Mouse Anti-OTX2 Monoclonal Antibody (VS7-0425-WR528) (CAT#: VS7-0425-WR528)
This product is a mouse IgG antibody that binds to human OTX2. The antibody can be used for WB, IHC-P, IF, ICC, FC, and ELISA assays.
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Subcellular Location and Protein Expression
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line HAP1
(Immunofluorescent staining of human cell line HAP1 shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1123/1852_E4_16_cr5afd4342eb471_selected.jpg
(Immunofluorescent staining of human cell line HAP1 shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1123/1852_E4_16_cr5afd4342eb471_selected.jpg
Figure 2 IHC staining of human hippocampus
(Immunohistochemical staining of human hippocampus shows moderate nuclear positivity in neuronal cells.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/633/1033_B_7_6_selected.jpg
(Immunohistochemical staining of human hippocampus shows moderate nuclear positivity in neuronal cells.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/633/1033_B_7_6_selected.jpg
Figure 3 IF staining of human cell line SiHa
(Immunofluorescent staining of human cell line SiHa shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1123/1606_F1_5_red_green.jpg
(Immunofluorescent staining of human cell line SiHa shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1123/1606_F1_5_red_green.jpg
Figure 4 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1123/1614_F1_1_red_green.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1123/1614_F1_1_red_green.jpg
Figure 5 Cerebral cortex
(Neuronal cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/633/1033_B_7_5.jpg
(Neuronal cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/633/1033_B_7_5.jpg
Figure 6 RNA cell line category: Cell line enhanced (HAP1, hTERT-RPE1, NTERA-2)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000165588-OTX2
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000165588-OTX2
Specifications
- Immunogen
- Recombinant human OTX2 fragment expressed in E. Coli.
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human OTX2
- Species Reactivity
- Human
- Molecule Class
- Bicoid-Class Of Homeodomain-Containing Proteins
- Applications
- WB, IHC-P, IF, ICC, FC, ELISA
- Conjugate
- Unconjugated
- Related Disease
- Microphthalmia, syndromic, 5 (MCOPS5); Pituitary hormone deficiency, combined, 6 (CPHD6); Retinal dystrophy, early-onset, with or without pituitary dysfunction (RDEOP)
Product Property
- Clonality
- Monoclonal
- Purity
- >95% as determined by SDS-PAGE and SEC-HPLC analysis, Ascitic fluid
- Format
- Liquid
- Concentration
- 1 mg/ml
- Preservative
- 0.03% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Aliquot and store at -20°C for one year. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- Western Blot: 1:500 - 1:2000
Immunohistochemistry: 1:200 - 1:1000
Immunofluorescence: 1:200 - 1:1000
Enzyme-linked Immunosorbent Assay: 1:10000
Target
- Alternative Names
- OTX2; orthodenticle homeobox 2; CPHD6; MCOPS5; homeobox protein OTX2; orthodenticle homolog 2
- Gene ID
- 5015
- UniProt ID
- F1T0D1
- Long Name
- Orthodenticle Homeobox 2
- Cellular Localization
- nucleus
- Protein Refseq
- NP_001257452.1
- Function
- Developmental protein; DNA-binding
Recommended Products
Isotype Control
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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IgG Antibody Products
Rabbit Monoclonal Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| DrMAB-422 | Recombinant Human Anti-Human OTX2 Antibody, clone U36 | IP, IF | IgG |
| VS3-QX843 | Mouse Anti-OTX2 Recombinant Antibody (clone 1313CT817.185.162) | WB | Mouse IgG1, κ |
| VS3-QX844 | Mouse Anti-OTX2 Recombinant Antibody (clone 1H12C4B5) | WB, IHC, ICC, FC, ELISA | Mouse IgG1 |
| VS7-0425-WR526 | Mouse Anti-OTX2 Monoclonal Antibody (clone 1H12G8B2) | WB, IHC, ICC | Mouse IgG1 |
| VS7-0425-WR527 | Mouse Anti-OTX2 Monoclonal Antibody (VS7-0425-WR527) | WB, IHC-P, IF, ICC, ELISA | Mouse IgG |
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For Research Use Only. Not For Clinical Use.
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