Human Anti-PEG Recombinant Antibody (clone E11) (CAT#: FAMAB-1525CQ)
This product is a recombinant human antibody that recognizes PEG. The antibody was purified by affinity chromatography. It can be used in WB, ELISA, FC.
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SDS-PAGE
Figure 1 Human Anti-PEG Recombinant Antibody (FAMAB-1525CQ) in SDS-PAGE
SDS-PAGE analysis of FAMAB-1525CQ in β-mercaptoethanol-reduced (Lane 1) and non-reduced (Lane 2) conditions. Gel stained for 30 minutes with Coomassie Blue. As a result of different β-mercaptoethanol-reduced proteins (Heavy chain and Light chain) migrate as about 50 kDa and 25 kDa, respectively.
SEC-HPLC
Figure 2 Human Anti-PEG Recombinant Antibody (FAMAB-1525CQ) in SEC-HPLC
The purity of FAMAB-1525CQ was greater than 95% as determined by SEC-HPLC.
Column: 3 µm, 7.8 x 300 nm
Mobile phase: 150 mM Sodium Phosphate Buffer, pH 7.0
Detection: UV 280 nm
Injection: 10 µl
DB
Figure 3 Human Anti-PEG Recombinant Antibody (FAMAB-1525CQ) in DB
Dot Blot analysis of FAMAB-1525CQ was performed by coating with PEG6000.
FAMAB-1525CQ incubation concentration: 2 ng/μL.
The secondary antibody: HRP-Goat Anti-Human IgG Fc antibody
ELISA
Figure 4 Human Anti-PEG Recombinant Antibody (FAMAB-1525CQ) in ELISA
ELISA analysis of FAMAB-1525CQ was performed by coating with PEG6000. Then blocked with BSA and incubated with FAMAB-1525CQ. The HRP-conjugated goat anti-human IgG Fc as a secondary antibody (1: 2000). Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.
Specifications
- Immunogen
- PEG-modified E. coli β-glucuronidase
- Host Species
- Human
- Type
- Human IgG1
- Specificity
- The repeating subunits of the PEG backbone
- Clone
- E11
- Applications
- WB, ELISA, FC
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
- Alternative Names
- Polyethylene Glycol
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2659 | Hi-Affi™ Recombinant Rabbit Anti-PEG Monoclonal Antibody (DS2659AB) | ELISA, WB, IHC-P | IgG |
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To accurately reference this product in your publication, please use the following citation information:
(Creative Biolabs Cat# FAMAB-1525CQ, RRID: AB_3111335)
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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