Mouse Anti-OTX2 Monoclonal Antibody (VS7-0425-WR528) (CAT#: VS7-0425-WR528)
This product is a mouse IgG antibody that binds to human OTX2. The antibody can be used for WB, IHC-P, IF, ICC, FC, and ELISA assays.
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We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.
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Subcellular Location and Protein Expression
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line HAP1
(Immunofluorescent staining of human cell line HAP1 shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1123/1852_E4_16_cr5afd4342eb471_selected.jpg
(Immunofluorescent staining of human cell line HAP1 shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1123/1852_E4_16_cr5afd4342eb471_selected.jpg
Figure 2 IHC staining of human hippocampus
(Immunohistochemical staining of human hippocampus shows moderate nuclear positivity in neuronal cells.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/633/1033_B_7_6_selected.jpg
(Immunohistochemical staining of human hippocampus shows moderate nuclear positivity in neuronal cells.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/633/1033_B_7_6_selected.jpg
Figure 3 IF staining of human cell line SiHa
(Immunofluorescent staining of human cell line SiHa shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1123/1606_F1_5_red_green.jpg
(Immunofluorescent staining of human cell line SiHa shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1123/1606_F1_5_red_green.jpg
Figure 4 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1123/1614_F1_1_red_green.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1123/1614_F1_1_red_green.jpg
Figure 5 Cerebral cortex
(Neuronal cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/633/1033_B_7_5.jpg
(Neuronal cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/633/1033_B_7_5.jpg
Figure 6 RNA cell line category: Cell line enhanced (HAP1, hTERT-RPE1, NTERA-2)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000165588-OTX2
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000165588-OTX2
Specifications
- Immunogen
- Recombinant human OTX2 fragment expressed in E. Coli.
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human OTX2
- Species Reactivity
- Human
- Molecule Class
- Bicoid-Class Of Homeodomain-Containing Proteins
- Applications
- WB, IHC-P, IF, ICC, FC, ELISA
- Conjugate
- Unconjugated
- Related Disease
- Microphthalmia, syndromic, 5 (MCOPS5); Pituitary hormone deficiency, combined, 6 (CPHD6); Retinal dystrophy, early-onset, with or without pituitary dysfunction (RDEOP)
Product Property
- Clonality
- Monoclonal
- Purity
- >95% as determined by SDS-PAGE and SEC-HPLC analysis, Ascitic fluid
- Format
- Liquid
- Concentration
- 1 mg/ml
- Preservative
- 0.03% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Aliquot and store at -20°C for one year. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- Western Blot: 1:500 - 1:2000
Immunohistochemistry: 1:200 - 1:1000
Immunofluorescence: 1:200 - 1:1000
Enzyme-linked Immunosorbent Assay: 1:10000
Target
- Alternative Names
- OTX2; orthodenticle homeobox 2; CPHD6; MCOPS5; homeobox protein OTX2; orthodenticle homolog 2
- Gene ID
- 5015
- UniProt ID
- F1T0D1
- Long Name
- Orthodenticle Homeobox 2
- Cellular Localization
- nucleus
- Protein Refseq
- NP_001257452.1
- Function
- Developmental protein; DNA-binding
Recommended Products
Isotype Control
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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IgG Antibody Products
Rabbit Monoclonal Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| DrMAB-422 | Recombinant Human Anti-Human OTX2 Antibody, clone U36 | IP, IF | IgG |
| VS3-QX843 | Mouse Anti-OTX2 Recombinant Antibody (clone 1313CT817.185.162) | WB | Mouse IgG1, κ |
| VS3-QX844 | Mouse Anti-OTX2 Recombinant Antibody (clone 1H12C4B5) | WB, IHC, ICC, FC, ELISA | Mouse IgG1 |
| VS7-0425-WR526 | Mouse Anti-OTX2 Monoclonal Antibody (clone 1H12G8B2) | WB, IHC, ICC | Mouse IgG1 |
| VS7-0425-WR527 | Mouse Anti-OTX2 Monoclonal Antibody (VS7-0425-WR527) | WB, IHC-P, IF, ICC, ELISA | Mouse IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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