Rabbit Anti-NRAS Recombinant Antibody (VS13-YC842)
CAT#: VS13-YC842
The Rabbit Anti-NRAS Recombinant Antibody is specific for human and rat NRAS. NRAS encodes a membrane GTPase regulated by palmitoylation, linked to multiple cancers and syndromes like Noonan and autoimmune lymphoproliferative disorders, showing splice variants. The anti-NRAS antibody can be used for Western blot (1/1000-1/2000), Immunocytochemistry (1/100-1/500), Immunofluorescence (1/100-1/500).
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/79912/1987_E10_1_selected.jpg
Normal Tissue
Figure 2 IHC staining of human pancreas
Immunohistochemical staining of human pancreas shows strong cytoplasmic positivity in exocrine glandular cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/10157/ihc_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/49830/118215_B_7_5.jpg
Normal Tissue
Figure 4 Colon
Endothelial cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/49830/118215_A_9_3.jpg
Normal Tissue
Figure 5 Lymph node
Germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear Non-germinal center cells Staining: Not detected Intensity: Weak Quantity: <25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/49830/118215_A_8_8.jpg
RNA Expression
Figure 6 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000213281-NRAS
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Specifications
- Immunogen
- Recombinant human Ras, aa 21-64.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human and Rat NRAS
- Species Reactivity
- Human, Rat
- Applications
- WB, ICC, IF, IP
- Conjugate
- NONE
Product Property
- Clonality
- Monoclonal
- Purification
- Protein A purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- TBS (pH 7.4) containing 40% Glycerol and 0.05% BSA.
- Preservative
- 0.05% Sodium azide
- Storage
- Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Shipping with ice pack.
Applications
- Application Notes
- Western blot (WB): 1/1000-1/2000
Immunocytochemistry (ICC): 1/100-1/500
Immunofluorescence (IF): 1/100-1/500
Target
- Alternative Names
- NRAS Proto-Oncogene, GTPase; Neuroblastoma RAS Viral (V-Ras) Oncogene Homolog; Neuroblastoma RAS Viral Oncogene Homolog; Transforming Protein N-Ras; V-Ras Neuroblastoma RAS Viral Oncogene Homolog; N-Ras Protein Part 4; GTPase NRas; HRAS1;
- Gene ID
- 4893
- UniProt ID
- P01111
- Long Name
- NRAS Proto-Oncogene, GTPase
- Cellular Localization
- Nucleus; Cytoplasm; Cell membrane; Golgi apparatus
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "NRAS"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1454CT | Recombinant Mouse anti-Human NRAS Monoclonal antibody (BU3H0) | ELISA, WB | |
| MRO-1321-CN | Recombinant Rabbit Anti-NRAS Monoclonal Antibody (CBACN-478) | WB, IP, FC | Rabbit IgG |
| MRO-1322-CN | Recombinant Rabbit Anti-NRAS Monoclonal Antibody (JF10-11) | WB, IF, IP | Rabbit IgG |
| VS3-CJ234 | Mouse Anti-NRAS Recombinant Antibody (VS3-CJ234) | WB | Mouse IgG1 |
| VS3-FY2077 | Rabbit Anti-NRAS Recombinant Antibody (clone R01-7D0) | WB, IP | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2498 | Hi-Affi™ Recombinant Rabbit Anti-NRAS Monoclonal Antibody (DS2498AB) | WB, IP | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHC-YF595 | A*01:01/Human N-RAS (ILDTAGREEY) MHC Pentamer | FCM |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0424-XY205 | AbPlus™ Anti-NRAS Magnetic Beads (JF10-11) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1518 | Anti-NRAS Immunohistochemistry Kit | IHC | |
| VS-0525-XY4973 | Anti-Mouse NRAS Immunohistochemistry Kit | IHC | |
| VS-0525-XY4972 | Anti-Human NRAS Immunohistochemistry Kit | IHC |
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