Mouse Anti-NUP98 Recombinant Antibody (VS3-FY2826) (CAT#: VS3-FY2826)

• Host Species
• Mouse

• Type
• Mouse IgG

• Specificity
• Human and Monkey NUP98

• Species Reactivity
• Human, Monkey

• Applications
• Western Blot, Immunocytochemistry, Flow cytometry, ELISA

• Conjugate
• Unconjugated, FITC

• MW
• The molecular weight of the target protein is approximately 197.5 kDa.

• Related Disease
• Acute Megakaryoblastic Leukemia Without Down Syndrome and Childhood Acute Myeloid Leukemia

• Clonality
• Monoclonal Antibody

• Format
• Liquid

• Concentration
• Please refer to the vial label for the specific concentration.

• Storage
• Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.

• Application Notes
• Western Blot: 1/500-1/2000
  Immunocytochemistry: 1/50-1/200
  Flow cytometry: 1/50-1/200
  ELISA: 1/1000-1/10000

• Alternative Names
• Nucleoporin 98; Nucleoporin 98kDa; Nuclear Pore Complex Protein Nup98-Nup96; GLFG-Repeat Containing Nucleoporin; Nuclear Pore Complex Protein Nup98; NUP98/PHF23 Fusion 2 Protein; Nucleoporin 98kD

• Gene ID
• 4928

• UniProt ID
• P52948

• Sequence Similarities
• Belongs to the nucleoporin GLFG family.

• Cellular Localization
• Nucleus membrane
  Peripheral membrane protein
  Nucleoplasmic side
  Nucleus, nuclear pore complex
  Nucleus, nucleoplasm

• Post Translation Modifications
• Isoform 1 to isoform 4 are autoproteolytically cleaved to yield Nup98 and Nup96 or Nup98 only, respectively (PubMed:10087256, 20407419, 12191480, 18287282).
  Cleaved Nup98 is necessary for the targeting of Nup98 to the nuclear pore and the interaction with Nup96 (PubMed:20407419, 12191480).
  (P52948-NUP98_HUMAN)
  Proteolytically degraded after poliovirus (PV) infection; degradation is partial and NCP- and TPR-binding domains withstand degradation. (P52948-NUP98_HUMAN)
  Glycosylation at Asn606 (NX_P52948 [NX_P52948-1])
  Ubiquitination at Lys193, Lys534, Lys572, Lys603, Lys639, Lys757, Lys1019, Lys1040, Lys1052, Lys1222, Lys1341, Lys1405, Lys1601, Lys1608, Lys1668, Lys1722, and Lys1739 (NX_P52948 [NX_P52948-1])
  Modification sites at PhosphoSitePlus (P52948)
  Glycosylation from GlyConnect NUP98_HUMAN (1578, 2900)
  Glycosylation from GlyGen (P52948) 107 sites, 1 N-linked glycan (1 site), 2 O-linked glycans (106 sites)

• Protein Refseq
• NP_001352055.1; NP_001352056.1; NP_001352057.1; NP_001352058.1

• Function
• Plays a role in the nuclear pore complex (NPC) assembly and/or maintenance.
  NUP98 and NUP96 are involved in the bidirectional transport across the NPC (PubMed:33097660).
  May anchor NUP153 and TPR to the NPC.
  In cooperation with DHX9, plays a role in transcription and alternative splicing activation of a subset of genes (PubMed:28221134).
  Involved in the localization of DHX9 in discrete intranuclear foci (GLFG-body) (PubMed:28221134). NUP98_HUMAN,P52948
  (Microbial infection) Interacts with HIV-1 capsid protein P24 and nucleocapsid protein P7 and may thereby promote the integration of the virus in the host nucleus (in vitro) (PubMed:23523133).
  Binding affinity to HIV-1 CA-NC complexes bearing the capsid change Asn-74-Asp is reduced (in vitro) (PubMed:23523133).

Secondary Antibody

• 2ND-040CQ
• Goat Anti-Mouse IgG (H&L) Antibody

Isotype Control

• MRO-A1591-YJ
• Mouse IgG Isotype control, Polyclonal

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