Rabbit Anti-NRAS Recombinant Antibody (VS13-YC842) (CAT#: VS13-YC842)
The Rabbit Anti-NRAS Recombinant Antibody is specific for human and rat NRAS. NRAS encodes a membrane GTPase regulated by palmitoylation, linked to multiple cancers and syndromes like Noonan and autoimmune lymphoproliferative disorders, showing splice variants. The anti-NRAS antibody can be used for Western blot (1/1000-1/2000), Immunocytochemistry (1/100-1/500), Immunofluorescence (1/100-1/500).
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We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.
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Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/79912/1987_E10_1_selected.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/79912/1987_E10_1_selected.jpg
Figure 2 IHC staining of human pancreas
(Immunohistochemical staining of human pancreas shows strong cytoplasmic positivity in exocrine glandular cells.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/10157/ihc_selected.jpg
(Immunohistochemical staining of human pancreas shows strong cytoplasmic positivity in exocrine glandular cells.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/10157/ihc_selected.jpg
Figure 3 Cerebral cortex
(Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/49830/118215_B_7_5.jpg
(Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/49830/118215_B_7_5.jpg
Figure 4 Colon
(Endothelial cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/49830/118215_A_9_3.jpg
(Endothelial cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/49830/118215_A_9_3.jpg
Figure 5 Lymph node
(Germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear Non-germinal center cells Staining: Not detected Intensity: Weak Quantity: <25% Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/49830/118215_A_8_8.jpg
(Germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear Non-germinal center cells Staining: Not detected Intensity: Weak Quantity: <25% Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/49830/118215_A_8_8.jpg
Figure 6 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000213281-NRAS
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000213281-NRAS
Specifications
- Immunogen
- Recombinant human Ras, aa 21-64.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human and Rat NRAS
- Species Reactivity
- Human, Rat
- Applications
- WB, ICC, IF, IP
- Conjugate
- NONE
Product Property
- Clonality
- Monoclonal
- Purification
- Protein A purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- TBS (pH 7.4) containing 40% Glycerol and 0.05% BSA.
- Preservative
- 0.05% Sodium azide
- Storage
- Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Shipping with ice pack.
Applications
- Application Notes
- Western blot (WB): 1/1000-1/2000
Immunocytochemistry (ICC): 1/100-1/500
Immunofluorescence (IF): 1/100-1/500
Target
- Alternative Names
- NRAS Proto-Oncogene, GTPase; Neuroblastoma RAS Viral (V-Ras) Oncogene Homolog; Neuroblastoma RAS Viral Oncogene Homolog; Transforming Protein N-Ras; V-Ras Neuroblastoma RAS Viral Oncogene Homolog; N-Ras Protein Part 4; GTPase NRas; HRAS1;
- Gene ID
- 4893
- UniProt ID
- P01111
- Long Name
- NRAS Proto-Oncogene, GTPase
- Cellular Localization
- Nucleus; Cytoplasm; Cell membrane; Golgi apparatus
Recommended Products
Secondary Antibody
Isotype Control
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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IHC Kits & Antibodies
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2498 | Hi-Affi™ Recombinant Rabbit Anti-NRAS Monoclonal Antibody (DS2498AB) | WB, IP | IgG |
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