Rabbit Anti-NRAS Recombinant Antibody (VS3-CJ235)
CAT#: VS3-CJ235
This product is a rabbit antibody that recognizes NRAS.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/79912/1987_E10_1_selected.jpg
Normal Tissue
Figure 2 IHC staining of human pancreas
Immunohistochemical staining of human pancreas shows strong cytoplasmic positivity in exocrine glandular cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/10157/ihc_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/49830/118215_B_7_5.jpg
Normal Tissue
Figure 4 Colon
Endothelial cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/49830/118215_A_9_3.jpg
Normal Tissue
Figure 5 Lymph node
Germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear Non-germinal center cells Staining: Not detected Intensity: Weak Quantity: <25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/49830/118215_A_8_8.jpg
RNA Expression
Figure 6 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000213281-NRAS
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Specifications
- Immunogen
- Recombinant protein
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse, Rat and Zebrafish NRAS
- Species Reactivity
- Human, Mouse, Rat, Zebrafish
- Applications
- WB, IP, FC
- Conjugate
- Unconjugated
Product Property
- Purification
- Protein A affinity purified
- Purity
- >95% as determined by SDS-PAGE
- Buffer
- 40% Glycerol, 1% BSA, TBS, pH7.4.
- Preservative
- 0.05% sodium azide
- Storage
- Store at 4°C for short term. Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- This antibody has been tested for use in Western Blot, Immunoprecipitation, Flow Cytometry.
Target
- Alternative Names
- NS6; CMNS; NCMS; ALPS4; N-ras; NRAS1; HRAS; KRAS
- Gene ID
- 4893
- UniProt ID
- P01111
- Sequence Similarities
- Belongs to the small GTPase superfamily. Ras family.
- Cellular Localization
- Cell membrane, Golgi apparatus, Membrane
- Post Translation Modifications
- Palmitoylated by the ZDHHC9-GOLGA7 complex (PubMed:16000296).
Depalmitoylated by ABHD17A, ABHD17B and ABHD17C (PubMed:26701913).
A continuous cycle of de- and re-palmitoylation regulates rapid exchange between plasma membrane and Golgi (PubMed:16000296, PubMed:15705808, PubMed:2661017, PubMed:26701913).
Acetylation at Lys-104 prevents interaction with guanine nucleotide exchange factors (GEFs).
Fatty-acylated at Lys-169 and/or Lys-170.
Ubiquitinated by the BCR(LZTR1) E3 ubiquitin ligase complex at Lys-170 in a non-degradative manner, leading to inhibit Ras signaling by decreasing Ras association with membranes.
Phosphorylation at Ser-89 by STK19 enhances NRAS-association with its downstream effectors.
(Microbial infection) Glucosylated at Thr-35 by P.sordellii toxin TcsL.
- Protein Refseq
- NP_002515.1
- Function
- Ras proteins bind GDP/GTP and possess intrinsic GTPase activity.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "NRAS"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1454CT | Recombinant Mouse anti-Human NRAS Monoclonal antibody (BU3H0) | ELISA, WB | |
| MRO-1321-CN | Recombinant Rabbit Anti-NRAS Monoclonal Antibody (CBACN-478) | WB, IP, FC | Rabbit IgG |
| MRO-1322-CN | Recombinant Rabbit Anti-NRAS Monoclonal Antibody (JF10-11) | WB, IF, IP | Rabbit IgG |
| VS3-CJ234 | Mouse Anti-NRAS Recombinant Antibody (VS3-CJ234) | WB | Mouse IgG1 |
| VS3-FY1069 | Recombinant Rabbit Anti-NRAS Antibody (clone R09-7E8) | WB, ICC, IF, IP | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2498 | Hi-Affi™ Recombinant Rabbit Anti-NRAS Monoclonal Antibody (DS2498AB) | WB, IP | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHC-YF595 | A*01:01/Human N-RAS (ILDTAGREEY) MHC Pentamer | FCM |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0424-XY205 | AbPlus™ Anti-NRAS Magnetic Beads (JF10-11) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1518 | Anti-NRAS Immunohistochemistry Kit | IHC | |
| VS-0525-XY4973 | Anti-Mouse NRAS Immunohistochemistry Kit | IHC | |
| VS-0525-XY4972 | Anti-Human NRAS Immunohistochemistry Kit | IHC |
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