Mouse Anti-PECAM1 Recombinant Antibody (clone PT0035)
CAT#: VS3-CJ697
This product is a mouse antibody that recognizes human PECAM1.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line THP-1
Immunofluorescent staining of human cell line THP-1 shows localization to nucleoli & plasma membrane.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4871/2005_B3_2_selected.jpg
Normal Tissue
Figure 2 Lung
High expression
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4690/13163_A_2_4_val_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Endothelial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4690/13163_B_8_5.jpg
Normal Tissue
Figure 4 Colon
Endothelial cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4690/13163_A_9_3.jpg
Normal Tissue
Figure 5 Kidney
Cells in glomeruli Staining: High Intensity: Strong Quantity: 75%-25%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4690/13163_A_8_5.jpg
RNA Expression
Figure 6 RNA cell line category: Group enriched (HUVEC TERT2, TIME)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000261371-PECAM1
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Specifications
- Immunogen
- Synthesized peptide derived from human CD31
- Host Species
- Mouse
- Type
- Mouse IgG1, κ
- Specificity
- Human PECAM1
- Species Reactivity
- Human
- Clone
- PT0035
- Applications
- WB, IHC-P
- Conjugate
- Unconjugated
Product Property
- Purification
- Affinity Chromatography
- Purity
- >95% as determined by SDS-PAGE
- Format
- Liquid
- Buffer
- 50% Glycerol, 0.5% BSA, PBS.
- Preservative
- 0.02% Sodium Azide
- Storage
- Store at 4°C for short term. Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Western Blot (1:500-1:2000), Immunohistochemistry-Parraffin (1:100-1:500).
Target
- Alternative Names
- CD31; PECA1; GPIIA'; PECAM-1; endoCAM; CD31/EndoCAM
- Gene ID
- 5175
- UniProt ID
- P16284
- Cellular Localization
- Cell junction, Cell membrane, Membrane
- Post Translation Modifications
- Phosphorylated on Ser and Tyr residues after cellular activation by src kinases (PubMed:21464369, PubMed:9298995, PubMed:19342684, PubMed:18710921).
Upon activation, phosphorylated on Ser-729 which probably initiates the dissociation of the membrane-interaction segment (residues 709-729) from the cell membrane allowing the sequential phosphorylation of Tyr-713 and Tyr-690 (PubMed:21464369).
Constitutively phosphorylated on Ser-734 in resting platelets (PubMed:21464369).
Phosphorylated on tyrosine residues by FER and FES in response to FCER1 activation (By similarity).
In endothelial cells Fyn mediates mechanical-force (stretch or pull) induced tyrosine phosphorylation (PubMed:18710921).
Palmitoylation by ZDHHC21 is necessary for cell surface expression in endothelial cells and enrichment in membrane rafts.
- Protein Refseq
- NP_000433.4
- Function
- Cell adhesion molecule which is required for leukocyte transendothelial migration (TEM) under most inflammatory conditions (PubMed:19342684, PubMed:17580308).
Tyr-690 plays a critical role in TEM and is required for efficient trafficking of PECAM1 to and from the lateral border recycling compartment (LBRC) and is also essential for the LBRC membrane to be targeted around migrating leukocytes (PubMed:19342684).
Trans-homophilic interaction may play a role in endothelial cell-cell adhesion via cell junctions (PubMed:27958302).
Heterophilic interaction with CD177 plays a role in transendothelial migration of neutrophils (PubMed:17580308).
Homophilic ligation of PECAM1 prevents macrophage-mediated phagocytosis of neighboring viable leukocytes by transmitting a detachment signal (PubMed:12110892).
Promotes macrophage-mediated phagocytosis of apoptotic leukocytes by tethering them to the phagocytic cells; PECAM1-mediated detachment signal appears to be disabled in apoptotic leukocytes (PubMed:12110892).
Modulates bradykinin receptor BDKRB2 activation (PubMed:18672896).
Regulates bradykinin- and hyperosmotic shock-induced ERK1/2 activation in endothelial cells (PubMed:18672896).
Induces susceptibility to atherosclerosis (By similarity).
Isoform Delta15: Does not protect against apoptosis.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Secondary Antibody
- CAT
- Product Name
Recommended Dilution Buffer
- CAT
- Product Name
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