Rabbit Anti-NUP98 Recombinant Antibody (clone 2H4) (CAT#: ZG-0018U)
The purified Rabbit Anti-NUP98 Recombinant Antibody (clone 2H4) displayed high specificity against the NUP98 protein of human.
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WB
Figure 1 Rabbit Anti-NUP98 Antibody (ZG-0018U) in WB
Positive WB detected in: K562 whole cell lysate, Hela whole cell lysate. All lanes: NUP98 antibody at 1: 1000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 198, 188, 98, 97, 196, 187 kDa
Observed band size: 100 kDa
IHC
Figure 2 Rabbit Anti-NUP98 Antibody (ZG-0018U) in IHC
IHC image of ZG-0018U diluted at 1:100 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
IF
Figure 3 Rabbit Anti-NUP98 Antibody (ZG-0018U) in IF
Immunofluorescence staining of Hela Cells with ZG-0018U at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated Goat Anti-Rabbit IgG (H+L).
Specifications
- Immunogen
- A synthesized peptide derived from human NUP98
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human NUP98
- Species Reactivity
- Human
- Clone
- 2H4
- Applications
- ELISA, WB, IHC, IF
- Conjugate
- Unconjugated
- Related Disease
- Acute Megakaryoblastic Leukemia Without Down Syndrome and Childhood Acute Myeloid Leukemia
Product Property
- Purification
- Affinity purified
- Concentration
- See the COA
- Buffer
- PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
- Preservative
- 0.02% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Enzyme-linked Immunosorbent Assay, western blot, Immunohistochemistry, Immunofluorescence. Specific conditions should be optimized by the end user.
WB:1:500-1:5000, IHC:1:50-1:200, IF:1:20-1:200
Target
- Alternative Names
- Nuclear pore complex protein Nup98-Nup96; EC 3.4.21; Cleaved into: Nuclear pore complex protein Nup98;98 kDa nucleoporin; Nucleoporin Nup98; Nup98; Nuclear pore complex protein Nup96; 96 kDa nucleoporin; Nucleoporin Nup96; Nup96; NUP98; ADAR2
- Gene ID
- 4928
- UniProt ID
- P52948
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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See other products for "NUP98"
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IgG Antibody Products
Single Domain Antibody Products
Rabbit Monoclonal Antibody Products
Non-Human Primate (NHP) Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2810z | Mouse Anti-NUP98 Recombinant Antibody (clone 32A9) | WB, IP | Mouse IgG1 |
| ZG-0323C | Mouse Anti-NUP98 Recombinant Antibody (ZG-0323C) | WB | Mouse IgG |
| VS3-FY2826 | Mouse Anti-NUP98 Recombinant Antibody (VS3-FY2826) | WB, ICC, FC, ELISA | Mouse IgG |
| VS7-0425-WR516 | Mouse Anti-NUP98 Recombinant Antibody (clone 2F5H8) | WB, FC | Mouse IgG1 |
| VS7-0425-WR517 | Mouse Anti-NUP98 Recombinant Antibody (clone 3G8C7) | WB, ICC, FC | Mouse IgG1 |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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