This product is a recombinant human antibody that recognizes PEG. The antibody was purified by affinity chromatography. It can be used in WB, ELISA, FC.
Figure 1 Immunoblot of free PEG and a PEG-modified enzyme.
Immunoblot of free PEG and a PEG-modified enzyme. Samples were electrophoresed on a 8% reduced SDS polyacrylamide gel, transferred to nitrocellulose paper, and probed with mAb E11. Lane 1, βG; lane 2, 3.5DNS-PEG3400-βG; lane 3, NH2-PEG1500-NH2; lane 4, CH3-PEG2000-OH. kDa, molecular mass in thousands.
Cheng, T. L., Cheng, C. M., Chen, B. M., Tsao, D. A., Chuang, K. H., Hsiao, S. W., ... & Roffler, S. R. (2005). Monoclonal antibody-based quantitation of poly (ethylene glycol)-derivatized proteins, liposomes, and nanoparticles. Bioconjugate chemistry, 16(5), 1225-1231.
Figure 2 Qualitative analysis of PEG-derivatized molecules.
Qualitative analysis of PEG-derivatized molecules. The binding of E11 to PEG and PEG-modified βG coated in microplates was detected by ELISA. The mean absorbance values (405 nm) of triplicate determinations are shown. Bars, SD.
Cheng, T. L., Cheng, C. M., Chen, B. M., Tsao, D. A., Chuang, K. H., Hsiao, S. W., ... & Roffler, S. R. (2005). Monoclonal antibody-based quantitation of poly (ethylene glycol)-derivatized proteins, liposomes, and nanoparticles. Bioconjugate chemistry, 16(5), 1225-1231.
Figure 3 Quantitative analysis of PEG-derivatized molecules by ELISA.
Graded concentrations of 3.5DNS-PEG3400-âG were assayed with a sandwich ELISA in which E11/AGP3 (●) or AGP3/E11 (○) were used for the capture/detection antibodies, respectively. The mean absorbance values of triplicate determinations are shown. Bars, SE.
Cheng, T. L., Cheng, C. M., Chen, B. M., Tsao, D. A., Chuang, K. H., Hsiao, S. W., ... & Roffler, S. R. (2005). Monoclonal antibody-based quantitation of poly (ethylene glycol)-derivatized proteins, liposomes, and nanoparticles. Bioconjugate chemistry, 16(5), 1225-1231.
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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CAT | Product Name | Application | Type |
---|---|---|---|
MOR-2659 | Hi-Affi™ Recombinant Rabbit Anti-PEG Monoclonal Antibody (DS2659AB) | ELISA, WB, IHC-P | IgG |
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(Creative Biolabs Cat# FAMAB-1525CQ, RRID: AB_3111335)
For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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