Recombinant Rabbit Anti-NSDHL Antibody (clone 20F2) (CAT#: VS4-CJ60)
This rabbit anti-NSDHL antibody (clone 20F2) is a product that recognizes human NSDHL. The immunogen of this antibody is a synthesized peptide derived from human NSDHL. The mAb has been tested for use in ELISA, Western Blot (1:500-1:2000), Immunohistochemistry (1:50-1:200).
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Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line A-431
(Immunofluorescent staining of human cell line A-431 shows localization to endoplasmic reticulum & lipid droplets.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/248/24_C1_1_selected.jpg
(Immunofluorescent staining of human cell line A-431 shows localization to endoplasmic reticulum & lipid droplets.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/248/24_C1_1_selected.jpg
Figure 2 IF staining of human cell line U-251 MG
(Immunofluorescent staining of human cell line U-251 MG shows localization to endoplasmic reticulum & lipid droplets.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/571/25_C4_1_selected.jpg
(Immunofluorescent staining of human cell line U-251 MG shows localization to endoplasmic reticulum & lipid droplets.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/571/25_C4_1_selected.jpg
Figure 3 Testis
(Leydig cells Staining: High Intensity: Strong Quantity:>75% Sertoli cells Staining: Medium Intensity: Moderate Quantity:>75%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/571/1851_A_4_6.jpg
(Leydig cells Staining: High Intensity: Strong Quantity:>75% Sertoli cells Staining: Medium Intensity: Moderate Quantity:>75%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/571/1851_A_4_6.jpg
Figure 4 Epididymis
(Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/571/1851_A_3_6.jpg
(Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/571/1851_A_3_6.jpg
Figure 5 Lymph node
(Germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/571/1851_A_8_8.jpg
(Germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/571/1851_A_8_8.jpg
Figure 6 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000147383-NSDHL
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000147383-NSDHL
Specifications
- Immunogen
- A synthesized peptide derived from human NSDHL
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human NSDHL
- Species Reactivity
- Human
- Clone
- 20F2
- Applications
- ELISA, WB, IHC
- Conjugate
- Unconjugated
Product Property
- Purification
- Affinity chromatography
- Purity
- >95% as determined by SDS-PAGE
- Format
- Liquid
- Buffer
- 50% Glycerol, 150 mM NaCl, PBS, pH 7.4.
- Preservative
- 0.02% Sodium Azide
- Storage
- Store at -20°C or -80°C upon receipt. Avoid repeated freeze.
Applications
- Application Notes
- This antibody has been tested for use in ELISA, Western Blot (1:500-1:2000), Immunohistochemistry (1:50-1:200).
Target
- Alternative Names
- H105E3; XAP104; SDR31E1
- Gene ID
- 50814
- UniProt ID
- Q15738
- Long Name
- NAD(P) dependent steroid dehydrogenase-like
- Sequence Similarities
- Belongs to the 3-beta-HSD family.
- Cellular Localization
- Endoplasmic reticulum, Lipid droplet, Membrane
- Protein Refseq
- NP_001123237.1; NP_057006.1
- Function
- Catalyzes the NAD(P)+-dependent oxidative decarboxylation of the C4 methyl groups of 4-alpha-carboxysterols in post-squalene cholesterol biosynthesis (By similarity).
Also plays a role in the regulation of the endocytic trafficking of EGFR (By similarity).
Recommended Products
Isotype Control
Secondary Antibody
Recommended Dilution Buffer
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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IgG Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1463CT | Recombinant Mouse anti-Human NSDHL Monoclonal antibody (EML145) | WB | |
| VS3-FY2053 | Rabbit Anti-NSDHL Recombinant Antibody (clone R04-6I5) | WB, IP | Rabbit IgG |
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