Rabbit Anti-PF4 Recombinant Antibody (clone R02-1E3) (CAT#: VS3-FY2063)
Clone R02-1E3 is a rabbit recombinant monoclonal antibody that specifically directed against human PF4. It cross-reacts with rat. It is suitable for use in a variety of assays, including WB, IHC-P, IP. This PF4 antibody was used in western blot analysis of PF4 in rat spleen lysates and western blot analysis of PF4 in A549, HL-60 lysates.
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WB
Figure 1 Anti-PF4 Antibody (VS3-FY2063) in WB.
The PF4 expression in rat spleen lysates were detected using the anti-PF4 by western blot assay.
WB
Figure 2 Anti-PF4 Antibody (VS3-FY2063) in WB.
The PF4 expression in A549, HL-60 lysates were detected using the anti-PF4 by western blot assay.
Specifications
- Immunogen
- Synthetic peptide of human PF4
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human and Rat PF4
- Species Reactivity
- Human, Rat
- Clone
- R02-1E3
- Applications
- Western Blot, Immunohistochemistry-Paraffin, Immunoprecipitation
- MW
- The calculated weight of the target protein is approximately 11 kDa, while the observed molecular weight stands at 11 kDa.
- Related Disease
- Erythromelalgia and Thrombocytopenia
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry: 1/50-1/100
Immunoprecipitation: 1/20
Target
- Alternative Names
- Platelet Factor 4; Chemokine (C-X-C Motif) Ligand 4; C-X-C Motif Chemokine 4; Oncostatin-A; Iroplact; CXCL4; SCYB4; PF-4
- Gene ID
- 5196
- UniProt ID
- P02776
- Sequence Similarities
- Belongs to the intercrine alpha (chemokine CxC) family.
- Cellular Localization
- Secreted
- Post Translation Modifications
- Modification sites at PhosphoSitePlus (P02776)
Modification sites at neXtProt (NX_P02776)
- Protein Refseq
- NP_001350281.1; NP_002610.1
- Function
- Released during platelet aggregation. Neutralizes the anticoagulant effect of heparin because it binds more strongly to heparin than to the chondroitin-4-sulfate chains of the carrier molecule. Chemotactic for neutrophils and monocytes. Inhibits endothelial cell proliferation, the short form is a more potent inhibitor than the longer form.
Recommended Products
Isotype Control
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Rabbit Monoclonal Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NAB-1874-VHH | Recombinant Anti-Human PF4 VHH Single Domain Antibody | IHC, IP, FC, Neut, FUNC | Llama VHH |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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