Rabbit Anti-PAWR Recombinant Antibody (clone R01-8C0)
CAT#: VS3-FY2058
The R01-8C0 recombinant monoclonal antibody with high affinity for human PAWR. It cross-reacts with mouse. It is suitable for use in a variety of assays, including WB, IP. The antibody has the positive detection of PAR4 protein by western blot analysis of PAR4 in 3T3 lysates.
Gene Expression
Tested Data
Subcellular Location
Figure 1 IF staining of human cell line SiHa
Immunofluorescent staining of human cell line SiHa shows localization to plasma membrane & actin filaments.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/12640/1183_B9_2_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane & actin filaments.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/20779/if_selected.jpg
Normal Tissue
Figure 3 Lymph node
Germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/12640/31196_A_9_8.jpg
Normal Tissue
Figure 4 Cerebral cortex
Endothelial cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Glial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Neuronal cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/12640/31196_B_7_5.jpg
Normal Tissue
Figure 5 Colon
Endothelial cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/12640/31196_A_7_3.jpg
Normal Tissue
Figure 6 Liver
Cholangiocytes Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Hepatocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/12640/31196_A_7_4.jpg
Normal Tissue
Figure 7 Kidney
Cells in glomeruli Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Cells in tubules Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/12640/31196_A_9_5.jpg
Normal Tissue
Figure 8 Testis
Cells in seminiferous ducts Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/12640/31196_A_5_6.jpg
RNA Expression
Figure 9 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000177425-PAWR
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WB
Figure 1 Anti-PAWR Antibody (VS3-FY2058) in WB.
The PAR4 expression in 3T3 lysates were detected using the anti-PAR4 by western blot assay.
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Specifications
- Immunogen
- Synthetic peptide of human PAR4
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human and Mouse PAWR
- Species Reactivity
- Human, Mouse
- Clone
- R01-8C0
- Applications
- Western Blot, Immunoprecipitation
- MW
- The calculated weight of the target protein is approximately 37 kDa, while the observed molecular weight stands at 41 kDa.
- Related Disease
- Cancer
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunoprecipitation: 1/20
Target
- Alternative Names
- Pro-Apoptotic WT1 Regulator; PRKC, Apoptosis, WT1, Regulator; Prostate Apoptosis Response-4; PAR-4; PAR4; Prostate Apoptosis Response Protein PAR-4
- Gene ID
- 5074
- UniProt ID
- Q96IZ0
- Cellular Localization
- Cytoplasm, Nucleus
- Post Translation Modifications
- Preferentially phosphorylated at the Thr-163 by PKC in cancer cells. (Q96IZ0-PAWR_HUMAN)
Ubiquitination at Lys333 (NX_Q96IZ0 [NX_Q96IZ0-1])
Modification sites at PhosphoSitePlus (Q96IZ0)
Glycosylation from GlyGen (Q96IZ0) 12 sites, 1 O-linked glycan (12 sites)
- Protein Refseq
- XP_016874867.1; XP_016874868.1; XP_047284872.1; XP_047284873.1; XP_047284874.1
- Function
- Induces apoptosis in certain cancer cells by activation of the Fas prodeath pathway and coparallel inhibition of NF-kappa-B transcriptional activity. Inhibits the transcriptional activation and augments the transcriptional repression mediated by WT1. Down-regulates the anti-apoptotic protein BCL2 via its interaction with WT1. Seems also to be a transcriptional repressor by itself. May be directly involved in regulating the amyloid precursor protein (APP) cleavage activity of BACE1.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "PAWR"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1548CT | Recombinant Mouse anti-Human PAWR Monoclonal antibody (4H0I8,5I23F0) | ELISA, IHC-P, WB | |
| ZG-0354C | Mouse Anti-PAWR Recombinant Antibody (ZG-0354C) | WB, IHC, ELISA | Mouse IgG |
| VS13-YC870 | Rabbit Anti-PAWR Recombinant Antibody (VS13-YC870) | WB | Rabbit IgG |
| VS7-0425-WR559 | Mouse Anti-PAWR Monoclonal Antibody (clone 3G9H7) | WB, IHC | Mouse IgG1 |
| VS7-0425-WR563 | Mouse Anti-PAWR Monoclonal Antibody (VS7-0425-WR563) | WB, IHC-P, IF, ELISA | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2599 | Hi-Affi™ Recombinant Rabbit Anti-PAWR Monoclonal Antibody (DS2599AB) | IP, WB | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1568 | Anti-PAWR Immunohistochemistry Kit | IHC | |
| VS-0525-XY5194 | Anti-Human PAWR Immunohistochemistry Kit | IHC |
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