Rabbit Anti-PDHB Recombinant Antibody (clone R08-6I2) (CAT#: VS3-XY3459)
This product is a recombinant rabbit antibody binding to PDHB (Pyruvate dehydrogenase E1 subunit beta). This antibody specifically recognizes human PDHB. It also has cross-reactivity against mouse and rat PDHB. It has been detected by Western Blot, Immunohistochemistry-Paraffin, Immunoprecipitation, Flow cytometry, Immunocytochemistry or Immunofluorescence. The recommended dilutions of Western Blot, Immunohistochemistry-Paraffin, Immunoprecipitation, Flow cytometry and Immunofluorescence is 1/500-1/1000, 1/50-1/100, 1/20, 1/50-1/100 and 1/50-1/200, respectively.
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Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to mitochondria.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/36745/if_selected.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to mitochondria.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/36745/if_selected.jpg
Figure 2 Cerebral cortex
(Glial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Neuronal cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/36744/77783_B_8_5.jpg
(Glial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Neuronal cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/36744/77783_B_8_5.jpg
Figure 3 Colon
(Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/36744/77783_A_9_3.jpg
(Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/36744/77783_A_9_3.jpg
Figure 4 Kidney
(Collecting ducts Staining: High Intensity: Strong Quantity:>75% Distal tubules Staining: High Intensity: Strong Quantity:>75%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/36744/77783_A_7_5.jpg
(Collecting ducts Staining: High Intensity: Strong Quantity:>75% Distal tubules Staining: High Intensity: Strong Quantity:>75%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/36744/77783_A_7_5.jpg
Figure 5 Heart muscle
(Cardiomyocytes Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/36744/77783_B_6_6.jpg
(Cardiomyocytes Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/36744/77783_B_6_6.jpg
Figure 6 Lymph node
(Non-germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/36744/77783_A_8_8.jpg
(Non-germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/36744/77783_A_8_8.jpg
Figure 7 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000168291-PDHB
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000168291-PDHB
Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat PDHB
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R08-6I2
- Applications
- WB, IHC-P, ICC, IF, IP, FC
- Related Disease
- Pyruvate Dehydrogenase E1-Beta Deficiency; Pyruvate Dehydrogenase E1-Alpha Deficiency
Product Property
- Clonality
- Monoclonal
- Aggregation
- < 5%
- Format
- Liquid
- Buffer
- PBS (pH 7.4), 150 mM NaCl and 50% glycerol
- Preservative
- 0.02% sodium azide
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- WB: 1/500-1/1000
IHC: 1/50-1/100
IF: 1/50-1/200
IP: 1/20
FC: 1/50-1/100
Target
- Alternative Names
- PDHBD; PHE1B; E1beta; PDHE1B; PDHE1-B
- Gene ID
- 5162
- UniProt ID
- P11177
- Long Name
- Pyruvate dehydrogenase E1 subunit beta
- Cellular Localization
- Mitochondrion matrix
- Post Translation Modifications
- Ubiquitination at Lys336 (NX_P11177 [NX_P11177-1])
Modification sites at PhosphoSitePlus (P11177)
Glycosylation from GlyGen (P11177) 2 sites, 1 O-linked glycan (2 sites)
- Protein Refseq
- NP_001166939.1; NP_000916.2
- Function
- The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2), and thereby links the glycolytic pathway to the tricarboxylic cycle.
Recommended Products
Isotype Control
Recommended Dilution Buffer
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
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| CAT | Product Name | Application | Type |
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| MOB-2827z | Mouse Anti-PDHB Recombinant Antibody (clone 8F7) | WB, ELISA, IHC | Mouse IgG1, κ |
| MOB-1735CT | Recombinant Mouse anti-Human PDHB Monoclonal antibody (EML611) | IHC-P, WB | |
| MRO-1302-CN | Recombinant Rabbit Anti-PDHB Monoclonal Antibody (JM66-33) | WB, IHC, IP, FC | Rabbit IgG |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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