Rabbit Anti-PDCD7 Recombinant Antibody (clone R47-1Q-5) (CAT#: VS3-FY2157)
This R47-1Q-5 recombinant monoclonal antibody has the ability to specifically bind to human PDCD7 protein, a calculated weight of 55 kDa. It cross-reacts with mouse, rat.
The following recommendations serve as a starting point for assay optimization. The actual working concentration may vary and should be determined by the user. The R47-1Q-5 is recommended for detection of target protein by western blotting (dilution range 1:500-1:1000), immunohistochemistry (dilution range 1:100-1:200), immunofluorescence (dilution range 1:50-1:200).
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Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/49388/if_selected.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/49388/if_selected.jpg
Figure 2 IHC staining of human kidney
(Immunohistochemical staining of human kidney shows strong cytoplasmic positivity in cells in tubules.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/49388/ihc_selected.jpg
(Immunohistochemical staining of human kidney shows strong cytoplasmic positivity in cells in tubules.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/49388/ihc_selected.jpg
Figure 3 Cerebral cortex
(Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/49388/112900_B_9_5.jpg
(Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/49388/112900_B_9_5.jpg
Figure 4 Colon
(Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/49388/112900_A_9_3.jpg
(Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/49388/112900_A_9_3.jpg
Figure 5 Liver
(Hepatocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/49388/112900_A_9_4.jpg
(Hepatocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/49388/112900_A_9_4.jpg
Figure 6 Kidney
(Cells in tubules Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/49388/112900_A_8_5.jpg
(Cells in tubules Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/49388/112900_A_8_5.jpg
Figure 7 Testis
(Elongated or late spermatids Staining: Medium Intensity: Moderate Quantity: 75%-25% Leydig cells Staining: High Intensity: Strong Quantity:>75% Pachytene spermatocytes Staining: Low Intensity: Moderate Quantity: <25%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/49388/112900_A_4_6.jpg
(Elongated or late spermatids Staining: Medium Intensity: Moderate Quantity: 75%-25% Leydig cells Staining: High Intensity: Strong Quantity:>75% Pachytene spermatocytes Staining: Low Intensity: Moderate Quantity: <25%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/49388/112900_A_4_6.jpg
Figure 8 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000090470-PDCD7
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000090470-PDCD7
Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat PDCD7
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R47-1Q-5
- Applications
- Western Blot, Immunohistochemistry-Paraffin, Immunofluorescence
- Conjugate
- Unconjugated
- MW
- The calculated weight of the target protein is approximately 55 kDa, while the observed molecular weight stands at 55kDa.
Product Property
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS (pH 7.4), 150 mM NaCl, 50% glycerol
- Preservative
- 0.02% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry: 1/100-1/200
Immunofluorescence: 1/50-1/200
Target
- Alternative Names
- Programmed Cell Death 7; U11/U12 SnRNP 59K; HES18; ES18; Programmed Cell Death Protein 7; Apoptosis-Related Protein ES18
- Gene ID
- 10081
- UniProt ID
- Q8N8D1
- Cellular Localization
- Nucleus
- Post Translation Modifications
- Ubiquitination at Lys280 (NX_Q8N8D1 [NX_Q8N8D1-1])
Modification sites at PhosphoSitePlus (Q8N8D1)
- Protein Refseq
- NP_005698.1
- Function
- Promotes apoptosis when overexpressed.
Recommended Products
Isotype Control
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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IgG Antibody Products
Rabbit Monoclonal Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1323CT | Recombinant Mouse anti-Mouse PDCD7 Monoclonal antibody (57D824) | WB |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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