral cortex
Endothelial cells Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous Neuronal cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: Nuclear Neuropil Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/35555/67641_B_8_5.jpg
Normal Tissue
Figure 2 Colon
Endothelial cells Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/35555/67641_A_9_3.jpg
Normal Tissue
Figure 3 High expression in kidney.
Cells in glomeruli Staining: High Intensity: Strong Quantity: 75%-25% Distal tubules Staining: Low Intensity: Weak Quantity:>75%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/35555/67641_A_9_5.jpg
Normal Tissue
Figure 4 Testis
Cells in seminiferous ducts Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous Leydig cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/35555/67641_A_4_6.jpg
Normal Tissue
Figure 5 Lymph node
Germinal center cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/35555/67641_A_9_8.jpg
RNA Expression
Figure 6 RNA cell line category: Cell line enhanced (HMC-1, JURKAT)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000161270-NPHS1
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Specifications
- Immunogen
- Human Nephrosis 1, Congenital, Finnish Type (Nephrin)
- Host Species
- Mouse
- Derivation
- Mouse
- Type
- Fab
- Specificity
- Tested positive against native Human Nephrosis 1, Congenital, Finnish Type (Nephrin)
- Species Reactivity
- Human
- Applications
- Can be useful in applications such as: Immunofluorescence; Neutralization; Functional Study
Product Property
- Purity
- >95% as determined by analysis by SDS-PAGE
- Storage
- 4°C or -20°C, avoid repeated freezing and thawing.
Target
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Focused Kidney Research Utility
The Anti-Human NPHS1 Fab Fragment has been a key tool in our kidney research. Its ability to target nephrin specifically helps us shed light on its role in podocyte function and integrity, which is crucial for our studies into nephrotic syndrome mechanisms.
Exemplary for Glomerular Studies
This Fab fragment has been exemplary in our glomerular research, allowing us to zero in on nephrin's role in maintaining filtration barrier function. The crisp results in our immunofluorescence assays have highlighted differences between healthy and diseased states, aiding our understanding of glomerular pathologies.
Superior Tissue Penetration
The Fab fragment's small size allows it to penetrate tissues more effectively than full-sized antibodies, giving us enhanced resolution in heavily structured areas within our kidney tissue studies.
Q&As
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What is the main target of the NPHS1 Antibody Fab Fragment?
A: This Fab fragment targets the NPHS1 protein, also known as nephrin, which is essential for kidney function and is a critical component of the slit diaphragm in podocytes. It plays a significant role in research focused on understanding kidney diseases and renal physiology.
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What are the specific applications for this Fab fragment?
A: The Fab fragment is used in applications such as Western blotting (WB), immunohistochemistry (IHC), and protein localization studies. It helps researchers investigate nephrin's role in filtration barrier integrity and related cellular mechanisms.
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How does this Fab fragment benefit kidney disease research?
A: By specifically binding to NPHS1, this fragment allows detailed examination of nephrin expression and function in healthy and diseased tissues, providing insights crucial for understanding various kidney disorders, including nephrotic syndrome.
View the frequently asked questions answered by Creative Biolabs Support.
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Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
See other products for "NPHS1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1833z-S(P) | Recombinant Anti-Human NPHS1 Antibody scFv Fragment | WB, Neut, FuncS | scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2478 | Hi-Affi™ Recombinant Rabbit Anti-NPHS1 Monoclonal Antibody (DS2478AB) | WB, ELISA, IP, IHC-P | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-165CQ | Mouse Anti-NPHS1 Antibody | ELISA, WB | Mouse IgG1, κ |
| ZG-0340U | Rabbit Anti-NPHS1 Recombinant Antibody (clone Y17-R) | ELISA, WB, IP | Rabbit IgG |
| ZG-0344U | Rabbit Anti-NPHS1 Recombinant Antibody (clone G17-H) | ELISA, WB, IP | Rabbit IgG |
| ZG-0443U | Rabbit Anti-NPHS1 Recombinant Antibody (clone D13-E), FITC | FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY4918 | Anti-NPHS1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY4919 | Anti-Mouse NPHS1 Immunohistochemistry Kit | IHC |
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