Rabbit Anti-NSDHL Recombinant Antibody (clone R04-6I5)
CAT#: VS3-FY2053
The anti-NSDHL antibody can be tested in western blot analysis of NSDHL in Hela, A549, HL-60, U251 lysates. The recombinant monoclonal antibody specifically immunoreacts with human NSDHL. It is suitable for use in a variety of assays, including WB, IP.
Gene Expression
Tested Data
Subcellular Location
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to endoplasmic reticulum & lipid droplets.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/248/24_C1_1_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to endoplasmic reticulum & lipid droplets.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/571/25_C4_1_selected.jpg
Normal Tissue
Figure 3 Testis
Leydig cells Staining: High Intensity: Strong Quantity:>75% Sertoli cells Staining: Medium Intensity: Moderate Quantity:>75%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/571/1851_A_4_6.jpg
Normal Tissue
Figure 4 Epididymis
Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/571/1851_A_3_6.jpg
Normal Tissue
Figure 5 Lymph node
Germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/571/1851_A_8_8.jpg
RNA Expression
Figure 6 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000147383-NSDHL
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WB
Figure 1 Anti-NSDHL Antibody (VS3-FY2053) in WB.
The NSDHL expression in Hela, A549, HL-60, U251 lysates were detected using the anti-NSDHL by western blot assay.
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Specifications
- Immunogen
- Synthetic peptide of human NSDHL
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human NSDHL
- Species Reactivity
- Human
- Clone
- R04-6I5
- Applications
- Western Blot, Immunoprecipitation
- MW
- The calculated weight of the target protein is approximately 42 kDa, while the observed molecular weight stands at 42 kDa.
- Related Disease
- Congenital Hemidysplasia With Ichthyosiform Erythroderma And Limb Defects and Ck Syndrome
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunoprecipitation: 1/20
Target
- Alternative Names
- NAD(P) Dependent Steroid Dehydrogenase-Like; Short Chain Dehydrogenase/Reductase Family 31E, Member 1; Protein H105e3; EC 1.1.1.170; H105E3; Sterol-4-Alpha-Carboxylate 3-Dehydrogenase, Decarboxylating; SDR31E1; XAP104
- Gene ID
- 50814
- UniProt ID
- Q15738
- Sequence Similarities
- Belongs to the 3-beta-HSD family.
- Cellular Localization
- Endoplasmic reticulum, Lipid droplet, Membrane
- Protein Refseq
- NP_001123237.1; NP_057006.1
- Function
- Catalyzes the NAD(P)⁺-dependent oxidative decarboxylation of the C4 methyl groups of 4-alpha-carboxysterols in post-squalene cholesterol biosynthesis (By similarity).
Also plays a role in the regulation of the endocytic trafficking of EGFR (By similarity).
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "NSDHL"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1463CT | Recombinant Mouse anti-Human NSDHL Monoclonal antibody (EML145) | WB | |
| VS4-CJ60 | Recombinant Rabbit Anti-NSDHL Antibody (clone 20F2) | ELISA, WB, IHC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY4995 | Anti-NSDHL Immunohistochemistry Kit | IHC | |
| VS-0525-XY4996 | Anti-Mouse NSDHL Immunohistochemistry Kit | IHC |
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