Rabbit Anti-NUMA1 Recombinant Antibody (clone R05-2H8)
CAT#: VS3-XY3456
The recombinant rabbit antibody is specific for NUMA1 (Nuclear mitotic apparatus protein 1), which plays a role in the establishment of the mitotic spindle orientation during metaphase. The antibody specifically reacts with human, mouse and rat NUMA1. It has been tested using Western Blot, Immunohistochemistry-Paraffin, Immunocytochemistry or Immunofluorescence. The recommended dilutions of Western Blot, Immunohistochemistry-Paraffin and Immunofluorescence is 1/500-1/1000, 1/50-1/100 and 1/50-1/200, respectively.
Gene Expression
Normal Tissue
Figure 1 Cerebral cortex
Endothelial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear Glial cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Neuronal cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/19859/45507_B_7_5.jpg
Normal Tissue
Figure 2 Colon
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/19859/45697_A_9_3.jpg
Normal Tissue
Figure 3 Pancreas
Exocrine glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Pancreatic endocrine cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/19859/45697_A_1_3.jpg
Normal Tissue
Figure 4 Kidney
Cells in glomeruli Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Cells in tubules Staining: High Intensity: Strong Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/19859/45697_A_8_5.jpg
Normal Tissue
Figure 5 Testis
Leydig cells Staining: High Intensity: Strong Quantity:>75% Pachytene spermatocytes Staining: Medium Intensity: Moderate Quantity:>75% Peritubular cells Staining: High Intensity: Strong Quantity:>75% Preleptotene spermatocytes Staining: High Intensity: Strong Quantity:>75% Sertoli cells Staining: High Intensity: Strong Quantity:>75% Spermatogonia cells Staining: High Intensity: Strong
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/19859/45697_A_5_6.jpg
Normal Tissue
Figure 6 Lymph node
Germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Non-germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/19859/45697_A_9_8.jpg
RNA Expression
Figure 7 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000137497-NUMA1
❮
❯
Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat NUMA1
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R05-2H8
- Applications
- WB, IHC-P, ICC, IF
- Related Disease
- Acute Promyelocytic Leukemia; Bladder Calculus
Product Property
- Clonality
- Monoclonal
- Aggregation
- < 5%
- Format
- Liquid
- Buffer
- PBS (pH 7.4), 150 mM NaCl and 50% glycerol
- Preservative
- 0.02% sodium azide
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- WB: 1/500-1/1000
IHC: 1/50-1/100
IF: 1/50-1/200
Target
- Alternative Names
- NUMA; NMP-22
- Gene ID
- 4926
- UniProt ID
- Q14980
- Long Name
- Nuclear mitotic apparatus protein 1
- Cellular Localization
- Nucleus, nucleoplasm, Chromosome
- Post Translation Modifications
- Phosphorylation and dephosphorylation on Thr-2055 regulates the extent of cortical NUMA1 and the dynein-dynactin complex localization during mitotic metaphase and anaphase (PubMed:23921553).
In metaphase, phosphorylation on Thr-2055 oCcurs in a kinase CDK1-dependent manner; this phosphorylation maintains low levels of cortical dynein-dynactin complex at metaphase, and hence proper spindle positioning.
- Protein Refseq
- XP_011543356.1
- Function
- Microtubule (MT)-binding protein that plays a role in the formation and maintenance of the spindle poles and the alignement and the segregation of chromosomes during mitotic cell division.
Functions to tether the minus ends of MTs at the spindle poles, which is critical for the establishment and maintenance of the spindle poles (PubMed:12445386, 11956313).
Plays a role in the establishment of the mitotic spindle orientation during metaphase and elongation during anaphase in a dynein-dynactin-dependent manner.
REVIEWS AND Q&AS
CITATIONS
RESOURCES
DOWNLOADS
RELATED PRODUCTS
Inquiry
Navs
Customer Review
There are currently no Customer reviews or questions for VS3-XY3456. Click the button above to contact us or submit your feedback about this product.
Submit Your Publication
Published with our product? Submit your paper and receive a 10% discount on your next order! Share your research to earn exclusive rewards.
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Recommended Dilution Buffer
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "NUMA1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1866z | Mouse Anti-NUMA1 Recombinant Antibody (clone 1B8) | WB, ELISA, IHC, IP | Mouse IgG1, κ |
| MOB-2211MZ | Recombinant Mouse Anti-Human NUMA1 Antibody | ICC, IF, WB | Mouse antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2519 | Hi-Affi™ Recombinant Rabbit Anti-NUMA1 Monoclonal Antibody (DS2519AB) | WB, IHC-P, ICC, IF | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY5044 | Anti-NUMA1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY5045 | Anti-Mouse NUMA1 Immunohistochemistry Kit | IHC |
Specific Inquiry
Add to compare
See Our Custom Production in Action
Popular Products
Application: IF, IP, Neut, FuncS, ELISA, FC, WB
Application: ELISA, FC, IP, FuncS, IF, Neut, ICC
Application: Neut, ELISA, FuncS
Application: ELISA
-2.jpg)
Application: IB, ELISA, FC, FuncS
-3.png)
Application: ELISA, FC, Block, SPR
Application: IHC, ELISA, FC, WB, ADCC, FuncS
Application: ELISA, IHC, FC, IP, IF, FuncS
Application: ELISA
Application: WB, IHC-Fr, IHC-P, ICC, IF
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
Send Inquiry
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
