Recombinant Rabbit Anti-NSDHL Antibody (clone 20F2) (CAT#: VS4-CJ60)
This rabbit anti-NSDHL antibody (clone 20F2) is a product that recognizes human NSDHL. The immunogen of this antibody is a synthesized peptide derived from human NSDHL. The mAb has been tested for use in ELISA, Western Blot (1:500-1:2000), Immunohistochemistry (1:50-1:200).
Specific Inquiry
Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line A-431
(Immunofluorescent staining of human cell line A-431 shows localization to endoplasmic reticulum & lipid droplets.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/248/24_C1_1_selected.jpg
(Immunofluorescent staining of human cell line A-431 shows localization to endoplasmic reticulum & lipid droplets.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/248/24_C1_1_selected.jpg
Figure 2 IF staining of human cell line U-251 MG
(Immunofluorescent staining of human cell line U-251 MG shows localization to endoplasmic reticulum & lipid droplets.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/571/25_C4_1_selected.jpg
(Immunofluorescent staining of human cell line U-251 MG shows localization to endoplasmic reticulum & lipid droplets.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/571/25_C4_1_selected.jpg
Figure 3 Testis
(Leydig cells Staining: High Intensity: Strong Quantity:>75% Sertoli cells Staining: Medium Intensity: Moderate Quantity:>75%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/571/1851_A_4_6.jpg
(Leydig cells Staining: High Intensity: Strong Quantity:>75% Sertoli cells Staining: Medium Intensity: Moderate Quantity:>75%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/571/1851_A_4_6.jpg
Figure 4 Epididymis
(Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/571/1851_A_3_6.jpg
(Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/571/1851_A_3_6.jpg
Figure 5 Lymph node
(Germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/571/1851_A_8_8.jpg
(Germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/571/1851_A_8_8.jpg
Figure 6 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000147383-NSDHL
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000147383-NSDHL
Specifications
- Immunogen
- A synthesized peptide derived from human NSDHL
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human NSDHL
- Species Reactivity
- Human
- Clone
- 20F2
- Applications
- ELISA, WB, IHC
- Conjugate
- Unconjugated
Product Property
- Purification
- Affinity chromatography
- Purity
- >95% as determined by SDS-PAGE
- Format
- Liquid
- Buffer
- 50% Glycerol, 150 mM NaCl, PBS, pH 7.4.
- Preservative
- 0.02% Sodium Azide
- Storage
- Store at -20°C or -80°C upon receipt. Avoid repeated freeze.
Applications
- Application Notes
- This antibody has been tested for use in ELISA, Western Blot (1:500-1:2000), Immunohistochemistry (1:50-1:200).
Target
- Alternative Names
- H105E3; XAP104; SDR31E1
- Gene ID
- 50814
- UniProt ID
- Q15738
- Long Name
- NAD(P) dependent steroid dehydrogenase-like
- Sequence Similarities
- Belongs to the 3-beta-HSD family.
- Cellular Localization
- Endoplasmic reticulum, Lipid droplet, Membrane
- Protein Refseq
- NP_001123237.1; NP_057006.1
- Function
- Catalyzes the NAD(P)+-dependent oxidative decarboxylation of the C4 methyl groups of 4-alpha-carboxysterols in post-squalene cholesterol biosynthesis (By similarity).
Also plays a role in the regulation of the endocytic trafficking of EGFR (By similarity).
Recommended Products
Isotype Control
Secondary Antibody
Recommended Dilution Buffer
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "NSDHL"
Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1463CT | Recombinant Mouse anti-Human NSDHL Monoclonal antibody (EML145) | WB |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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