Rabbit Anti-NUP98 Recombinant Antibody (clone 2H4) (CAT#: ZG-0018U)
The purified Rabbit Anti-NUP98 Recombinant Antibody (clone 2H4) displayed high specificity against the NUP98 protein of human.
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WB
Figure 1 Rabbit Anti-NUP98 Antibody (ZG-0018U) in WB
Positive WB detected in: K562 whole cell lysate, Hela whole cell lysate. All lanes: NUP98 antibody at 1: 1000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 198, 188, 98, 97, 196, 187 kDa
Observed band size: 100 kDa
IHC
Figure 2 Rabbit Anti-NUP98 Antibody (ZG-0018U) in IHC
IHC image of ZG-0018U diluted at 1:100 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
IF
Figure 3 Rabbit Anti-NUP98 Antibody (ZG-0018U) in IF
Immunofluorescence staining of Hela Cells with ZG-0018U at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated Goat Anti-Rabbit IgG (H+L).
Specifications
- Immunogen
- A synthesized peptide derived from human NUP98
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human NUP98
- Species Reactivity
- Human
- Clone
- 2H4
- Applications
- ELISA, WB, IHC, IF
- Conjugate
- Unconjugated
- Related Disease
- Acute Megakaryoblastic Leukemia Without Down Syndrome and Childhood Acute Myeloid Leukemia
Product Property
- Purification
- Affinity purified
- Concentration
- See the COA
- Buffer
- PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
- Preservative
- 0.02% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Enzyme-linked Immunosorbent Assay, western blot, Immunohistochemistry, Immunofluorescence. Specific conditions should be optimized by the end user.
WB:1:500-1:5000, IHC:1:50-1:200, IF:1:20-1:200
Target
- Alternative Names
- Nuclear pore complex protein Nup98-Nup96; EC 3.4.21; Cleaved into: Nuclear pore complex protein Nup98;98 kDa nucleoporin; Nucleoporin Nup98; Nup98; Nuclear pore complex protein Nup96; 96 kDa nucleoporin; Nucleoporin Nup96; Nup96; NUP98; ADAR2
- Gene ID
- 4928
- UniProt ID
- P52948
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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See other products for "NUP98"
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IgG Antibody Products
Single Domain Antibody Products
Rabbit Monoclonal Antibody Products
Non-Human Primate (NHP) Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2810z | Mouse Anti-NUP98 Recombinant Antibody (clone 32A9) | WB, IP | Mouse IgG1 |
| ZG-0323C | Mouse Anti-NUP98 Recombinant Antibody (ZG-0323C) | WB | Mouse IgG |
| ZG-0324C | Mouse Anti-NUP98 Recombinant Antibody (ZG-0324C) | WB | Mouse IgG |
| VS7-0425-WR516 | Mouse Anti-NUP98 Recombinant Antibody (clone 2F5H8) | WB, FC | Mouse IgG1 |
| VS7-0425-WR517 | Mouse Anti-NUP98 Recombinant Antibody (clone 3G8C7) | WB, ICC, FC | Mouse IgG1 |
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For Research Use Only. Not For Clinical Use.
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