CytoStream™ Rabbit Anti-PDHB Recombinant Antibody (VS13-YC885)
CAT#: VS13-YC885
The CytoStream™ Rabbit Anti-PDHB Recombinant Antibody is specific for human, mouse and rat PDHB. PDHB encodes a subunit of the pyruvate dehydrogenase complex, linking glycolysis and the TCA cycle, with associated gene mutations causing E1-beta deficiency, showing transcript diversity. The anti-PDHB antibody was developed and selected by our CytoStream™ platform and the mAb is recommended for use in flow cytometry staining (1/50-1/100).
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to mitochondria.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/36745/if_selected.jpg
Normal Tissue
Figure 2 Cerebral cortex
Glial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Neuronal cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/36744/77783_B_8_5.jpg
Normal Tissue
Figure 3 Colon
Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/36744/77783_A_9_3.jpg
Normal Tissue
Figure 4 Kidney
Collecting ducts Staining: High Intensity: Strong Quantity:>75% Distal tubules Staining: High Intensity: Strong Quantity:>75%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/36744/77783_A_7_5.jpg
Normal Tissue
Figure 5 Heart muscle
Cardiomyocytes Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/36744/77783_B_6_6.jpg
Normal Tissue
Figure 6 Lymph node
Non-germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/36744/77783_A_8_8.jpg
RNA Expression
Figure 7 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000168291-PDHB
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Specifications
- Immunogen
- Recombinant human pyruvate dehydrogenase E1 beta subunit, aa 310-359.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat PDHB
- Species Reactivity
- Human, Mouse, Rat
- Applications
- WB, IHC-P, FC, IP
- Conjugate
- NONE
- Related Disease
- Pyruvate Dehydrogenase E1-Beta Deficiency; Pyruvate Dehydrogenase E1-Alpha Deficiency
Product Property
- Clonality
- Monoclonal
- Purification
- Protein A purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- TBS (pH 7.4) containing 40% Glycerol and 0.05% BSA.
- Preservative
- 0.05% Sodium azide
- Storage
- Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Shipping with ice pack.
Applications
- Application Notes
- Western blot (WB): 1/500-1/2000
Immunohistochemistry-paraffin (IHC-P): 1/50-1/200
Flow cytometry (FC): 1/50-1/100
Target
- Alternative Names
- Pyruvate Dehydrogenase E1 Beta Subunit; Pyruvate Dehydrogenase (Lipoamide) Beta; EC 1.2.4.1; PDHE1-B; PHE1B; Pyruvate Dehydrogenase E1 Component Subunit Beta, Mitochondrial; Pyruvate Dehydrogenase, E1 Beta Polypeptide; PDHE1B; PDHBD;
- Gene ID
- 5162
- UniProt ID
- P11177
- Long Name
- Pyruvate Dehydrogenase E1 Beta Subunit
- Cellular Localization
- Mitochondrion matrix
- Protein Refseq
- NP_001166939.1; NP_000916.2
- Function
- The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2), and thereby links the glycolytic pathway to the tricarboxylic cycle.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "PDHB"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2827z | Mouse Anti-PDHB Recombinant Antibody (clone 8F7) | WB, ELISA, IHC | Mouse IgG1, κ |
| MOB-1735CT | Recombinant Mouse anti-Human PDHB Monoclonal antibody (EML611) | IHC-P, WB | |
| MRO-1302-CN | Recombinant Rabbit Anti-PDHB Monoclonal Antibody (JM66-33) | WB, IHC, IP, FC | Rabbit IgG |
| VS3-XY3459 | Rabbit Anti-PDHB Recombinant Antibody (clone R08-6I2) | WB, IHC-P, ICC, IF, IP, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2640 | Hi-Affi™ Recombinant Rabbit Anti-PDHB Monoclonal Antibody (DS2640AB) | WB, IHC-P, ICC, IF, FC | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1603 | Anti-PDHB Immunohistochemistry Kit | IHC | |
| VS-0525-XY5283 | Anti-Mouse PDHB Immunohistochemistry Kit | IHC | |
| VS-0525-XY5282 | Anti-Human PDHB Immunohistochemistry Kit | IHC |
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