Rabbit Anti-NRAS Recombinant Antibody (VS3-CJ235) (CAT#: VS3-CJ235)
This product is a rabbit antibody that recognizes NRAS.
Specific Inquiry
Custom Production
We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.
We require custom production
Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/79912/1987_E10_1_selected.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/79912/1987_E10_1_selected.jpg
Figure 2 IHC staining of human pancreas
(Immunohistochemical staining of human pancreas shows strong cytoplasmic positivity in exocrine glandular cells.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/10157/ihc_selected.jpg
(Immunohistochemical staining of human pancreas shows strong cytoplasmic positivity in exocrine glandular cells.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/10157/ihc_selected.jpg
Figure 3 Cerebral cortex
(Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/49830/118215_B_7_5.jpg
(Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/49830/118215_B_7_5.jpg
Figure 4 Colon
(Endothelial cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/49830/118215_A_9_3.jpg
(Endothelial cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/49830/118215_A_9_3.jpg
Figure 5 Lymph node
(Germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear Non-germinal center cells Staining: Not detected Intensity: Weak Quantity: <25% Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/49830/118215_A_8_8.jpg
(Germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear Non-germinal center cells Staining: Not detected Intensity: Weak Quantity: <25% Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/49830/118215_A_8_8.jpg
Figure 6 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000213281-NRAS
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000213281-NRAS
Specifications
- Immunogen
- Recombinant protein
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse, Rat and Zebrafish NRAS
- Species Reactivity
- Human, Mouse, Rat, Zebrafish
- Applications
- WB, IP, FC
- Conjugate
- Unconjugated
Product Property
- Purification
- Protein A affinity purified
- Purity
- >95% as determined by SDS-PAGE
- Buffer
- 40% Glycerol, 1% BSA, TBS, pH7.4.
- Preservative
- 0.05% sodium azide
- Storage
- Store at 4°C for short term. Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- This antibody has been tested for use in Western Blot, Immunoprecipitation, Flow Cytometry.
Target
- Alternative Names
- NS6; CMNS; NCMS; ALPS4; N-ras; NRAS1; HRAS; KRAS
- Gene ID
- 4893
- UniProt ID
- P01111
- Sequence Similarities
- Belongs to the small GTPase superfamily. Ras family.
- Cellular Localization
- Cell membrane, Golgi apparatus, Membrane
- Post Translation Modifications
- Palmitoylated by the ZDHHC9-GOLGA7 complex (PubMed:16000296).
Depalmitoylated by ABHD17A, ABHD17B and ABHD17C (PubMed:26701913).
A continuous cycle of de- and re-palmitoylation regulates rapid exchange between plasma membrane and Golgi (PubMed:16000296, PubMed:15705808, PubMed:2661017, PubMed:26701913).
Acetylation at Lys-104 prevents interaction with guanine nucleotide exchange factors (GEFs).
Fatty-acylated at Lys-169 and/or Lys-170.
Ubiquitinated by the BCR(LZTR1) E3 ubiquitin ligase complex at Lys-170 in a non-degradative manner, leading to inhibit Ras signaling by decreasing Ras association with membranes.
Phosphorylation at Ser-89 by STK19 enhances NRAS-association with its downstream effectors.
(Microbial infection) Glucosylated at Thr-35 by P.sordellii toxin TcsL.
- Protein Refseq
- NP_002515.1
- Function
- Ras proteins bind GDP/GTP and possess intrinsic GTPase activity.
Recommended Products
Isotype Control
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "NRAS"
Select a product category from the dropdown menu below to view related products.
Please select product type
Rabbit Monoclonal Antibodies
IgG Antibodies
MHC Tetramers for Cancer
Antibody Magnetic Beads
IHC Kits & Antibodies
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2498 | Hi-Affi™ Recombinant Rabbit Anti-NRAS Monoclonal Antibody (DS2498AB) | WB, IP | IgG |
Customer Reviews and Q&As
There are currently no Customer reviews or questions for VS3-CJ235. Click the button above to contact us or submit your feedback about this product.
Popular products with customers
Application: FuncS, IF, Neut, ELISA, FC, IP, ICC
Application: ELISA, Neut, IF, IP, FC, FuncS
Application: WB, FuncS, IF, Neut, ELISA, FC, IP
Application: FC, IP, ELISA, Neut, FuncS, IF, ICC
Application: Neut, ELISA, IF, IP, FuncS, FC, WB
Application: FC, IP, ELISA, Neut, FuncS, IF, WB
Application: FuncS, IF, Neut, ELISA, FC, IP, IHC
-2.png)
Application: WB, ELISA, FC, IHC, IP
Application: Neut, FC
Application: WB, ELISA, FuncS, IB, FC, SPR, Apop
Application: ELISA, FuncS
Application: ELISA, Cyt, PP, Inhib
For Research Use Only. Not For Clinical Use.
Send Inquiry
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
