Rabbit Anti-PF4 Recombinant Antibody (clone R02-1E3)
CAT#: VS3-FY2063
Clone R02-1E3 is a rabbit recombinant monoclonal antibody that specifically directed against human PF4. It cross-reacts with rat. It is suitable for use in a variety of assays, including WB, IHC-P, IP. This PF4 antibody was used in western blot analysis of PF4 in rat spleen lysates and western blot analysis of PF4 in A549, HL-60 lysates.
Gene Expression
Tested Data
Normal Tissue
Figure 1 Bone marrow
Hematopoietic cells
Staining:Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/52485/126143_B_6_4.jpg
RNA Expression
Figure 2 RNA cell line category: Cell line enriched (HEL)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000163737-PF4
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WB
Figure 1 Anti-PF4 Antibody (VS3-FY2063) in WB.
The PF4 expression in rat spleen lysates were detected using the anti-PF4 by western blot assay.
WB
Figure 2 Anti-PF4 Antibody (VS3-FY2063) in WB.
The PF4 expression in A549, HL-60 lysates were detected using the anti-PF4 by western blot assay.
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Specifications
- Immunogen
- Synthetic peptide of human PF4
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human and Rat PF4
- Species Reactivity
- Human, Rat
- Clone
- R02-1E3
- Applications
- Western Blot, Immunohistochemistry-Paraffin, Immunoprecipitation
- MW
- The calculated weight of the target protein is approximately 11 kDa, while the observed molecular weight stands at 11 kDa.
- Related Disease
- Erythromelalgia and Thrombocytopenia
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry: 1/50-1/100
Immunoprecipitation: 1/20
Target
- Alternative Names
- Platelet Factor 4; Chemokine (C-X-C Motif) Ligand 4; C-X-C Motif Chemokine 4; Oncostatin-A; Iroplact; CXCL4; SCYB4; PF-4
- Gene ID
- 5196
- UniProt ID
- P02776
- Sequence Similarities
- Belongs to the intercrine alpha (chemokine CxC) family.
- Cellular Localization
- Secreted
- Post Translation Modifications
- Modification sites at PhosphoSitePlus (P02776)
Modification sites at neXtProt (NX_P02776)
- Protein Refseq
- NP_001350281.1; NP_002610.1
- Function
- Released during platelet aggregation. Neutralizes the anticoagulant effect of heparin because it binds more strongly to heparin than to the chondroitin-4-sulfate chains of the carrier molecule. Chemotactic for neutrophils and monocytes. Inhibits endothelial cell proliferation, the short form is a more potent inhibitor than the longer form.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| PFBL-299 | Mouse Anti-PF4 Recombinant Antibody (clone KKO); Fab Fragment | ELISA, FuncS | Mouse Fab |
| HPAB-0065-WJ-F(E) | Mouse Anti-PF4 Recombinant Antibody (clone 16D6-3); Fab Fragment | ELISA, WB | Mouse Fab |
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| CAT | Product Name | Application | Type |
|---|---|---|---|
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| CAT | Product Name | Application | Type |
|---|---|---|---|
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| CAT | Product Name | Application | Type |
|---|---|---|---|
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