Rabbit Anti-NXF1 Recombinant Antibody (clone R05-1C3)
CAT#: VS3-FY1807
This clone R05-1C3 is a rabbit recombinant monoclonal antibody that specifically immunoreacts with human NXF1. It is suitable for use in a variety of assays, including WB, IHC-F, IHC-P, ICC/IF, IP. To validate the QC result of the antibody, we recommend a dilution for the antibody by western blot (dilution 1/500-1/1000), immunohistochemistry (dilution 1/50-1/100), immunofluorescence (dilution 1/50-1/200), immunoprecipitation (dilution 1/20).
Gene Expression
Tested Data
Subcellular Location
Figure 1 IF staining of human cell line MCF7
Immunofluorescent staining of human cell line MCF7 shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/61593/1080_H2_2_selected.jpg
Normal Tissue
Figure 2 IHC staining of human rectum
Immunohistochemical staining of human rectum shows strong nuclear positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/61593/ihc_selected.jpg
Normal Tissue
Figure 3 IHC staining of human duodenum
Immunohistochemical staining of human duodenum shows strong nuclear positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/16327/ihc_selected.jpg
Normal Tissue
Figure 4 Cerebral cortex
Endothelial cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Nuclear Glial cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Nuclear Neuronal cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/61593/145210_B_9_5.jpg
Normal Tissue
Figure 5 Colon
Endothelial cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Peripheral nerve/ganglion Staining: High Intensity: Strong Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/61593/145210_A_7_3.jpg
Normal Tissue
Figure 6 Liver
Cholangiocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: nuclear Hepatocytes Staining: High Intensity: Strong Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/61593/145210_A_8_4.jpg
Normal Tissue
Figure 7 Kidney
Cells in glomeruli Staining: High Intensity: Strong Quantity: 75%-25% Location: Nuclear Cells in tubules Staining: High Intensity: Strong Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/61593/145210_A_7_5.jpg
Normal Tissue
Figure 8 Testis
Leydig cells Staining: High Intensity: Strong Quantity: 75%-25% Pachytene spermatocytes Staining: High Intensity: Strong Quantity: 75%-25% Peritubular cells Staining: Medium Intensity: Moderate Quantity:>75% Preleptotene spermatocytes Staining: High Intensity: Strong Quantity:>75% Round or early spermatids Staining: High Intensity: Strong Quantity:>75% Sertoli cells Staining: High Intensity: Strong Quantity:>75% Spermatogonia cells Staining: High Intensity: Strong
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/61593/145210_A_5_6.jpg
Normal Tissue
Figure 9 Lymph node
Germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Non-germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/61593/145210_A_9_8.jpg
RNA Expression
Figure 10 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000162231-NXF1
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ICC
Figure 1 Anti-NXF1 Antibody (VS3-FY1807) in ICC.
Immunocytochemistry staining of NXF1 (green) in Jurkat using the anti-NXF1 antibody. Nuclear DNA was labeled with DAPI (shown in blue).
WB
Figure 2 Anti-NXF1 Antibody (VS3-FY1807) in WB.
The NXF1 expression in K562 lysates were detected using the anti-NXF1 by western blot assay.
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Specifications
- Immunogen
- Synthetic peptide of human NXF1
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human NXF1
- Species Reactivity
- Human
- Clone
- R05-1C3
- Applications
- Western Blot, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunofluorescence, Immunoprecipitation
- MW
- The calculated weight of the target protein is approximately 70 kDa, while the observed molecular weight stands at 70 kDa.
- Related Disease
- Anterior Horn Cell Disease and Lethal Congenital Contracture Syndrome 1
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry: 1/50-1/100
Immunofluorescence: 1/50-1/200
Immunoprecipitation: 1/20
Target
- Alternative Names
- Nuclear RNA Export Factor 1; Tip Associating Protein; Tip-Associated Protein; MRNA Export Factor TAP; TAP; Tip-Associating Protein; MEX67
- Gene ID
- 10482
- UniProt ID
- Q9UBU9
- Sequence Similarities
- Belongs to the NXF family.
- Cellular Localization
- Cytoplasm, Nuclear pore complex, Nucleus
- Post Translation Modifications
- Ubiquitination at Lys117, Lys173, and Lys230 (NX_Q9UBU9 [NX_Q9UBU9-1])
Modification sites at PhosphoSitePlus (Q9UBU9)
Glycosylation from GlyGen (Q9UBU9) 2 sites, 1 O-linked glycan (2 sites)
- Protein Refseq
- XP_047282201.1; XP_047282202.1; XP_047282203.1; XP_054223434.1; XP_054223435.1
- Function
- The NXF1-NXT1 heterodimer is involved in the export of HSP70 mRNA in conjunction with ALYREF/THOC4 and THOC5 components of the TREX complex.
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "NXF1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2111MZ | Recombinant Mouse Anti-Human NXF1 Antibody (clone 64I9) | ELISA, ICC, IF, IP, WB | Mouse antibody |
| MRO-1123-CN | Recombinant Rabbit Anti-NXF1 Monoclonal Antibody (JE40-63) | WB, IF, IP | Rabbit IgG |
| VS3-WK343 | Rabbit Anti-NXF1 Recombinant Antibody (clone JE40-63) | WB, ELISA | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2530 | Hi-Affi™ Recombinant Rabbit Anti-NXF1 Monoclonal Antibody (DS2530AB) | WB, IHC, ICC, IP | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC331 | AbPlus™ Anti-NXF1 Magnetic Beads (VS-0724-YC331) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1536 | Anti-NXF1 Immunohistochemistry Kit | IHC |
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