Rabbit Anti-NXF1 Recombinant Antibody (clone R05-1C3)

Tested Data
Gene Expression
Datasheet
MSDS
COA

ICC

Figure 1 Anti-NXF1 Antibody (VS3-FY1807) in ICC.

Figure 1 Anti-NXF1 Antibody (VS3-FY1807) in ICC.

Immunocytochemistry staining of NXF1 (green) in Jurkat using the anti-NXF1 antibody. Nuclear DNA was labeled with DAPI (shown in blue).

Figure 2 Anti-NXF1 Antibody (VS3-FY1807) in WB.

Figure 2 Anti-NXF1 Antibody (VS3-FY1807) in WB.

The NXF1 expression in K562 lysates were detected using the anti-NXF1 by western blot assay.

Subcellular Location

Figure 1 IF staining of human cell line MCF7
(Immunofluorescent staining of human cell line MCF7 shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/61593/1080_H2_2_selected.jpg

Normal Tissue

Figure 2 IHC staining of human rectum
(Immunohistochemical staining of human rectum shows strong nuclear positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/61593/ihc_selected.jpg

Figure 3 IHC staining of human duodenum
(Immunohistochemical staining of human duodenum shows strong nuclear positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/16327/ihc_selected.jpg

Figure 4 Cerebral cortex
(Endothelial cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Nuclear Glial cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Nuclear Neuronal cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/61593/145210_B_9_5.jpg

Figure 5 Colon
(Endothelial cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Peripheral nerve/ganglion Staining: High Intensity: Strong Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/61593/145210_A_7_3.jpg

Figure 6 Liver
(Cholangiocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: nuclear Hepatocytes Staining: High Intensity: Strong Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/61593/145210_A_8_4.jpg

Figure 7 Kidney
(Cells in glomeruli Staining: High Intensity: Strong Quantity: 75%-25% Location: Nuclear Cells in tubules Staining: High Intensity: Strong Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/61593/145210_A_7_5.jpg

Figure 8 Testis
(Leydig cells Staining: High Intensity: Strong Quantity: 75%-25% Pachytene spermatocytes Staining: High Intensity: Strong Quantity: 75%-25% Peritubular cells Staining: Medium Intensity: Moderate Quantity:>75% Preleptotene spermatocytes Staining: High Intensity: Strong Quantity:>75% Round or early spermatids Staining: High Intensity: Strong Quantity:>75% Sertoli cells Staining: High Intensity: Strong Quantity:>75% Spermatogonia cells Staining: High Intensity: Strong)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/61593/145210_A_5_6.jpg

Figure 9 Lymph node
(Germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Non-germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/61593/145210_A_9_8.jpg

RNA Expression

Figure 10 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000162231-NXF1

Datasheet

MSDS

Certificate of Analysis Lookup
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number

Specifications

Immunogen
Synthetic peptide of human NXF1

Host Species
Rabbit

Type
Rabbit IgG

Specificity
Human NXF1

Species Reactivity
Human

Clone
R05-1C3

Applications
Western Blot, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunofluorescence, Immunoprecipitation

MW
The calculated weight of the target protein is approximately 70 kDa, while the observed molecular weight stands at 70 kDa.

Related Disease
Anterior Horn Cell Disease and Lethal Congenital Contracture Syndrome 1

Product Property

Purification
Affinity Purified

Format
Liquid

Concentration
Please refer to the vial label for the specific concentration.

Storage
Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Alternative Names
Nuclear RNA Export Factor 1; Tip Associating Protein; Tip-Associated Protein; MRNA Export Factor TAP; TAP; Tip-Associating Protein; MEX67

Gene ID
10482

UniProt ID
Q9UBU9

Sequence Similarities
Belongs to the NXF family.

Cellular Localization
Cytoplasm, Nuclear pore complex, Nucleus

Post Translation Modifications
Ubiquitination at Lys117, Lys173, and Lys230 (NX_Q9UBU9 [NX_Q9UBU9-1])
Modification sites at PhosphoSitePlus (Q9UBU9)
Glycosylation from GlyGen (Q9UBU9) 2 sites, 1 O-linked glycan (2 sites)

Protein Refseq
XP_047282201.1; XP_047282202.1; XP_047282203.1; XP_054223434.1; XP_054223435.1

Function
The NXF1-NXT1 heterodimer is involved in the export of HSP70 mRNA in conjunction with ALYREF/THOC4 and THOC5 components of the TREX complex.

CAT	Product Name	Application	Type
MOB-2111MZ	Recombinant Mouse Anti-Human NXF1 Antibody (clone 64I9)	ELISA, ICC/IF, IP, WB	Mouse antibody
MRO-1123-CN	Recombinant Rabbit Anti-NXF1 Monoclonal Antibody (JE40-63)	WB, IF, IP	Rabbit IgG
VS3-WK343	Rabbit Anti-NXF1 Recombinant Antibody (clone JE40-63)	WB, ELISA	Rabbit IgG

Rabbit Anti-NXF1 Recombinant Antibody (clone R05-1C3) (CAT#: VS3-FY1807)

Specifications

Product Property

Applications

Target

Recommended Products

Isotype Control

Secondary Antibody

Product Notes

Downloads

See other products for "NXF1"

Recombinant Antibody

Rabbit Monoclonal Antibody

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