Human Anti-PEG Recombinant Antibody (clone E11) (CAT#: FAMAB-1525CQ)

Figure 1 Immunoblot of free PEG and a PEG-modified enzyme.

Immunoblot of free PEG and a PEG-modified enzyme. Samples were electrophoresed on a 8% reduced SDS polyacrylamide gel, transferred to nitrocellulose paper, and probed with mAb E11. Lane 1, βG; lane 2, 3.5DNS-PEG3400-βG; lane 3, NH2-PEG1500-NH2; lane 4, CH3-PEG2000-OH. kDa, molecular mass in thousands.

Cheng, T. L., Cheng, C. M., Chen, B. M., Tsao, D. A., Chuang, K. H., Hsiao, S. W., ... & Roffler, S. R. (2005). Monoclonal antibody-based quantitation of poly (ethylene glycol)-derivatized proteins, liposomes, and nanoparticles. Bioconjugate chemistry, 16(5), 1225-1231.

Figure 2 Qualitative analysis of PEG-derivatized molecules.

Qualitative analysis of PEG-derivatized molecules. The binding of E11 to PEG and PEG-modified βG coated in microplates was detected by ELISA. The mean absorbance values (405 nm) of triplicate determinations are shown. Bars, SD.

Cheng, T. L., Cheng, C. M., Chen, B. M., Tsao, D. A., Chuang, K. H., Hsiao, S. W., ... & Roffler, S. R. (2005). Monoclonal antibody-based quantitation of poly (ethylene glycol)-derivatized proteins, liposomes, and nanoparticles. Bioconjugate chemistry, 16(5), 1225-1231.

Figure 3 Quantitative analysis of PEG-derivatized molecules by ELISA.

Graded concentrations of 3.5DNS-PEG3400-âG were assayed with a sandwich ELISA in which E11/AGP3 (●) or AGP3/E11 (○) were used for the capture/detection antibodies, respectively. The mean absorbance values of triplicate determinations are shown. Bars, SE.

Cheng, T. L., Cheng, C. M., Chen, B. M., Tsao, D. A., Chuang, K. H., Hsiao, S. W., ... & Roffler, S. R. (2005). Monoclonal antibody-based quantitation of poly (ethylene glycol)-derivatized proteins, liposomes, and nanoparticles. Bioconjugate chemistry, 16(5), 1225-1231.

Figure 1 Human Anti-PEG Recombinant Antibody (FAMAB-1525CQ) in SDS-PAGE

SDS-PAGE analysis of FAMAB-1525CQ in β-mercaptoethanol-reduced (Lane 1) and non-reduced (Lane 2) conditions. Gel stained for 30 minutes with Coomassie Blue. As a result of different β-mercaptoethanol-reduced proteins (Heavy chain and Light chain) migrate as about 50 kDa and 25 kDa, respectively.

Figure 2 Human Anti-PEG Recombinant Antibody (FAMAB-1525CQ) in SEC-HPLC

The purity of FAMAB-1525CQ was greater than 95% as determined by SEC-HPLC.

Column: 3 µm, 7.8 x 300 nm
Mobile phase: 150 mM Sodium Phosphate Buffer, pH 7.0
Detection: UV 280 nm
Injection: 10 µl

Figure 3 Human Anti-PEG Recombinant Antibody (FAMAB-1525CQ) in DB

Dot Blot analysis of FAMAB-1525CQ was performed by coating with PEG6000.

FAMAB-1525CQ incubation concentration: 2 ng/μL.
The secondary antibody: HRP-Goat Anti-Human IgG Fc antibody

Figure 4 Human Anti-PEG Recombinant Antibody (FAMAB-1525CQ) in ELISA

ELISA analysis of FAMAB-1525CQ was performed by coating with PEG6000. Then blocked with BSA and incubated with FAMAB-1525CQ. The HRP-conjugated goat anti-human IgG Fc as a secondary antibody (1: 2000). Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.